LOCUS       sequence-234147-        6751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_009830-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2235..2252
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2304..2359
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2358..2377)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4269..4287)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4355..4459
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4460..4528,4529..5320)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4678..4697)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5491..6079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5980..5999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6233..6250
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6367..6388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6403..6408,6409..6426,6427..6433)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6441..6457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6446..6468
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6465..6481
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6465..6481
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccgatagcg
     1201 tggacaatgt gcggctggcc gtgtccaaag agatcctgaa cgagttcatt tttcaggaaa
     1261 aagagatgac catcctgtac accatctaca acttccacga gagcagagcc gccaaggacg
     1321 tggaactgtt cgacatcttc agcgagacag agttcaccct gatccacggc agccccagcg
     1381 ccagatggcc tgtggtgcct gccagcagca acgtgaccca cgtgatcatc ctgaagcccc
     1441 agatcaacgg cgtgcacaac ttcagcagcg ccaccatcac ctacaagagc accgacctgc
     1501 agaagtccag cttcctgtac agcagcgccc ctggcctgct gaccatccac cggctgaaag
     1561 agtacaacaa gcggttcacc tctggcgcgc cgtcgacctc catcacggcc tataagagtg
     1621 agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc ctgcagggag
     1681 agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc ttctccctaa
     1741 agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag ctgtccgaaa
     1801 cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt tctggcaacc
     1861 tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg gtgatgaaag
     1921 tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc tcacccaaga
     1981 tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag gagaaagtga
     2041 ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa ggtgaagagg
     2101 tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccggc agcctgcacc
     2161 acatcctgga cgcccagaag atgctgtgga accacaggga caggaacctg cctcctctgg
     2221 cccctctggg cccccaccac catcaccacc attgagtcta gacacgtgat taattaagga
     2281 tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     2341 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat
     2401 ccctcggaga tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     2461 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     2521 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     2581 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     2641 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     2701 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     2761 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     2821 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     2881 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     2941 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     3001 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     3061 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     3121 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     3181 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     3241 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     3301 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     3361 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     3421 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     3481 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     3541 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     3601 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     3661 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     3721 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     3781 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     3841 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     3901 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     3961 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     4021 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4081 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     4141 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     4201 tatccgggta gcatatgcta tcctcatgat aagctgtcaa acatgagaat tttcttgaag
     4261 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4321 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4381 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4441 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4501 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4561 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4621 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4681 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     4741 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4801 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4861 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4921 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4981 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5041 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5101 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5161 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5221 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5281 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5341 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5401 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5461 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5521 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5581 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5641 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5701 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5761 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5821 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5881 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5941 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6001 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6061 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6121 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6181 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6241 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6301 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     6361 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     6421 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     6481 ccatgattac gccaagctct agctagaggt cgaccaattc tcatgtttga cagcttatca
     6541 tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag
     6601 atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa
     6661 atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat
     6721 attggctcat gtccaatatg accgccatgt t
//
