LOCUS       sequence-234133-        7824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_011680-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1813..1822
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2602..2619
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2671..2726
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2725..2744)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4962..4980)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5048..5152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5153..5221,5222..6013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5371..5390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6184..6772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6673..6692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6926..6943
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7060..7081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7096..7101,7102..7119,7120..7126)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7134..7150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7139..7161
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7158..7174
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7158..7174
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7445..7824
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccctgttca ccttcaacca cctgttcgcc
      841 agcctgatca gccggaagct ggccatcctg cccggcagcg agatcttcga caactggaag
      901 tcccccagcg tgcccgtgta cttcagcatc tacctgtaca acctgaccaa ccccgaggaa
      961 gtgctgagcg gcggcagacc cagattcgac gaagtgggcc cctacgtgta cagagaggac
     1021 cggcagcgga acaacgtgga attcagcgag gaaagccccc ccaagaccgt gaagtaccag
     1081 caccggatcc tgtactactt ccagcccgag ctgagcgtgg gccccgacga tcagggcatc
     1141 gtgaccagcc tggacctggt caccgtggcc atcaacaacc tgcccgagta cctgaagatc
     1201 gccttcttcc tgtacacctt taacgccttc gtgtccaaga cccccagaga gatcatctgg
     1261 ggctacgagg accccctgat gagcgcctgc ctgagctacg gcacctgtga caccgacaga
     1321 gccggcctga tggtcaccaa gaacggcacc aaggtgtccg acttcgtgat cgacaccggc
     1381 gcctacaaca tcagcaacgt gggcaaggtg ctgcggtaca acggcgagac aagcctgaac
     1441 tactggcaca ccgactacgc caacatgatc aacggcaccg acggcagcgt gatcagaccc
     1501 ggcctgcaga tgagcagccg gatcttcttc ttcgtgcccg acttctgccg gtccttccac
     1561 agcgacgccg tgggctgggc caccgccaca cacgatagcg gagtgcatct gctgagattc
     1621 gccagccacc ccgagcagag ccagaacgcc accgtgaacc ccctgaacgc cgccttctgc
     1681 cccaagaaga aggccggacc cgactgcccc cccaccggca tgattcctct gagcccctgc
     1741 agcaaccccc ggatcgccgt gcctatcttc gcctgccagc ctcacttcct gggcgccgac
     1801 cctagcatca gagccgccat ggacggcatc cgcgagcccg acgtgaagca cgacagcacc
     1861 atcctgctga tcgagccctt cgccgtggcc gacaagaagg ccctgagcaa gatcagcaac
     1921 tacatcctga agcccaagaa gatcatccct ggcgcgccgt cgacctccat cacggcctat
     1981 aagagtgagg gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg
     2041 cagggagagt tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc
     2101 tccctaaaga accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg
     2161 tccgaaacgc tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct
     2221 ggcaacctga ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg
     2281 atgaaagtga ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca
     2341 cccaagatga gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag
     2401 aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt
     2461 gaagaggtca agatggactc caagatccag gttttatcca aagggttgaa ttccggcagc
     2521 ctgcaccaca tcctggacgc ccagaagatg ctgtggaacc acagggacag gaacctgcct
     2581 cctctggccc ctctgggccc ccaccaccat caccaccatt gagtctagac acgtgattaa
     2641 ttaaggatcc cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag
     2701 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg
     2761 tcgagatccc tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc
     2821 tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt
     2881 ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa
     2941 ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga
     3001 gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta
     3061 tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc
     3121 ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat
     3181 aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa
     3241 acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat
     3301 agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta
     3361 aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga
     3421 ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg
     3481 gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa
     3541 taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt
     3601 gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca
     3661 atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa
     3721 tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct
     3781 cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat
     3841 taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta
     3901 acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc
     3961 ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc
     4021 ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt
     4081 tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt
     4141 caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg
     4201 ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg
     4261 cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt
     4321 gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc
     4381 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     4441 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
     4501 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     4561 taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc
     4621 taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     4681 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     4741 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4801 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4861 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat
     4921 gataagctgt caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt
     4981 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     5041 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5101 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5161 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5221 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5281 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5341 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     5401 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5461 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5521 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5581 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5641 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     5701 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5761 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5821 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5881 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5941 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6001 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6061 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6121 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6181 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6241 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6301 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6361 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6421 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6481 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6541 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6601 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6661 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6721 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6781 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6841 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6901 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6961 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     7021 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     7081 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     7141 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga
     7201 ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca
     7261 ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa
     7321 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     7381 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
     7441 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     7501 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     7561 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     7621 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     7681 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
     7741 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
     7801 gtattagtca tcgctattac catg
//
