LOCUS       sequence-234156-        7464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_174580-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2622..2639
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2691..2746
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2745..2764)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4982..5000)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5068..5172
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5173..5241,5242..6033)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5391..5410)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6204..6792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6693..6712
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6946..6963
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7080..7101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7116..7121,7122..7139,7140..7146)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7154..7170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7159..7181
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7178..7194
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7178..7194
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccagctacc
     1201 acatccggga caacagcctg gtgcccccct acagcagccc ccactgggtg ccatacggca
     1261 gcaccgtgat cgagccccag ttcgtgcggc tgaccagcga catcaagagc agccagggcg
     1321 gcatcttcaa catcaagccc ctgttcgccc gggactggga ggtggtcatc ctgttccacg
     1381 tgcacagccc caagaccctc gtgggcgacg gcttcgcctt ctggtacacc cagcaccccc
     1441 ctagcagcgg ccctgccttc ggcagcagag acaagttccg gggcctggcc gtgttcttcg
     1501 acacctacgc caaccagaac ggcgagcaca gccacgagca cccctacgtg tccgccatga
     1561 tcaacgacgg cagcaagagc tacgaccacg acaaggacgg caccctgacc gagctgagcg
     1621 gctgcagcag caacttccgg aacaacgact acagcatggc caccatcaga tacgccaaca
     1681 accagctgaa ggtgtccctg aagtaccagg gcgctgccga ccccgtggac tgcttcaccg
     1741 tggacggcgt gcggctgccc accggctact ttatcggagt gtccgccgcc accggcgacc
     1801 tgagcgacaa ccacgacatc tacagcatcc acacctacga gctggacgtg ggccggaagg
     1861 acgacccctt cgccgacttc agcaagatcg agcccagcgc cgaccaggaa gccgccccta
     1921 gagccagagt ggacgacacc ccccccagcc ggttcagcag agtgatcaca ggcgcgccgt
     1981 cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc ttcccactca
     2041 accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag gctccttctt
     2101 cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag aagtctacta
     2161 gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata ccccaggtct
     2221 cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg atactgtatc
     2281 aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact ttgacctgtg
     2341 aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag aatcaggagg
     2401 ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc
     2461 aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag gttttatcca
     2521 aagggttgaa ttccggcagc ctgcaccaca tcctggacgc ccagaagatg ctgtggaacc
     2581 acagggacag gaacctgcct cctctggccc ctctgggccc ccaccaccat caccaccatt
     2641 gagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct aataaaggaa
     2701 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     2761 tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc
     2821 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     2881 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     2941 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     3001 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     3061 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     3121 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     3181 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     3241 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     3301 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     3361 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     3421 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     3481 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     3541 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     3601 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     3661 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     3721 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     3781 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     3841 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     3901 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     3961 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     4021 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     4081 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     4141 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     4201 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     4261 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     4321 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     4381 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     4441 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     4501 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4561 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
     4621 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
     4681 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     4741 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     4801 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     4861 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     4921 gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg aagacgaaag
     4981 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5041 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5101 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5161 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5221 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5281 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5341 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5401 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5461 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5521 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5581 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5641 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5701 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5761 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5821 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5881 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5941 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6001 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6061 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6121 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6181 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6241 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6301 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6361 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6421 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6481 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6541 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6601 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6661 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6721 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6781 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6841 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6901 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6961 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7021 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     7081 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     7141 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     7201 tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     7261 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata
     7321 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
     7381 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
     7441 catgtccaat atgaccgcca tgtt
//
