LOCUS       sequence-23407-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Takemaru K, Yamaguchi S, Lee YS, Zhang Y, Carthew RW, Moon RT
  TITLE     Chibby, a nuclear beta-catenin-associated antagonist of the 
            Wnt/Wingless pathway.
  JOURNAL   Nature. 2003 Apr 24. 422(6934):905-9.
  PUBMED    12712206
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2003 Apr 24. 422(6934):905-9."
COMMENT     Sequence Label: XE226 Drosophila Chibby-pCS2P+
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(362..381)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(363..381)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    386..520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(410..429)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     464..483
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(633..653)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(633..651)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(672..688)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(672..688)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(685..707)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    696..712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(720..726,727..744,745..749))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tcttttgcgg atcctcagtt gtttgcaatg ccgctcttca acaagaagtt cgagtcgaag
       61 ccgattccag tgcgtcaggg acgttgcaat ataggacatc cggtggccac cgaggatctg
      121 gatgacttcc gccagatttc cctaacgctg ggcaacaagg agctgcgttt tgcggacggc
      181 atctggatgc attcgacccg aaagggtgac gtagatgata tgctccggct gaacaagaag
      241 tttcgtgccc tcgaggagga gaacaatatg tgtaatctta agatcgaggt gatgctggat
      301 ctgctggccg agcgcgacgg aactaagaat tcaaggcctc tcgagcctct agattctgca
      361 gccctatagt gagtcgtatt acgtagatcc agacatgata agatacattg atgagtttgg
      421 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      481 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
      541 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taattcgcgg ccgcggcgcc
      601 aatgcattgg gcccggtacc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
      661 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      721 aacatacgag ccgggagcat aaagtgtaag cctggggtgc ctaatgagtg agctaactcn
      781 cattattgcg ttgcgctcnc tgcccgct
//
