LOCUS       sequence-234157-       12291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12291)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 12291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-MAPKAP1.1-FHH-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..12291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     enhancer        2791..3094
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3095..3298
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3249..3269
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3295..3319
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4956..4979
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4986..5012
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5022..5039
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    5082..5654
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5249..5266)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5476..5495
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5657..6259
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6156..6176
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6266..6854
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6319..6339)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6737..6748)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7061..7294
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7395..7412)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7401..7625
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7671..8099
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7758..7777)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7968..7989
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8108..8128)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8113..8442
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8293..8428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8355..8374
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8491..9516
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8491..9516
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    9646..9767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9816..9832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9816..9832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9829..9851)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9840..9856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9864..9870,9871..9888,9889..9894))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9909..9930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10047..10064)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10218..10806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10298..10317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10977..11768,11769..11837))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11600..11619
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11838..11942)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11990..12124
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
     2761 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2821 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2881 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2941 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     3001 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     3061 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     3121 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     3181 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     3241 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     3301 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     3361 acaccgactc tactagagga tccatggcct tcttggacaa tccaactatc attctagctc
     3421 atattcgaca gtcacatgtg accagtgatg acacgggaat gtgtgagatg gttctcattg
     3481 atcatgatgt tgacctagag aagattcatc ctccttcaat gcctggagac agtgggtcag
     3541 aaattcaggg aagcaatggt gagactcagg gctatgtata tgcccagtca gtcgatatta
     3601 cctcaagttg ggactttggt attagaagac gctcaaacac agctcaaaga ttagaacgac
     3661 tccgaaaaga gagacaaaac cagatcaaat gcaaaaatat tcagtggaaa gaaagaaatt
     3721 ctaagcaatc agcccaggag ttaaagtcac tgtttgaaaa aaaatctctc aaagagaagc
     3781 ctccaatttc tgggaagcag tcgatattat ctgtacgcct agaacagtgc cctctgcagc
     3841 tgaataaccc ttttaacgag tattccaaat ttgatggcaa gggtcatgta ggtacaacag
     3901 caaccaagaa gatcgatgtc tacctccctc tgcactcgag ccaggacaga ctgctgccaa
     3961 tgaccgtggt gacaatggcc agcgccaggg tgcaggacct gatcgggctc atctgctggc
     4021 agtatacaag cgaaggacgg gagccgaagc tcaatgacaa tgtcagtgcc tactgcctgc
     4081 atattgctga ggatgatggg gaggtggaca ccgatttccc cccgctggat tccaatgagc
     4141 ccattcataa gtttggcttc agtactttgg ccctggttga aaagtactca tctcctggtc
     4201 tgacatccaa agagtcactc tttgttcgaa taaatgctgc tcatggattc tcccttattc
     4261 aggtggacaa cacaaaggtt accatgaagg aaatcttact gaaggcagtg aagcgaagaa
     4321 aaggatccca gaaagtttca ggccctcagt accgcctgga gaagcagagc gagcccaatg
     4381 tcgccgttga cctggacagc actttggaga gccagagcgc atgggagttc tgcctggtcc
     4441 gcgagaacag ttcaagggca gacggggttt ttgaggagga ttcgcaaatt gacatagcca
     4501 cagtacagga tatgcttagc agccaccatt acaagtcatt caaagtcagc atgatccaca
     4561 gactgcgatt cacaaccgac gtacagctag gtatctctgg agacaaagta gagatagacc
     4621 ctgttacgaa tcagaaagcc agcactaagt tttggattaa gcagaaaccc atctcaatcg
     4681 attccgacct gctctgtgcc tgtgaccttg ctgaagagaa aagccccagt cacgcaatat
     4741 ttaaactcac gtatctaagc aatcacgact ataaacacct ctactttgaa tcggacgctg
     4801 ctaccgtcaa tgaaattgtg ctcaaggtta actacatcct ggaatcgcga gctagcactg
     4861 cccgggctga ctactttgct caaaaacaaa gaaaactgaa cagacgtacg agcttcagct
     4921 tccagaagga gaagaaatcc gggcagcagc tcgaggatta taaagatgac gacgataaag
     4981 caggttaccc atacgatgtg cctgactatg ctgcaggttc acatcatcac caccatcatt
     5041 gagtcgagtc tagagggccc gacgcgtctg gccaccggtc gcccctctcc ctcccccccc
     5101 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     5161 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     5221 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     5281 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     5341 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     5401 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     5461 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     5521 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     5581 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     5641 aaaacacgat aataccatgg ccaccgagta caagcccacg gtgcgcctcg ccacccgcga
     5701 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     5761 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     5821 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     5881 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggctcgcg
     5941 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     6001 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     6061 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     6121 tggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     6181 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     6241 ccgcaagccc ggtgcctgag ttaacaatca acctctggat tacaaaattt gtgaaagatt
     6301 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6361 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6421 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6481 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6541 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6601 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6661 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6721 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6781 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6841 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc
     6901 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     6961 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     7021 gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc
     7081 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7141 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7201 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7261 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7321 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgttta
     7381 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     7441 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     7501 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     7561 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     7621 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     7681 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7741 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7801 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7861 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7921 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7981 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     8041 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     8101 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     8161 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     8221 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     8281 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8341 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     8401 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     8461 atatccattt tcggatctga tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct
     8521 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     8581 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     8641 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     8701 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     8761 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     8821 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     8881 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     8941 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     9001 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     9061 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     9121 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     9181 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     9241 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     9301 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     9361 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     9421 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     9481 cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tgctacgaga tttcgattcc
     9541 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     9601 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     9661 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9721 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     9781 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9841 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     9901 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     9961 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    10021 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10081 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10141 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10201 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10261 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    10321 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10381 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    10441 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    10501 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10561 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10621 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    10681 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    10741 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10801 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10861 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10921 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10981 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    11041 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    11101 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    11161 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    11221 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    11281 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    11341 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    11401 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    11461 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    11521 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    11581 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    11641 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    11701 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    11761 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    11821 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    11881 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    11941 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat
    12001 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    12061 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    12121 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    12181 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    12241 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
