LOCUS       sequence-234088-       12184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses disordered protein FUS(1-214) fused with fluorescent 
            protein miRFP670 and optogenetic protein Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen
            NS, Haataja M, Brangwynne CP
  TITLE     Liquid Nuclear Condensates Mechanically Sense and Restructure the 
            Genome.
  JOURNAL   Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 
            10.1016/j.cell.2018.10.057.
  PUBMED    30500535
REFERENCE   2  (bases 1 to 12184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.10"; journalName: "Cell"; date: "2018-11-29- 
            29"; volume: "175"; issue: "6"; pages: "1481-1491.e13"
COMMENT     Sequence Label: pHR-FUSN-miRFP670-Cry2WT
FEATURES             Location/Qualifiers
     source          1..12184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1153..1164
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1439..2383
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             2396..3889
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    4041..4629
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4094..4114)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4512..4523)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4632..4648)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4633..4649)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4739..4972
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4997..5015)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4998..5015)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5305..5324)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5538..5557)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5657..5679
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5717..5735)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5803..5907
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5908..5976,5977..6768)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6126..6145)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6939..7527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7428..7447
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7681..7698
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7768..7788)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7773..8102
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7953..8088
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9236..9301
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9431..9451
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9876..10010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9913..9932)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9967..9986
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10178..10811
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10858..10983
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11480..11713
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11898..11942
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgacagtgga tccggagcta ccatggcctc
      781 aaacgattat acccaacaag caacccaaag ctatggggcc taccccaccc agcccgggca
      841 gggctattcc cagcagagca gtcagcccta cggacagcag agttacagtg gttatagcca
      901 gtccacggac acttcaggct atggccagag cagctattct tcttatggcc agagccagaa
      961 cacaggctat ggaactcagt caactcccca gggatatggc tcgactggcg gctatggcag
     1021 tagccagagc tcccaatcgt cttacgggca gcagtcctcc tatcctggct atggccagca
     1081 gccagctccc agcagcacct cgggaagtta cggtagcagt tctcagagca gcagctatgg
     1141 gcagccccag agtgggagct acagccagca gcctagctat ggtggacagc agcaaagcta
     1201 tggacagcag caaagctata atccccctca gggctatgga cagcagaacc agtacaacag
     1261 cagcagtggt ggtggaggtg gaggtggagg tggaggtaac tatggccaag atcaatcctc
     1321 catgagtagt ggtggtggca gtggtggcgg ttatggcaat caagaccaga gtggtggagg
     1381 tggcagcggt ggctatggac agcaggaccg tggaggaggc ggctccggag gcggaagcat
     1441 ggtagcaggt catgcctctg gcagccccgc attcgggacc gcctctcatt cgaattgcga
     1501 acatgaagag atccacctcg ccggctcgat ccagccgcat ggcgcgcttc tggtcgtcag
     1561 cgaacatgat catcgcgtca tccaggccag cgccaacgcc gcggaatttc tgaatctcgg
     1621 aagcgtactc ggcgttccgc tcgccgagat cgacggcgat ctgttgatca agatcctgcc
     1681 gcatctcgat cccaccgccg aaggcatgcc ggtcgcggtg cgctgccgga tcggcaatcc
     1741 ctctacggag tactgcggtc tgatgcatcg gcctccggaa ggcgggctga tcatcgaact
     1801 cgaacgtgcc ggcccgtcga tcgatctgtc aggcacgctg gcgccggcgc tggagcggat
     1861 ccgcacggcg ggttcactgc gcgcgctgtg cgatgacacc gtgctgctgt ttcagcagtg
     1921 caccggctac gaccgggtga tggtgtatcg tttcgatgag caaggccacg gcctggtatt
     1981 ctccgagtgc catgtgcctg ggctcgaatc ctatttcggc aaccgctatc cgtcgtcgac
     2041 tgtcccgcag atggcgcggc agctgtacgt gcggcagcgc gtccgcgtgc tggtcgacgt
     2101 cacctatcag ccggtgccgc tggagccgcg gctgtcgccg ctgaccgggc gcgatctcga
     2161 catgtcgggc tgcttcctgc gctcgatgtc gccgtgccat ctgcagttcc tgaaggacat
     2221 gggcgtgcgc gccaccctgg cggtgtcgct ggtggtcggc ggcaagctgt ggggcctggt
     2281 tgtctgtcac cattatctgc cgcgcttcat ccgtttcgag ctgcgggcga tctgcaaacg
     2341 gctcgccgaa aggatcgcga cgcggatcac cgcgcttgag agcgcggcca cgcgtatgaa
     2401 gatggacaaa aagactatag tttggtttag aagagaccta aggattgagg ataatcctgc
     2461 attagcagca gctgctcacg aaggatctgt ttttcctgtc ttcatttggt gtcctgaaga
     2521 agaaggacag ttttatcctg gaagagcttc aagatggtgg atgaaacaat cacttgctca
     2581 cttatctcaa tccttgaagg ctcttggatc tgacctcact ttaatcaaaa cccacaacac
     2641 gatttcagcg atcttggatt gtatccgcgt taccggtgct acaaaagtcg tctttaacca
     2701 cctctatgat cctgtttcgt tagttcggga ccataccgta aaggagaagc tggtggaacg
     2761 tgggatctct gtgcaaagct acaatggaga tctattgtat gaaccgtggg agatatactg
     2821 cgaaaagggc aaacctttta cgagtttcaa ttcttactgg aagaaatgct tagatatgtc
     2881 gattgaatcc gttatgcttc ctcctccttg gcggttgatg ccaataactg cagcggctga
     2941 agcgatttgg gcgtgttcga ttgaagaact agggctggag aatgaggccg agaaaccgag
     3001 caatgcgttg ttaactagag cttggtctcc aggatggagc aatgctgata agttactaaa
     3061 tgagttcatc gagaagcagt tgatagatta tgcaaagaac agcaagaaag ttgttgggaa
     3121 ttctacttca ctactttctc cgtatctcca tttcggggaa ataagcgtca gacacgtttt
     3181 ccagtgtgcc cggatgaaac aaattatatg ggcaagagat aagaacagtg aaggagaaga
     3241 aagtgcagat ctttttctta ggggaatcgg tttaagagag tattctcggt atatatgttt
     3301 caacttcccg tttactcacg agcaatcgtt gttgagtcat cttcggtttt tcccttggga
     3361 tgctgatgtt gataagttca aggcctggag acaaggcagg accggttatc cgttggtgga
     3421 tgccggaatg agagagcttt gggctaccgg atggatgcat aacagaataa gagtgattgt
     3481 ttcaagcttt gctgtgaagt ttcttctcct tccatggaaa tggggaatga agtatttctg
     3541 ggatacactt ttggatgctg atttggaatg tgacatcctt ggctggcagt atatctctgg
     3601 gagtatcccc gatggccacg agcttgatcg cttggacaat cccgcgttac aaggcgccaa
     3661 atatgaccca gaaggtgagt acataaggca atggcttccc gagcttgcga gattgccaac
     3721 tgaatggatc catcatccat gggacgctcc tttaaccgta ctcaaagctt ctggtgtgga
     3781 actcggaaca aactatgcga aacccattgt agacatcgac acagctcgtg agctactagc
     3841 taaagctatt tcaagaaccc gtgaagcaca gatcatgatc ggagcagcag cccgggatcc
     3901 accggtcgcc accggttcag ggagcggatc cggctcaggg agtgcggccg caactcccac
     3961 ctgcaacatg cgtgactgac tgaggccgcg actctagagt cgacctgcag gcatgcaagc
     4021 ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     4081 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4141 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4201 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4261 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4321 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4381 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4441 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4501 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4561 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4621 cctccccgca tcgataccgt cgacctcgag ggaattaatt cgagctcggt acctttaaga
     4681 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     4741 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     4801 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4861 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4921 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
     4981 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
     5041 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
     5101 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
     5161 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa
     5221 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     5281 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     5341 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     5401 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     5461 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     5521 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     5581 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     5641 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     5701 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5761 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5821 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5881 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5941 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6001 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6061 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6121 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6181 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6241 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6301 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6361 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6421 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6481 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6541 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6601 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6661 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6721 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6781 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6841 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6901 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6961 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7021 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7081 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7141 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7201 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7261 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7321 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7381 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7441 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7501 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7561 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7621 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7681 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7741 cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     7801 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     7861 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7921 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     7981 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     8041 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     8101 aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg
     8161 tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg
     8221 ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat
     8281 aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag
     8341 atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt
     8401 gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc
     8461 atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc
     8521 tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg
     8581 gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg
     8641 caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct
     8701 ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat
     8761 cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag
     8821 tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg
     8881 ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc
     8941 gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg
     9001 attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag
     9061 gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt
     9121 ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta
     9181 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
     9241 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
     9301 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
     9361 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
     9421 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
     9481 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
     9541 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
     9601 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
     9661 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
     9721 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca
     9781 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     9841 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     9901 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9961 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    10021 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    10081 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    10141 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
    10201 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
    10261 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
    10321 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
    10381 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
    10441 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
    10501 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
    10561 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
    10621 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    10681 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    10741 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    10801 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
    10861 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
    10921 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
    10981 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
    11041 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
    11101 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
    11161 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
    11221 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
    11281 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
    11341 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
    11401 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
    11461 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
    11521 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
    11581 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
    11641 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
    11701 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
    11761 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
    11821 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
    11881 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
    11941 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
    12001 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
    12061 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
    12121 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
    12181 tgaa
//
