LOCUS       sequence-234146-        7356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_031880-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2514..2531
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2583..2638
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2637..2656)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4874..4892)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4960..5064
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5065..5133,5134..5925)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5283..5302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6096..6684
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6585..6604
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6838..6855
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6972..6993
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7008..7013,7014..7031,7032..7038)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7046..7062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7051..7073
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7070..7086
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7070..7086
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccagaatcg
     1201 attgcggcag cagcgtgaag ctggaagtgg acttcgacaa cgtgcacagc cacggcatca
     1261 gctaccggaa ggccaccgtg aaaaagacca accccgtgaa cctggtgttc aacgtgatcg
     1321 gcgagcggag gcccatcgag atcgagagca tcgactggac ctacaacaac gtgtccatca
     1381 acatcggcga caacgacatc caggacatct actacagcac cgagaaaacc agcagcggcc
     1441 tgaccgtgac cctgcacatc aaggtgccca ccagcagagt ggccggcacc tggatcctga
     1501 ccatcaacgc caaggacaac agcaagcacg tgggcttttg ctacgtgtcc tgccagcccg
     1561 tgatccggtc cagatttggc gccgtgcggg gccacgaggg caaccctctg agcgtgctgt
     1621 gcgaggtgga cggcttcccc gagcccaacc aggtcgtgtg gaagcgggtg gtggaaaacg
     1681 aggccgaccc cacccagagc agactggtgc ccgtgcggaa caccgtgttc aagccccacg
     1741 agagcaccaa ggacgccatc atggaatgga acgacgccag cgacagcacc ggcttctacc
     1801 tgtgcaccgc caccagcccc ctgggcagcg acagcctgct gttcgaagtg cggatcaagt
     1861 ctggcgcgcc gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct
     1921 ccttcccact caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga
     1981 aggctccttc ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc
     2041 agaagtctac tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga
     2101 taccccaggt ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag
     2161 ggatactgta tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca
     2221 ctttgacctg tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg
     2281 agaatcagga ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag
     2341 caggggtgtg gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc
     2401 aggttttatc caaagggttg aattccggca gcctgcacca catcctggac gcccagaaga
     2461 tgctgtggaa ccacagggac aggaacctgc ctcctctggc ccctctgggc ccccaccacc
     2521 atcaccacca ttgagtctag acacgtgatt aattaaggat cccccgacct cgacctctgg
     2581 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     2641 cggaaggaca tatgggaggg caaatcattt ggtcgagatc cctcggagat ctctagctag
     2701 aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg
     2761 cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc
     2821 cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct
     2881 tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc
     2941 agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca
     3001 ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa
     3061 cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa
     3121 tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta
     3181 gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat
     3241 gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga
     3301 gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt
     3361 cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg
     3421 cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg
     3481 tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg
     3541 gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa
     3601 taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt
     3661 ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac
     3721 ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga
     3781 caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca
     3841 gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa
     3901 tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca
     3961 cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact
     4021 tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat
     4081 ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct
     4141 gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt
     4201 cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata
     4261 ctacccaaat atctggatag catatgctat cctaatctat atctgggtag cataggctat
     4321 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     4381 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     4441 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
     4501 cctaatagag attagggtag tatatgctat cctaatttat atctgggtag catatactac
     4561 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata tgctatccta
     4621 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     4681 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta
     4741 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     4801 atctgtatcc gggtagcata tgctatcctc atgataagct gtcaaacatg agaattttct
     4861 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4921 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4981 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5041 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5101 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5161 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5221 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5281 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     5341 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5401 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5461 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5521 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5581 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     5641 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5701 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5761 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5821 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5881 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5941 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6001 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6061 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6121 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6181 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6241 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6301 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6361 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6421 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6481 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6541 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6601 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6661 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6721 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6781 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6841 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6901 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     6961 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7021 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7081 tatgaccatg attacgccaa gctctagcta gaggtcgacc aattctcatg tttgacagct
     7141 tatcatcgca gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat
     7201 ggaagatcta tacattgaat caatattggc aattagccat attagtcatt ggttatatag
     7261 cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca
     7321 tttatattgg ctcatgtcca atatgaccgc catgtt
//
