LOCUS       sequence-234073-        3827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part. Promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie 
            MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick
            AJ
  TITLE     CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria 
            Based on the Plant MoClo Syntax.
  JOURNAL   Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub
            2019 Feb 28.
  PUBMED    30819783
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.18.01401"; journalName: "Plant Physiol"; date: 
            "2019-05"; volume: "180"; issue: "1"; pages: "39-55"
COMMENT     Sequence Label: pC0.055
FEATURES             Location/Qualifiers
     source          1..3827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(78..172)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(328..357)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     regulatory      798..807
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      890..921
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(1020..1835)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1743..1762
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1827..1846
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(2193..3143)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3191..3413
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 ttcagactgg ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta
       61 ctgtccctag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
      121 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
      181 ctcgtaaact tggtctgaca gctctagctc cggcaaaaaa acgggcaagg tgtcaccacc
      241 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga cgtctaagaa
      301 aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag gtgagccggt
      361 ctcaggagga gttgttcact tttttactga cgagggcttc cggaggccac gctcccaccc
      421 ataacagctt gccacatccc cgtcggaagt tacgttaccc ttgggcgatc gccaaaaatc
      481 agcatatata caccaattct aaataagatc ttttacaccg ctactgcaat caacctcatc
      541 aacaaaattc ccctctagca tccctggagg caaatcctca cctggccatg ggttcaaccc
      601 tgcttaacat ttcttaataa ttttagttgc tataaattct catttatgcc cctataataa
      661 ttcgggagta agtgctaaag attctcaact gctccatcag tggtttgagc ttagtcctag
      721 ggaaagattg gcgatcgccg ttgtggttaa gccagaataa agtctcgggt ggacagagaa
      781 cgctttattc tttgcctcca tggcggcatc ccacctaggt ttctcggcac ttattgccat
      841 aatttattat ttgtcgtctc aattaaggag gcaattctaa tgtgagacca gtcaaaagcc
      901 tccgaccgga ggcttttgac taaaacttcc cttggggtta tcattggggc tcactcaaag
      961 gcggtaatca gataaaaaaa atccttagct ttcgctaagg atgatttctg ctagtattat
     1021 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     1081 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccaa
     1141 agaatggcaa ggtcctggta acggtctgcg attccgaccc gtccaacatc aatacaacct
     1201 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     1261 gaatccggtg agaatggcaa gagcttgtgc atttctttcc agacttgttc aacaggccag
     1321 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat gcgtgattgc
     1381 gcctgagcaa gacgaaatac acgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     1441 tgtaaccggc gcaggaacac ggccagcgca tcaacaatat tttcacctga atcaggatat
     1501 tcttctaata cctggaaggc tgttttccca ggaatcgcgg tggtgagtaa ccacgcatca
     1561 tcaggagtac ggataaaatg cttgatggtc gggagaggca taaactccgt cagccagttg
     1621 agacggacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     1681 aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca
     1741 ttatcgcgag cccatttata cccatataaa tcagcgtcca tgttggagtt taagcgcgga
     1801 cgggagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     1861 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
     1921 agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat
     1981 cagctctagt agttacattg tcgatctgtt catggtgaac agctttgaat gcaccaaaaa
     2041 ctcgtaaaag ctctgatgta tctatctttt ttacaccgtt ttcatctgtg catatggaca
     2101 gttttccctt tgatatgtaa cggtgaacag ttgttctact tttgtttgtt agtcttgatg
     2161 cttcactgat agatacaaga gccataagaa cctcagatcc ttccgtattt agccagtatg
     2221 ttctctagtg tggttcgttg tttttgcgtg agccatgaga acgaaccatt gagatcatac
     2281 ttactttgca tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt
     2341 aaagcatcgt gtagtgtttt tcttagtccg ttatgtaggt aggaatctga tgtaatggtt
     2401 gttggtattt tgtcaccatt catttttatc tggttgttct caagttcggt tacgagatcc
     2461 atttgtctat ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca
     2521 accaccaatt tcatattgct gtaagtgttt aaatctttac ttattggttt caaaacccat
     2581 tggttaagcc ttttaaactc atggtagtta ttttcaagca ttaacatgaa cttaaattca
     2641 tcaaggctaa tctctatatt tgccttgtga gttttctttt gtgttagttc ttttaataac
     2701 cactcataaa tcctcataga gtatttgttt tcaaaagact taacatgttc cagattatat
     2761 tttatgaatt tttttaactg gaaaagataa ggcaatatct cttcactaaa aactaattct
     2821 aatttttcgc ttgagaactt ggcatagttt gtccactgga aaatctcaaa gcctttaacc
     2881 aaaggattcc tgatttccac agttctcgtc atcagctctc tggttgcttt agctaataca
     2941 ccataagcat tttccctact gatgttcatc atctgagcgt attggttata agtgaacgat
     3001 accgtccgtt ctttccttgt agggttttca atcgtggggt tgagtagtgc cacacagcat
     3061 aaaattagct tggtttcatg ctccgttaag tcatagcgac taatcgctag ttcatttgct
     3121 ttgaaaacaa ctaattcaga catacatctc aattggtcta ggtgatttta atcactatac
     3181 caattgagat gggctagtca atgataatta catgtccttt tcctttgagt tgtgggtatc
     3241 tgtaaattct gctagacctt tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat
     3301 tccgctagac ctttgtgtgt tttttttgtt tatattcaag tggttataat ttatagaata
     3361 aagaaagaat aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa ctcactactt
     3421 tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac
     3481 agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa atcgctgaat
     3541 attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg acattcagtt
     3601 cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc gccttttatg
     3661 gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct tctcagggcg
     3721 ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc agttcctgcc
     3781 ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtca
//
