LOCUS       sequence-234075-        7677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_166300-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2835..2852
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2904..2959
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2958..2977)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5195..5213)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5281..5385
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5386..5454,5455..6246)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5604..5623)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6417..7005
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6906..6925
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7159..7176
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7293..7314
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7329..7334,7335..7352,7353..7359)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7367..7383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7372..7394
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7391..7407
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7391..7407
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccaaccacg
     1201 agaagtgctt ttgctacctg aacagcacca ccaacctgcc ctaccacacc accaacaaca
     1261 ccaacgtgaa gcagctgatc cactgcagcc tgacccagct gagctacaac cacagcgagg
     1321 aactgagctg ccagcgggtg gacaagttca tctgcatcgg cccctacctg agcaacgaca
     1381 gccacatcaa cctgagcatc aacccctggg ccttcagcaa cctgttcgtg tccaacagcg
     1441 tgaacaccct gcagctggac ttcaacatca acacccacca gctgaacgcc ctgaccctgg
     1501 ccggcatgga cggcctgctg aacctgctgg ccagaaccag cctgctgagc tgggacagct
     1561 gcagcttcat cggcctgccc aacctgcacg agatcaccct gaactgcaag gccgtgttcc
     1621 ccgagttcct gaccttcggc tccttcatca ccctgatcag attcgtggac tgcgagggcg
     1681 tgcccctgca gttctactgc atccagtgta tccagaaccc caacgtgtac gtgatcagaa
     1741 tcagacccgg cccacccctg cggcagtacc tggtcaagtt cacccggctg agcagcagca
     1801 gcggcagctc caccagcagc ctgaacaaca agatcatcga gaacaacgag aacctgagcc
     1861 ccaaccacca tctgcccctg gacagctgcg tgtacggcgt gtgcagcgac gagatgctgt
     1921 gccgggacaa cctgcccctg aaacaggtgc aggccggcat cggccagccc gagaagccca
     1981 tcatcgagat cgtgtccaat gacgagatcc tgatccctaa caccaccagc atggacctgt
     2041 acgacagcat gcagctgacc agcatccagc ccatcctgaa caccaccccc agcatcatcg
     2101 agcccatcaa caccacctcc gtgtacgaca tcgtgatcct gaccaaatac ggccacagca
     2161 agaccgacca ccggcagaag attggcgcgc cgtcgacctc catcacggcc tataagagtg
     2221 agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc ctgcagggag
     2281 agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc ttctccctaa
     2341 agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag ctgtccgaaa
     2401 cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt tctggcaacc
     2461 tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg gtgatgaaag
     2521 tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc tcacccaaga
     2581 tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag gagaaagtga
     2641 ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa ggtgaagagg
     2701 tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccggc agcctgcacc
     2761 acatcctgga cgcccagaag atgctgtgga accacaggga caggaacctg cctcctctgg
     2821 cccctctggg cccccaccac catcaccacc attgagtcta gacacgtgat taattaagga
     2881 tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     2941 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat
     3001 ccctcggaga tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     3061 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     3121 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     3181 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     3241 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     3301 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     3361 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     3421 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     3481 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     3541 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     3601 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     3661 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     3721 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     3781 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     3841 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     3901 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     3961 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     4021 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     4081 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     4141 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     4201 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     4261 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     4321 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     4381 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     4441 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     4501 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     4561 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     4621 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4681 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     4741 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     4801 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
     4861 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     4921 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     4981 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     5041 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5101 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgataagc
     5161 tgtcaaacat gagaattttc ttgaagacga aagggcctcg tgatacgcct atttttatag
     5221 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5281 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5341 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5401 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5461 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5521 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5581 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     5641 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5701 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5761 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5821 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5881 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     5941 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6001 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6061 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6121 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6181 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6241 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6301 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6361 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6421 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6481 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6541 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6601 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6661 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6721 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6781 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6841 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6901 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6961 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7021 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7081 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7141 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7201 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7261 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7321 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7381 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     7441 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     7501 aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca
     7561 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
     7621 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgtt
//
