LOCUS       sequence-234081-        4678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Sousa MC, Trame CB, Tsuruta H, Wilbanks SM, Reddy VS, McKay DB
  TITLE     Crystal and solution structures of an HslUV protease-chaperone 
            complex.
  JOURNAL   Cell. 2000 Nov 10. 103(4):633-43.
  PUBMED    11106733
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2000 
            Nov 10. 103(4):633-43."
COMMENT     Sequence Label: HI.HslUV.RSET.FL.wt
FEATURES             Location/Qualifiers
     source          1..4678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      125..713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     614..633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     867..884
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     969..988
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        969..987
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1019..1041
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(3028..3046)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3042..3089
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3186..3641
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3273..3292)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3483..3504
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3667..3771
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3772..3840,3841..4632)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3990..4009)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
       61 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      121 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      181 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      241 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      301 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      361 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      421 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      481 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      541 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      601 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      661 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      721 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      781 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      841 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      901 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagg atctcgatcc
      961 cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta gaaataattt
     1021 tgtttaactt taagaaggag atatacatat gacaacgatt gtaagcgtac gtcgcaatgg
     1081 gcaagtcgtc gtaggcggtg atggacaagt ttctttaggc aataccgtaa tgaaaggtaa
     1141 tgctcgtaaa gtacgccgtt tatataatgg caaagtgctt gctggttttg cgggtggaac
     1201 ggcagatgca tttacacttt ttgaattatt tgaacgtaaa cttgaaatgc atcaagggca
     1261 tttgctaaaa agtgcggtgg aattagcgaa agattggcgt acagatcgcg cacttcgtaa
     1321 attagaagcg atgctaattg ttgcggatga aaaagaaagt ttaatcatca cgggtattgg
     1381 cgatgtggtt cagcccgaag aagatcaaat tttagcaatc ggatcgggtg gtaactatgc
     1441 gttatctgcg gcacgtgcgt tagtggaaaa tactgaactt tctgcacatg aaattgttga
     1501 aaaatcttta agaattgctg gtgatatttg cgtgttcaca aataccaatt tcactattga
     1561 agaattaccg aattaaggaa aaagctatgt ctgaaatgac tcctcgcgaa atagtttctg
     1621 aattagatca acatattatt ggtcaagccg atgcaaaacg tgctgtggcg attgcgttgc
     1681 gtaaccgttg gcgtcgtatg cagcttcaag aacctttgcg tcatgaagta actccaaaaa
     1741 atattttaat gattggccca actggtgtgg gtaaaactga aattgcgcgc cgtttagcaa
     1801 aattagctaa cgcaccattt atcaaagtgg aagcaacgaa atttactgaa gtaggttacg
     1861 tggggaaaga ggtggattcc attattcgtg atttaacgga tagtgcgatg aaacttgttc
     1921 gccaacaaga aattgcgaag aacagagcaa gagctgaaga cgtggctgaa gagcgtattt
     1981 tggatgcttt acttcctcca gcaaaaaatc agtggggcga agttgaaaac catgatagtc
     2041 acagcagtac tcgccaagcg ttccgtaaaa aattacgtga aggtcaatta gatgataaag
     2101 aaatcgaaat tgatgtgtct gctggcgtat ctatgggggt tgaaattatg gctcctccag
     2161 gcatggaaga aatgacaaat caactgcaat cattgttcca aaatttgggt tcagacaaaa
     2221 cgaaaaaacg caaaatgaaa attaaagatg cgttgaaagc gttgattgat gatgaagcag
     2281 cgaaattaat taaccctgaa gaacttaaac aaaaagccat tgatgctgtg gaacaaaatg
     2341 gtatcgtctt tatcgatgaa atcgacaaaa tttgtaagaa aggtgaatac agtggtgctg
     2401 atgtatcgcg tgaaggtgtg caacgtgatt tacttccgtt agtagaaggc tcgacagtta
     2461 gcaccaagca cggaatggtt aaaactgatc atatcttatt tattgcatct ggtgcattcc
     2521 aagttgctcg cccttcagat ttaattcctg aactacaagg gcgtttgccg attcgtgttg
     2581 aacttaccgc attaagtgcg gcggattttg agcgtatttt aacagagcca catgcttctt
     2641 taaccgaaca atataaagcg ttgatggcaa cagaaggggt gaatattgca ttcacaacag
     2701 atgcagtgaa gaaaattgct gaggcggcat tccgagtgaa tgaaaaaacg gaaaatatcg
     2761 gtgcacgccg tttgcatacg gtaatggagc gtttgatgga taaaatttct ttcagcgcaa
     2821 gcgacatgaa tggtcaaacg gtcaatattg atgcagctta tgttgcggat gcacttggcg
     2881 aagtggttga aaatgaagat ttgagccgtt ttattcttta aagataaaaa ttttacatat
     2941 aaaagtgcgg tagggtaccg agctcgaatt cgaagcttga tccggctgct aacaaagccc
     3001 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     3061 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatctggcg
     3121 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3181 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3241 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3301 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3361 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     3421 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     3481 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     3541 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     3601 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     3661 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3721 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3781 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3841 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3901 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     3961 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4021 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4081 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4141 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4201 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4261 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4321 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4381 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4441 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4501 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4561 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4621 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaac
//
