LOCUS       sequence-234129-       11616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 11616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_017730-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..11616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6780..6797
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6849..6904
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6903..6922)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9140..9158)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9226..9330
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9331..9399,9400..10191)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9549..9568)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10362..10950
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10851..10870
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11104..11121
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11238..11259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11274..11279,11280..11297,11298..11304)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11312..11328
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11317..11339
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11336..11352
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11336..11352
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 acctgcacag caacaacgtg gtggacatca aggcccacga ctacaagctg ctgaccaaga
     1261 tcctggccgc cagacagctg caggacctgt tcgacaacga caagaacacc cacggcctgt
     1321 tccacgccga gctgaaccag aaagtgtacc tgatcgtgga cctgggcggc ctgtaccagg
     1381 tgtccagcgt ggaaatcacc agcgacgagc ccgagaacct gaagcagacc atcagcatcg
     1441 gctacggctt cgacaagaat accaccagcc tgttcggcag cctgtacaac tgcacctacc
     1501 agcagaccag caccctgtgc atccccgcct gcagccagta cgacaacagc cggaccggct
     1561 tcgtgggcaa cagcctgctg tggtccttcc agagcgacaa agagggctgg acccggatct
     1621 acgacctgat catccggggc acccccttcg acgagaaccg gtccatgaag aacaacctgg
     1681 acgacatcac cctgttcaag ccctacgtgg accacctggt gcccgccgag agcatggaaa
     1741 gctgcaacgg actgctgtgg cacctgaacg acgagagcag cgtgaccgag tctagcgccc
     1801 tggccgctac cgacgaccgg aacgtgacca tctacttcgg caagacctac agcgtgacca
     1861 gcgtgcactt cgtgaccagc aagcgggacg acatgcccca ggagtacacc ctgcacttca
     1921 acggcatgga atccctcacc aagatcatca acctgcagaa cgactgcacc ctgaccgcca
     1981 gcagcgacaa cgacggcgcc aacttcaaag agtacaactg ccccaccacc gacctggaaa
     2041 gctacacctt cgactacgtg accgtgaacg tgaagggcct gtataagctg cacgtgtacg
     2101 gcctgccctt ccactacccc aagatccaga tcatccccag caaagaggac gacaagacca
     2161 tcatggtgga caagaacgtg ctgcagatca gctgtatcgc ccagagctgc aacagcacca
     2221 gcaacgtgct gctggacgtg gacgacagct accggcggag ccggtccagc gacaagagct
     2281 gccccatgaa cggccacatc gtcagatgcg agtacctgaa cctgttccgg ctgagcacca
     2341 gccccaacga cgacaacaac cagaagatcc agctgaccaa ccagggcatc atcaacaccg
     2401 agttcagcga gatcatgacc gagctgcgga tcatccagag caacgccgag aagctgatcg
     2461 tgaccatgcc ccggacccac cagtactact cccagtacga gtgcagctgc caggccaccg
     2521 acaacaagaa gtcccagctg ttcagcctgt ccaccaacct gaagcccacc gacttcgacc
     2581 aggacttcat ttttcagacc aactacacca ccatcttcgc cgaccagacc atcgagagcc
     2641 acgtgggctt catcgagctg cccgagaacg agcacgagag ctacttcaag ctgaacatca
     2701 tcggctactc cctgctgaac gacgtgcaga tcggcgtggt gttcgtggaa ggcggccagg
     2761 ccggcagccg gaccaacgcc aacatcgaag agatcagcgt gcaggccttc cccggcatca
     2821 acgtgggcag cgacctgtgg tccatccccg tgtataagta cgagctgcag gatgtgtggc
     2881 ccgacagcgt ggacgactcc gtgatcaccg tgacctggtc catgcccaag gccctgagcg
     2941 ccaccaagag ccccagcgtg atccgggaca tcttccgggc cctgatcatc accagcagcg
     3001 gcgtgaacac catccgggcc tgggtctggc agcgggacag ccatctgctg gacatcagag
     3061 cctaccacca gctggacgag aacaacgtgg acagccagct gaagatcctg accctgcagc
     3121 ggagcggctg cagccccagc gagtctgagg atgaggtggt ggccagcgtg cagctgaagg
     3181 acggccagtg cagcaccgat aacaacgaca tcatcacctg tacccggacc atccacggac
     3241 agatcatcca gtttaagctg aacaacccca gcacctccga cgtgtacaag ctgtacatga
     3301 agtccgacgg cgtggaagat aacgtggaaa gcacctccag catcgacctg gtcaccagcg
     3361 gcagcctggg cgagacagtg aaagaggata tcaagggcgc tggcctgagc ctgaccgtgg
     3421 aaggcatcca ccacaaccac gagacacagg aaaccgagct ggacgtcgcc gtgcacattg
     3481 ccagcaaagt gatcagcgac aatatcgcct gccggcccac ctacctgctg ctggaattca
     3541 tcgagcccaa catcgagaca ctgaagtccc gggtgtccag caagcagaca atgttccgga
     3601 tcaagctgcc cagcaaccag aaagaaatca acctgaagat gcagctctcc atcggcagcg
     3661 tggaccccac ccagagcgag gccaccacca accagtctat cgccttccag aaccccttct
     3721 acatccccac cgacatcaag gtggacgccg agaaccagct gatccagtgg ttcggcctgc
     3781 ctaccatctt caacaacctg ctgcaccact acgagacaaa gctgagcggc ctgccaaagg
     3841 cctgcgagca ggccagcgag ttcaacctgc ccatcaccca gcaggaaatc gacaacggca
     3901 ccatctacag agtgaacctg aagaacatcc ccgaccccac catcaccaag aacggcctgg
     3961 ccatcgacta caatttcaaa gtgacccccg tgttcaaggg catcgacggc aagagcatca
     4021 ccatgggcac cagctccgac atccggttca gcaccggcag aaccggccag accgacctga
     4081 aggcccccac cagcggacgg tactacagcc tgcaggtcca ggtccgaccc agccagatcc
     4141 ccagctgcct gaacctggaa accctgaaca cccagttcat cctgagagtg atcggcgagg
     4201 tggacgagta ccccgactac atcaagcagg tcaactacgt gccaatcacc atgaagacca
     4261 ccgagactct gaacgataag aacaatcacg tgaagctgta caagatcgag aacctgctgc
     4321 ccggcagaag atatgagctg caggccgaag tgatctacac cgaggacttc cgggacaaga
     4381 tcagcgagcc cgtgcggctg tggatcgagg acgaggtgca cgtgcagacc gaggaagtgt
     4441 tcatcagccc tggcgagcgg gtggtcatca actgcacagg cagcgtgggc cctaacgaca
     4501 ccagccagaa aagcctggaa tggaagctgt tcgacggcgg cagactgccc gacggcagca
     4561 gaagcctgaa aacccaggaa gcccagagcg gccctctgtg gtacgccatg gaaagcctga
     4621 tcttcgaccc cgtgaacaag cagcacggcg gcgtgtacgc ctgcttcatc cggcccagca
     4681 tcctggaact gatgaacaag cctaccgagc tgcacaaagt gaccgtgaca gtgtccgatc
     4741 tggaagtcga catcaacagc aagatcgtgg aattcggcga gaagatcatc atcacatgcc
     4801 ggaccgccag ccctggccag ctggactgga tgctgcctag cggcgagaag gtggaaatca
     4861 tgaacgagat gaagtctgac gacgacaatg acgaccagcc ctacaccatc aaggacgaga
     4921 acgacgatgt gaagctgagc atcaagctca tcatccccaa agtcaacctg aacaaagtgg
     4981 gcaagtacac ctgtctgcac agcccctcca acaacaagca gacctttagc ctgaagatga
     5041 aggaagtgat caagctggtc aagagccccg agtcctccga caagcccggc aagaccctga
     5101 tcctggactg caccgccaac ctgggcaacc tgcaccagag cgtcgtgtgg tacaagcggc
     5161 ccaacagcaa cagcccctgg ctggaaatca cagaggccat ccagacaatc gagcacatca
     5221 ccatccagca gaagaacccc gaggacaccc tgtccagcgg cgtgtggctg agcgagctga
     5281 aagtgaagaa cagccctggc atcatcggcg agttcatgtg caccatccag aacatccaga
     5341 ccaccatgaa catcgagcgg atggaaaccg gcagcatcat gaccaacgac aacgacttca
     5401 gcaagatcac ccacgccaca atcaaggtgt ccctgaagtc cgtgctgaaa attctgaccc
     5461 ccatcaagct ggaaaacggc cagatctccg tgcactgcca gggctacccc gcccacagca
     5521 aggaccggct gcagtgggtg tacatccccc tgaacacaga caacaagtcc gacaaagtga
     5581 ttaccatcgt gcactccaac cccaaggacg aggaaaacga ccaggaaagc gacggcaaca
     5641 ccaagtccct ggacgaaaaa gagatcgacg agatcgtcag cctggccttt cagctgaccg
     5701 actccatccc cgccacctgg cctggctcta caggccctca gcagctggtg cagagcggcc
     5761 tgatcgagca ggaacaccag cccaagcaga tgtacaccgc cgagcggctg tccctgatct
     5821 ttgactctaa gtacgccgag aaagtggccg acggcatcct gagctgcaga tacgtgcggc
     5881 ccaagggcat cctgcccatg gattccgatg aggccgccga gtccctgagc aaagtgacca
     5941 tccccgagac aaacgacgac agcgacgaga tcctggaaaa gtccgagatc cctatgaaga
     6001 ccctgctgga cgccaagcag ggcgacgaca acgataacct gagcatcctg aagtccagcc
     6061 tgaacgagga acccgatgag gtgcagtccg acgacaagaa gaacaacacc gagaagaagt
     6121 ccatctctgg cgcgccgtcg acctccatca cggcctataa gagtgagggg gagtcagcgg
     6181 agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg agatggaagg
     6241 cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac caaaaggtgt
     6301 ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc ccactcaccc
     6361 ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc ctgactctgg
     6421 acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact cagcccgaca
     6481 gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga ctgatcttga
     6541 agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa gtgcaggccc
     6601 ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag atggactcca
     6661 agatccaggt tttatccaaa gggttgaatt ccggcagcct gcaccacatc ctggacgccc
     6721 agaagatgct gtggaaccac agggacagga acctgcctcc tctggcccct ctgggccccc
     6781 accaccatca ccaccattga gtctagacac gtgattaatt aaggatcccc cgacctcgac
     6841 ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     6901 ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc ggagatctct
     6961 agctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     7021 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     7081 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     7141 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     7201 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     7261 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     7321 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     7381 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     7441 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     7501 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     7561 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     7621 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     7681 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     7741 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     7801 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     7861 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     7921 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     7981 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     8041 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     8101 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     8161 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     8221 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     8281 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     8341 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     8401 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     8461 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     8521 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     8581 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     8641 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     8701 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     8761 tgctatccta atagagatta gggtagtata tgctatccta atttatatct gggtagcata
     8821 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcatatgct
     8881 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     8941 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     9001 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     9061 atcctaatct gtatccgggt agcatatgct atcctcatga taagctgtca aacatgagaa
     9121 ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     9181 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     9241 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     9301 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     9361 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     9421 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     9481 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     9541 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     9601 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     9661 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     9721 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     9781 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     9841 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     9901 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     9961 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
    10021 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
    10081 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
    10141 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
    10201 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
    10261 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    10321 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    10381 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    10441 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    10501 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    10561 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    10621 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    10681 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    10741 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    10801 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    10861 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    10921 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    10981 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    11041 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    11101 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
    11161 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
    11221 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
    11281 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    11341 aacagctatg accatgatta cgccaagctc tagctagagg tcgaccaatt ctcatgtttg
    11401 acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt
    11461 aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
    11521 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
    11581 tgtacattta tattggctca tgtccaatat gaccgc
//
