LOCUS       sequence-234085-       12142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses disordered protein HP1-alpha fused with fluorescent 
            protein miRFP670 and optogenetic protein Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen
            NS, Haataja M, Brangwynne CP
  TITLE     Liquid Nuclear Condensates Mechanically Sense and Restructure the 
            Genome.
  JOURNAL   Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 
            10.1016/j.cell.2018.10.057.
  PUBMED    30500535
REFERENCE   2  (bases 1 to 12142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.10"; journalName: "Cell"; date: "2018-11-29- 
            29"; volume: "175"; issue: "6"; pages: "1481-1491.e13"
COMMENT     Sequence Label: pHR-HP1-alpha-miRFP670-Cry2WT
FEATURES             Location/Qualifiers
     source          1..12142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      1391..1400
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1397..2341
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             2354..3847
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    3999..4587
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4052..4072)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4470..4481)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4590..4606)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4591..4607)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4697..4930
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4955..4973)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4956..4973)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5263..5282)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5496..5515)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5615..5637
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5675..5693)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5761..5865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5866..5934,5935..6726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6084..6103)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6897..7485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7386..7405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7639..7656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7726..7746)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7731..8060
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7911..8046
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9194..9259
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9389..9409
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9834..9968
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9871..9890)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9925..9944
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10136..10769
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10816..10941
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11438..11671
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11856..11900
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgacagtgga tccggagcta ccatgggaaa
      781 gaaaaccaag cggacagctg acagttcttc ttcagaggat gaggaggagt atgtcgtgga
      841 gaaggtgcta gacaggcgcg tggttaaggg acaagtggaa tatctactga agtggaaagg
      901 cttttctgag gagcacaata cttgggaacc tgagaaaaac ttggattgcc ctgagctaat
      961 ttctgaattt atgaaaaagt ataagaagat gaaggagggt gaaaataata aacccaggga
     1021 gaagtcagaa agtaacaaga ggaaatccaa tttctcaaac agtgccgatg acatcaaatc
     1081 taaaaaaaag agagagcaga gcaatgatat cgctcggggc tttgagagag gactggaacc
     1141 agaaaagatc attggggcaa cagattcctg tggtgattta atgttcctaa tgaaatggaa
     1201 agacacagat gaagctgacc tggttcttgc aaaagaagct aatgtgaaat gtccacaaat
     1261 tgtgatagca ttttatgaag agagactgac atggcatgca tatcctgagg atgcggaaaa
     1321 caaagagaaa gaaacagcaa agagcggaat tctgcagtcg acggtaccgc gggcccggga
     1381 tccaccggtc gccaccatgg tagcaggtca tgcctctggc agccccgcat tcgggaccgc
     1441 ctctcattcg aattgcgaac atgaagagat ccacctcgcc ggctcgatcc agccgcatgg
     1501 cgcgcttctg gtcgtcagcg aacatgatca tcgcgtcatc caggccagcg ccaacgccgc
     1561 ggaatttctg aatctcggaa gcgtactcgg cgttccgctc gccgagatcg acggcgatct
     1621 gttgatcaag atcctgccgc atctcgatcc caccgccgaa ggcatgccgg tcgcggtgcg
     1681 ctgccggatc ggcaatccct ctacggagta ctgcggtctg atgcatcggc ctccggaagg
     1741 cgggctgatc atcgaactcg aacgtgccgg cccgtcgatc gatctgtcag gcacgctggc
     1801 gccggcgctg gagcggatcc gcacggcggg ttcactgcgc gcgctgtgcg atgacaccgt
     1861 gctgctgttt cagcagtgca ccggctacga ccgggtgatg gtgtatcgtt tcgatgagca
     1921 aggccacggc ctggtattct ccgagtgcca tgtgcctggg ctcgaatcct atttcggcaa
     1981 ccgctatccg tcgtcgactg tcccgcagat ggcgcggcag ctgtacgtgc ggcagcgcgt
     2041 ccgcgtgctg gtcgacgtca cctatcagcc ggtgccgctg gagccgcggc tgtcgccgct
     2101 gaccgggcgc gatctcgaca tgtcgggctg cttcctgcgc tcgatgtcgc cgtgccatct
     2161 gcagttcctg aaggacatgg gcgtgcgcgc caccctggcg gtgtcgctgg tggtcggcgg
     2221 caagctgtgg ggcctggttg tctgtcacca ttatctgccg cgcttcatcc gtttcgagct
     2281 gcgggcgatc tgcaaacggc tcgccgaaag gatcgcgacg cggatcaccg cgcttgagag
     2341 cgcggccacg cgtatgaaga tggacaaaaa gactatagtt tggtttagaa gagacctaag
     2401 gattgaggat aatcctgcat tagcagcagc tgctcacgaa ggatctgttt ttcctgtctt
     2461 catttggtgt cctgaagaag aaggacagtt ttatcctgga agagcttcaa gatggtggat
     2521 gaaacaatca cttgctcact tatctcaatc cttgaaggct cttggatctg acctcacttt
     2581 aatcaaaacc cacaacacga tttcagcgat cttggattgt atccgcgtta ccggtgctac
     2641 aaaagtcgtc tttaaccacc tctatgatcc tgtttcgtta gttcgggacc ataccgtaaa
     2701 ggagaagctg gtggaacgtg ggatctctgt gcaaagctac aatggagatc tattgtatga
     2761 accgtgggag atatactgcg aaaagggcaa accttttacg agtttcaatt cttactggaa
     2821 gaaatgctta gatatgtcga ttgaatccgt tatgcttcct cctccttggc ggttgatgcc
     2881 aataactgca gcggctgaag cgatttgggc gtgttcgatt gaagaactag ggctggagaa
     2941 tgaggccgag aaaccgagca atgcgttgtt aactagagct tggtctccag gatggagcaa
     3001 tgctgataag ttactaaatg agttcatcga gaagcagttg atagattatg caaagaacag
     3061 caagaaagtt gttgggaatt ctacttcact actttctccg tatctccatt tcggggaaat
     3121 aagcgtcaga cacgttttcc agtgtgcccg gatgaaacaa attatatggg caagagataa
     3181 gaacagtgaa ggagaagaaa gtgcagatct ttttcttagg ggaatcggtt taagagagta
     3241 ttctcggtat atatgtttca acttcccgtt tactcacgag caatcgttgt tgagtcatct
     3301 tcggtttttc ccttgggatg ctgatgttga taagttcaag gcctggagac aaggcaggac
     3361 cggttatccg ttggtggatg ccggaatgag agagctttgg gctaccggat ggatgcataa
     3421 cagaataaga gtgattgttt caagctttgc tgtgaagttt cttctccttc catggaaatg
     3481 gggaatgaag tatttctggg atacactttt ggatgctgat ttggaatgtg acatccttgg
     3541 ctggcagtat atctctggga gtatccccga tggccacgag cttgatcgct tggacaatcc
     3601 cgcgttacaa ggcgccaaat atgacccaga aggtgagtac ataaggcaat ggcttcccga
     3661 gcttgcgaga ttgccaactg aatggatcca tcatccatgg gacgctcctt taaccgtact
     3721 caaagcttct ggtgtggaac tcggaacaaa ctatgcgaaa cccattgtag acatcgacac
     3781 agctcgtgag ctactagcta aagctatttc aagaacccgt gaagcacaga tcatgatcgg
     3841 agcagcagcc cgggatccac cggtcgccac cggttcaggg agcggatccg gctcagggag
     3901 tgcggccgca actcccacct gcaacatgcg tgactgactg aggccgcgac tctagagtcg
     3961 acctgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     4021 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4081 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4141 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4201 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4261 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4321 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4381 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     4441 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4501 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4561 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaggg aattaattcg
     4621 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     4681 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     4741 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     4801 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     4861 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4921 atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt
     4981 gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc
     5041 ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg
     5101 tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg
     5161 aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca
     5221 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     5281 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     5341 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     5401 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     5461 cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag
     5521 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     5581 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     5641 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     5701 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5761 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5821 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5881 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5941 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6001 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6061 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6121 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6181 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6241 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6301 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6361 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6421 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6481 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6541 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6601 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6661 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6721 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6781 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6841 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6901 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6961 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7021 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7081 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7141 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7201 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7261 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7321 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7381 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7441 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7501 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7561 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7621 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7681 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt
     7741 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7801 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7861 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     7921 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7981 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8041 gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga
     8101 ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa
     8161 atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac
     8221 actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc
     8281 acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa
     8341 caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc
     8401 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
     8461 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
     8521 atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc
     8581 actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg
     8641 atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
     8701 caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact
     8761 tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
     8821 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
     8881 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc
     8941 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc
     9001 gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat
     9061 ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat
     9121 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
     9181 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
     9241 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
     9301 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
     9361 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
     9421 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
     9481 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
     9541 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
     9601 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
     9661 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
     9721 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     9781 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     9841 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9901 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9961 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
    10021 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
    10081 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa
    10141 gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa
    10201 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
    10261 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
    10321 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
    10381 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
    10441 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
    10501 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
    10561 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
    10621 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    10681 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    10741 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
    10801 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
    10861 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
    10921 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
    10981 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
    11041 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
    11101 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    11161 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
    11221 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
    11281 gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
    11341 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
    11401 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
    11461 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
    11521 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
    11581 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
    11641 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
    11701 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
    11761 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
    11821 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
    11881 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
    11941 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
    12001 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
    12061 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
    12121 acagatccat tcgattagtg aa
//
