LOCUS       sequence-234143-        6927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_105220-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2085..2102
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2154..2209
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2208..2227)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4445..4463)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4531..4635
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4636..4704,4705..5496)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4854..4873)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5667..6255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6156..6175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6409..6426
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6543..6564
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6579..6584,6585..6602,6603..6609)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6617..6633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6622..6644
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6641..6657
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6641..6657
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccatcaaga
     1201 acaagaaagt gaagtgctac cggtgcagcg actgccccaa ccccttcgac aagacccaga
     1261 tcaccgagct gggcaactgc aacttctgcc ggaccgtgta cacctaccgg gacgaggaca
     1321 actaccggat cgccaaggac tgcgtggcca gctgcgtgcc ccaggacaga agaggcggaa
     1381 aggccggcct ggtcaccgag tgctgcgacg aggactactg caacgccagc cctggcgcgc
     1441 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac
     1501 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt
     1561 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta
     1621 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg
     1681 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt
     1741 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct
     1801 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg
     1861 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt
     1921 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat
     1981 ccaaagggtt gaattccggc agcctgcacc acatcctgga cgcccagaag atgctgtgga
     2041 accacaggga caggaacctg cctcctctgg cccctctggg cccccaccac catcaccacc
     2101 attgagtcta gacacgtgat taattaagga tcccccgacc tcgacctctg gctaataaag
     2161 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     2221 atatgggagg gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat
     2281 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     2341 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     2401 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     2461 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     2521 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     2581 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     2641 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     2701 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     2761 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     2821 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     2881 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     2941 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     3001 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     3061 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     3121 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     3181 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     3241 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     3301 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     3361 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     3421 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     3481 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     3541 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     3601 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     3661 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     3721 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     3781 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     3841 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     3901 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     3961 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4021 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     4081 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     4141 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4201 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4261 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     4321 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     4381 cgggtagcat atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga
     4441 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4501 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4561 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4621 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4681 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4741 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4801 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4861 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     4921 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4981 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5041 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5101 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5161 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5221 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5281 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5341 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5401 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5461 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5521 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5581 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5641 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5701 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5761 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5821 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5881 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5941 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6001 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6061 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6121 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6181 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6241 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6301 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6361 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6421 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     6481 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     6541 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     6601 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     6661 gattacgcca agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc
     6721 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
     6781 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
     6841 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     6901 gctcatgtcc aatatgaccg ccatgtt
//
