LOCUS       sequence-234072-        7611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_008320-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2769..2786
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2838..2893
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2892..2911)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5129..5147)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5215..5319
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5320..5388,5389..6180)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5538..5557)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6351..6939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6840..6859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7093..7110
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7227..7248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7263..7268,7269..7286,7287..7293)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7301..7317
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7306..7328
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7325..7341
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7325..7341
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccgataagg
     1201 cctgctgcct gatcagagcc gtgtacgaca gcaaggacct ggccatcatc gacaagggcg
     1261 tggacgacct gcagagcaga gccgaccggg acagccagcg gtgcatcgtg cagagcctga
     1321 acgagatctt ccccggcctg cacgtgatcg gcgaggaagg cgacctggac cccggcaacc
     1381 tgcccagaag caccgagctg aacctggccg tgctggaata ccagtgcccc cctgagctga
     1441 aggactgcag cctggacgac atcgtcgtgt gggtggaccc cctggacggc accaaagagt
     1501 tcaccgaggg cctgatcgag ttcgtgaccg tgctgatcgg catcagcctg gccggcaagc
     1561 ctgtgggcgg agtgatcgcc cagcccttct acaaggccaa ccccaccgcc aacaccaccg
     1621 accccaacta caccacccgg atcgtgtggg gcctcgtggg cctgggcgtg ttcggagtga
     1681 accccctgct gcccagcagc aagctgccct accccatcaa ccagaacgcc cagaagctga
     1741 ccagccccca catcatcgtg gtcacccggt cccacagaag cctgacccac gacttcgtgg
     1801 acggcgcctt ctaccccacc gaggtgctga gagccggcgg atgcggctac aaggtgctgg
     1861 tgctgctgga aggcagagcc cacgtgtacg tgttcccaag tcagggcacc aagaaatggg
     1921 acacctgtgc ccccgaggcc gtgctgaggg cctctggcgg aatcctgacc gacctgaccg
     1981 gccagcccta taagtacgac ctgaacgcca agcacgacaa cctgcggggc atcctggcca
     2041 cccctgtggc cgattggctg cccagctacg tgtcctgcct gcccaaagag gtgctggacg
     2101 acttcagcgc cagagatggc gcgccgtcga cctccatcac ggcctataag agtgaggggg
     2161 agtcagcgga gttctccttc ccactcaacc ttggagagga aagcctgcag ggagagttga
     2221 gatggaaggc agagaaggct ccttcttccc agtcctggat caccttctcc ctaaagaacc
     2281 aaaaggtgtc tgtgcagaag tctactagca accccaagtt ccagctgtcc gaaacgctcc
     2341 cactcaccct tcagataccc caggtctccc ttcagtttgc tggttctggc aacctgaccc
     2401 tgactctgga cagagggata ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc
     2461 agcccgacag caacactttg acctgtgagg tgatgggacc cacctcaccc aagatgagac
     2521 tgatcttgaa gcaggagaat caggaggcca gggtctccag gcaggagaaa gtgattcaag
     2581 tgcaggcccc tgaagcaggg gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga
     2641 tggactccaa gatccaggtt ttatccaaag ggttgaattc cggcagcctg caccacatcc
     2701 tggacgccca gaagatgctg tggaaccaca gggacaggaa cctgcctcct ctggcccctc
     2761 tgggccccca ccaccatcac caccattgag tctagacacg tgattaatta aggatccccc
     2821 gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     2881 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg
     2941 gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     3001 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     3061 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     3121 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     3181 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     3241 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     3301 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     3361 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     3421 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     3481 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     3541 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     3601 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     3661 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     3721 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     3781 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     3841 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     3901 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     3961 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     4021 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     4081 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     4141 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     4201 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     4261 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     4321 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     4381 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     4441 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     4501 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     4561 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4621 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     4681 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     4741 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     4801 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     4861 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     4921 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     4981 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     5041 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa
     5101 acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5161 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5221 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5281 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5341 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5401 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5461 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5521 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     5581 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5641 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5701 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5761 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5821 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     5881 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5941 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6001 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6061 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6121 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6181 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6241 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6301 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6361 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6421 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6481 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6541 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6601 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6661 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6721 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6781 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6841 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6901 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6961 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7021 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7081 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7141 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7201 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7261 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7321 cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc
     7381 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
     7441 agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag
     7501 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
     7561 atcataatat gtacatttat attggctcat gtccaatatg accgccatgt t
//
