LOCUS       sequence-234148-        7329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_074000-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2487..2504
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2556..2611
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2610..2629)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4847..4865)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4933..5037
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5038..5106,5107..5898)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5256..5275)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6069..6657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6558..6577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6811..6828
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6945..6966
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6981..6986,6987..7004,7005..7011)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7019..7035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7024..7046
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7043..7059
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7043..7059
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccattgagc
     1201 tgcccctgac ccccaagacc tgcctggacg tgtgcgtgac caagttcgac ggcgccgaag
     1261 aggacgcctg cagcagaggc tgcgtgctga gcaacctgaa cagcctgccc gccaccttca
     1321 cctgtgaaga gtcctgcgac gacgtgtaca agcccgacac caagctggtg gccgcctgca
     1381 aagtgggctg tggcgccacc cctagccaca ccagcatccg gctgggcttt ctgcccgtgc
     1441 agaacttctt cagcagcttc ttcgacagca tcagacatct gctgggcggc gtgaacaacg
     1501 gcagcggcgc caacgccgat accaagacca acaaggtgga cggcgacatg gacagcgaca
     1561 gcggcaacca cgtggtcatc agcagagtgc ggatcttcca caacttcgcc ccctacgacg
     1621 cccacgagag ccccctggac agccccttcg acatcaagcc cctgttcagc ctgcggggca
     1681 tcatccccca ggacgacgac gcctacgtga aggccgtgaa gaacagcgac cagaacatca
     1741 tccggatcca cgccgtgtcc ggcgacggcg agagcacctt tcacgagcac cagcccacct
     1801 tcgtgcggaa ggccacctac tttttccggc acattggcgc gccgtcgacc tccatcacgg
     1861 cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt ggagaggaaa
     1921 gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag tcctggatca
     1981 ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac cccaagttcc
     2041 agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt cagtttgctg
     2101 gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa gtgaacctgg
     2161 tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg atgggaccca
     2221 cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg gtctccaggc
     2281 aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt ctactgagtg
     2341 aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg ttgaattccg
     2401 gcagcctgca ccacatcctg gacgcccaga agatgctgtg gaaccacagg gacaggaacc
     2461 tgcctcctct ggcccctctg ggcccccacc accatcacca ccattgagtc tagacacgtg
     2521 attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta ttttcattgc
     2581 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     2641 tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc cggacgaact
     2701 aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt
     2761 tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac
     2821 acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac
     2881 actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc
     2941 ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca
     3001 ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta
     3061 ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac
     3121 cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat
     3181 tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg
     3241 tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt
     3301 caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg
     3361 agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa
     3421 tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt
     3481 caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga
     3541 caccaatata accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt
     3601 ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc
     3661 catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg
     3721 gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat
     3781 ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa
     3841 cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc
     3901 cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc
     3961 ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact
     4021 gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt
     4081 aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac
     4141 ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc
     4201 acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc
     4261 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     4321 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     4381 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     4441 tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc
     4501 tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc
     4561 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc
     4621 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     4681 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     4741 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
     4801 ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct cgtgatacgc
     4861 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     4921 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4981 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5041 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5101 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5161 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5221 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5281 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5341 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5401 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5461 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5521 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5581 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5641 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5701 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5761 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5821 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5881 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5941 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6001 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6061 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6121 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6181 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6241 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6301 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6361 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6421 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6481 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6541 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6601 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6661 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6721 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     6781 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     6841 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     6901 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     6961 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     7021 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag
     7081 ctagaggtcg accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt
     7141 tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt
     7201 ggcaattagc catattagtc attggttata tagcataaat caatattggc tattggccat
     7261 tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac
     7321 cgccatgtt
//
