LOCUS       sequence-234090-        7653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7653)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_187140-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2811..2828
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2880..2935
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2934..2953)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5171..5189)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5257..5361
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5362..5430,5431..6222)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5580..5599)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6393..6981
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6882..6901
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7135..7152
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7269..7290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7305..7310,7311..7328,7329..7335)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7343..7359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7348..7370
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7367..7383
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7367..7383
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccaagcgga
     1201 ccctgaagtt caacgacctg tgggagaagt ggaagtccgt gtacaccaag cagtacaccg
     1261 acgaggacga ggaagccatc cggcggagaa tctggaacaa gaacctgatc aagatccagc
     1321 tgcacaacct gagatacgac ctgggcttcg agacatacag catcggcctg agccagttca
     1381 ccgacatcga gtggaacgag ttctacagca tgtacaaagt gaaagagcgg ctgatcatcc
     1441 ccgagagcag ccccagcagc agcttcatcg aagaggacta cgacgtgaac aacatcggct
     1501 ggacccccga cagctacgac tggcggcact tcaacatcgt gaacgagccc cgggacgagg
     1561 gcagctgcat cggcagctac gccttcgccg tgacagccgt gaccgagagc cagtacgccc
     1621 tgcggaccag caaccggaag aaattcagcg tgcagcagtt tatcgactgc acccggatct
     1681 acggcaacat gggctgccac ggcggctaca ccttcaccct gttcatctac ctgcagaact
     1741 tcggcctgga aaccgaggaa aactacccct tcactggcgg cgaccaggac tgcgtggcca
     1801 acagctccga cgtggtggtg cagagcatcg gctacaagtt ccaccggcac ggctacgaga
     1861 caatcctgaa gtgggccgtg tacaacgagg gcccctacgt gatcagcatg aatatcgacg
     1921 agaacttcct gcactacaag agcggcatct accagagcga tacctgcacc cactacaacc
     1981 tgaaccagag catgctgctc gtgggctacg gctacgacaa cgacggcaac gactactgga
     2041 ccctgcagaa caactggggc acaaagtggg gcgagcaggg ctacgtgaag gtgctgcgga
     2101 accactggaa catgtgcgga atcgccagca tggccttccg gcccatcctg cggggctttg
     2161 gcgcgccgtc gacctccatc acggcctata agagtgaggg ggagtcagcg gagttctcct
     2221 tcccactcaa ccttggagag gaaagcctgc agggagagtt gagatggaag gcagagaagg
     2281 ctccttcttc ccagtcctgg atcaccttct ccctaaagaa ccaaaaggtg tctgtgcaga
     2341 agtctactag caaccccaag ttccagctgt ccgaaacgct cccactcacc cttcagatac
     2401 cccaggtctc ccttcagttt gctggttctg gcaacctgac cctgactctg gacagaggga
     2461 tactgtatca ggaagtgaac ctggtggtga tgaaagtgac tcagcccgac agcaacactt
     2521 tgacctgtga ggtgatggga cccacctcac ccaagatgag actgatcttg aagcaggaga
     2581 atcaggaggc cagggtctcc aggcaggaga aagtgattca agtgcaggcc cctgaagcag
     2641 gggtgtggca atgtctactg agtgaaggtg aagaggtcaa gatggactcc aagatccagg
     2701 ttttatccaa agggttgaat tccggcagcc tgcaccacat cctggacgcc cagaagatgc
     2761 tgtggaacca cagggacagg aacctgcctc ctctggcccc tctgggcccc caccaccatc
     2821 accaccattg agtctagaca cgtgattaat taaggatccc ccgacctcga cctctggcta
     2881 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     2941 aaggacatat gggagggcaa atcatttggt cgagatccct cggagatctc tagctagagg
     3001 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     3061 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     3121 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     3181 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     3241 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     3301 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     3361 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     3421 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     3481 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     3541 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     3601 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     3661 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     3721 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     3781 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     3841 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     3901 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     3961 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     4021 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     4081 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     4141 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     4201 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     4261 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     4321 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     4381 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     4441 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     4501 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     4561 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     4621 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     4681 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     4741 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     4801 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
     4861 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     4921 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     4981 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     5041 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     5101 tgtatccggg tagcatatgc tatcctcatg ataagctgtc aaacatgaga attttcttga
     5161 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     5221 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5281 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5341 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5401 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5461 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5521 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5581 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     5641 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5701 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5761 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5821 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5881 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     5941 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6001 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6061 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6121 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6181 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6241 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6301 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6361 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6421 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6481 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6541 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6601 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6661 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6721 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6781 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6841 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6901 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6961 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7021 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7081 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7141 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     7201 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     7261 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     7321 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     7381 gaccatgatt acgccaagct ctagctagag gtcgaccaat tctcatgttt gacagcttat
     7441 catcgcagat ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg taggtatgga
     7501 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
     7561 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
     7621 atattggctc atgtccaata tgaccgccat gtt
//
