LOCUS       sequence-234154-        6939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_124000-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1717..1734
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1786..1841
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1840..1859)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4077..4095)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4163..4267
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4268..4336,4337..5128)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4486..4505)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5299..5887
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5788..5807
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6041..6058
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6175..6196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6211..6216,6217..6234,6235..6241)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6249..6265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6254..6276
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6273..6289
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6273..6289
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6560..6939
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccacaagcg ccaatagcag aacccacggc
      841 gccaccagca ccagcaccca tggcgccaca tctaccgcca agcctgccgc cagcacccct
      901 cctaaggccg ctgccacctc caccatcaag cccaccgtga ccacccccaa ggccgcagcc
      961 acaagcacaa tcaaacctac agtgaccacc aagcccagcc ccgccaagcc cgctgcctcc
     1021 aatactgcca aaccagccgc ctccaccccc aaaaagcccc acgatggcgc gccgtcgacc
     1081 tccatcacgg cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt
     1141 ggagaggaaa gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag
     1201 tcctggatca ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac
     1261 cccaagttcc agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt
     1321 cagtttgctg gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa
     1381 gtgaacctgg tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg
     1441 atgggaccca cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg
     1501 gtctccaggc aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt
     1561 ctactgagtg aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg
     1621 ttgaattccg gcagcctgca ccacatcctg gacgcccaga agatgctgtg gaaccacagg
     1681 gacaggaacc tgcctcctct ggcccctctg ggcccccacc accatcacca ccattgagtc
     1741 tagacacgtg attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta
     1801 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     1861 gggcaaatca tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc
     1921 cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa
     1981 tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg
     2041 gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac
     2101 atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac
     2161 acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat
     2221 gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc
     2281 tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc
     2341 ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac
     2401 taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt
     2461 agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt
     2521 tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg
     2581 aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc
     2641 gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa
     2701 aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat
     2761 tgtgctatga caccaatata accctcacaa accccttggg caataaatac tagtgtagga
     2821 atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag
     2881 actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata
     2941 tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt
     3001 tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg
     3061 ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa
     3121 gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac
     3181 gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc
     3241 gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac
     3301 ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa
     3361 ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt
     3421 cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga
     3481 tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg
     3541 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg
     3601 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     3661 tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg
     3721 tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc
     3781 atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc
     3841 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     3901 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     3961 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     4021 atatgctatc ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct
     4081 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     4141 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4201 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4261 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4321 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4381 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4441 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4501 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4561 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4621 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4681 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     4741 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     4801 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     4861 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     4921 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4981 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5041 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5101 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5161 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5221 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5281 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5341 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5401 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     5461 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5521 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5581 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5641 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5701 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5761 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5821 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5881 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5941 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6001 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6061 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6121 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6181 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6241 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     6301 caagctctag ctagaggtcg accaattctc atgtttgaca gcttatcatc gcagatccgg
     6361 gcaacgttgt tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg
     6421 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc
     6481 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
     6541 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
     6601 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     6661 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     6721 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     6781 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat
     6841 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact
     6901 tggcagtaca tctacgtatt agtcatcgct attaccatg
//
