LOCUS       sequence-234089-        8385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_090110-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3543..3560
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3612..3667
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3666..3685)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5903..5921)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5989..6093
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6094..6162,6163..6954)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6312..6331)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7125..7713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7614..7633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7867..7884
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8001..8022
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8037..8042,8043..8060,8061..8067)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8075..8091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8080..8102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8099..8115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8099..8115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccgagtata
     1201 tctgccagct gatgcccaac atcacaatgg tgtccatgat cgtgttcaag acccggatga
     1261 gcctgttcaa gaacttcggc atcctgggcg agcccatcga caagagacag tacggcggca
     1321 accggctgaa gttcatcccc atctacagcc ggaacatgga aatgcacccc ctgttcagca
     1381 gattcaagac cgaggtggtg gccgtggccc tggccttttg ggagaaagct ctgagcgtgc
     1441 ggaagccccc caaccggaag ctgctgattg agcggggctg cgtggaaccc agctacttca
     1501 gagacagaag caccggcaag ctgttctgca agagccagtg cagacccaga gccctgtgct
     1561 acgatcacga ggtgcccaac gagtacgcca gcggatgtgc tgtgggcaca ggcagcggct
     1621 acatccggga cgtgtaccag gatggctccg gcttcgcccc caatgagtac gtgctgttca
     1681 tcagcagcga caacaagaag ggctgcctga gcggcgtgac actggcctat gccggacctt
     1741 gcgagatgca ccccaccacc gacagaccca ttatgggcgc tatcaacttc tgtccacaaa
     1801 agatggaaat cgaggaaccc ggcaagacca tgctgatcgg caccgccatt cacgagctgg
     1861 gccacgccat gcagatcaag atcagctata tcttcatctt ccggtttctg aacttcttct
     1921 tcgtggtgtt ccacttcagc gagaacaccg tgaagcggat cgtgcggcct tgggtcacag
     1981 ccgtgggcag cttcatcaag cccttcagca gcatcgtgac ccctgccctg ctggacgagg
     2041 gccggaagca ctacaactgc cccaacctgg acggcatcga cctggaaaat gagggcggcg
     2101 agggcacagt gggcagccac tttgagaagc gggtcgtggg cgacgagatc atggctggcg
     2161 tgaccggcgt gaaaaccgtg gtgtccagac tgaccctggc attcttccag gacagcgggt
     2221 ggtgggacgt gaactacagc gtggccgagg actggaacta cggcaagaac ctgggctgca
     2281 ccttcgtgcg cgagagctgc tacgcctaca tgatgaagat gaagctggaa ggcaagagca
     2341 tggaacccta ctgcgaagag gccgacagcc tgatgtgcta ccaccacaag gccttcggca
     2401 cctgtgccat cggccagtac atgaagtacc tgcagcccca ggaacagtac ttcaagggcg
     2461 ctcccgacta tggcggaaca ggcgccctgg tggataagtg ccctctgatc cagcccacca
     2521 agcagttctt tgaggaaccc ctgatgacct actgtgacca cagcctgaac atccctgtgg
     2581 cccagagagg caacatcttc ggccaggcct ttggcaacaa cagcgtgtgc atcacccaca
     2641 agggggcctg gaaggccaga ctgaatggca aggccaccca ggacccccgg atcaaggcca
     2701 catgtcacca gtacagctgc agcggcggag tgcaagtgat catcgacggc aagagcttca
     2761 gatgcgacaa cggcgtggcc aagatccaca ccgagagaat cgagggcgag gccatctgcc
     2821 ccaatcccaa cgacgtgtgc agagtgcgca tcgtgtacgt ggaacggaag gccgtggact
     2881 gcgagtgcat tggcgcgccg tcgacctcca tcacggccta taagagtgag ggggagtcag
     2941 cggagttctc cttcccactc aaccttggag aggaaagcct gcagggagag ttgagatgga
     3001 aggcagagaa ggctccttct tcccagtcct ggatcacctt ctccctaaag aaccaaaagg
     3061 tgtctgtgca gaagtctact agcaacccca agttccagct gtccgaaacg ctcccactca
     3121 cccttcagat accccaggtc tcccttcagt ttgctggttc tggcaacctg accctgactc
     3181 tggacagagg gatactgtat caggaagtga acctggtggt gatgaaagtg actcagcccg
     3241 acagcaacac tttgacctgt gaggtgatgg gacccacctc acccaagatg agactgatct
     3301 tgaagcagga gaatcaggag gccagggtct ccaggcagga gaaagtgatt caagtgcagg
     3361 cccctgaagc aggggtgtgg caatgtctac tgagtgaagg tgaagaggtc aagatggact
     3421 ccaagatcca ggttttatcc aaagggttga attccggcag cctgcaccac atcctggacg
     3481 cccagaagat gctgtggaac cacagggaca ggaacctgcc tcctctggcc cctctgggcc
     3541 cccaccacca tcaccaccat tgagtctaga cacgtgatta attaaggatc ccccgacctc
     3601 gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     3661 tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc ctcggagatc
     3721 tctagctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc
     3781 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
     3841 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
     3901 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
     3961 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
     4021 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
     4081 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
     4141 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
     4201 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
     4261 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
     4321 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
     4381 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
     4441 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
     4501 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
     4561 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
     4621 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
     4681 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
     4741 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
     4801 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
     4861 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
     4921 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
     4981 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
     5041 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
     5101 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
     5161 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
     5221 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
     5281 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     5341 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     5401 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     5461 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     5521 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
     5581 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
     5641 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     5701 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     5761 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     5821 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgataagctg tcaaacatga
     5881 gaattttctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5941 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6001 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6061 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6121 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6181 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6241 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6301 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     6361 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6421 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6481 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6541 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6601 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     6661 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6721 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6781 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6841 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6901 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6961 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7021 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7081 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7141 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7201 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7261 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7321 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7381 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7441 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7501 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7561 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7621 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7681 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7741 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7801 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7861 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7921 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     7981 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8041 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     8101 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacca attctcatgt
     8161 ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact
     8221 ggtaggtatg gaagatctat acattgaatc aatattggca attagccata ttagtcattg
     8281 gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata
     8341 atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgtt
//
