LOCUS       sequence-234071-        7260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_021730-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2418..2435
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2487..2542
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2541..2560)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4778..4796)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4864..4968
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4969..5037,5038..5829)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5187..5206)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6000..6588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6489..6508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6742..6759
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6876..6897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6912..6917,6918..6935,6936..6942)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6950..6966
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6955..6977
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6974..6990
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6974..6990
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccggcggaa
     1201 tcaaggaccg gatgctgaag ttcatcaccg acaaccggaa cctgttcgac accgtggtgt
     1261 ccggcgacct gaacgtgccc gtggaaagca agctgagctg gctgcaggaa gatctgatcg
     1321 ccgagatcag cgaggtggaa cccgacacca gcagaggcga gatcaagagc agcaccaaag
     1381 aaaaccccaa cctggtggcc agcagcaacg tgcgggccgt gcagcagtgc cactgcagcc
     1441 ctcacgacca ccacctgaag tccctggtgc tgtacagaaa cctgcccgcc agatgcttct
     1501 gcggccactg gtttctgctg gtggacgagc agagatacga agaggaacgc gagaagaagt
     1561 ggaacaagat caaggacaag cccgagaacg ccgacctgct gaagagattc gacatcctgg
     1621 aaaaagagct gaacatcctg ctggccgagg gcaagagcat gacccccaag agccctggcg
     1681 ccgacgagat gatggacaac atggccctga agtggaaaga gatgaaggaa gtctacgcca
     1741 acatccggaa gaacatgctg ctggatggcg cgccgtcgac ctccatcacg gcctataaga
     1801 gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa agcctgcagg
     1861 gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc accttctccc
     1921 taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc cagctgtccg
     1981 aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct ggttctggca
     2041 acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg gtggtgatga
     2101 aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc acctcaccca
     2161 agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg caggagaaag
     2221 tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt gaaggtgaag
     2281 aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc ggcagcctgc
     2341 accacatcct ggacgcccag aagatgctgt ggaaccacag ggacaggaac ctgcctcctc
     2401 tggcccctct gggcccccac caccatcacc accattgagt ctagacacgt gattaattaa
     2461 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     2521 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     2581 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     2641 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     2701 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     2761 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     2821 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     2881 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     2941 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     3001 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     3061 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     3121 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     3181 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     3241 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     3301 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     3361 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     3421 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     3481 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     3541 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     3601 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     3661 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     3721 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     3781 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     3841 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     3901 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     3961 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     4021 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     4081 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     4141 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     4201 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4261 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     4321 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4381 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     4441 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     4501 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     4561 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     4621 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     4681 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
     4741 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     4801 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4861 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4921 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4981 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5041 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5101 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     5161 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     5221 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5281 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5341 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5401 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5461 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     5521 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5581 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5641 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5701 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5761 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5821 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5881 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5941 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6121 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6781 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     6841 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     6901 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     6961 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
     7021 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     7081 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     7141 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     7201 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
//
