LOCUS       sequence-234168-        7911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_060570-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2689..2706
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2758..2813
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2812..2831)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5049..5067)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5135..5239
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5240..5308,5309..6100)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5458..5477)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6271..6859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6760..6779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7013..7030
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7147..7168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7183..7188,7189..7206,7207..7213)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7221..7237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7226..7248
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7245..7261
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7245..7261
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7532..7911
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gcctacaacc ccatgagcga gaagctggaa
      841 agcctgctgc tgagcctgga caagctggtg tactactacg agcagcacgt gtccgagttc
      901 aacttcgacg gcatcttcgg cctgatcccc ctgaagggcg ccctggaatc catcaccgac
      961 gacagcctgt acgagaacta cgagagagtg aagatcgccc tgctgcccct gatgcggaag
     1021 ctgaaaaccc agatcatgcg gatcaacgtg atcatccgga agtccgtgaa gttcttcaag
     1081 aaggcccggg acagctacta caccaagatg ggccggctgc tggccctgaa gtggaacttc
     1141 cccggccaga acaccatgta cagctacctg agcgaccccg agcccatcag cagcgcccag
     1201 aacatcagct ccgacgagtg catcatccag ctgctgaaca ccaacaacta cggcaccctg
     1261 gactgccacc tgagcatgga atgcatcctg aagctgatgg acttcaacag ccggggctac
     1321 gagctggccc accaggtgct gtacatcctg atcagccacc aggtcaactg caccgacagc
     1381 ctggacggca tgctgagcaa cctgagcacc agcgtgggca gcctgcagaa caagctgtgc
     1441 agcatcctgt accgggactt cgtgaacctg ttcctgagca gcgagacaac cccccggaac
     1501 cgggacatcc tgctggaaga gatgttcgtg tgcggcagca tcggctacga gaacttcatc
     1561 cagttcagca tcctgagccg ggtgctgagc tggcagcagc ccagcggctg cttcatctcc
     1621 agcggcagca ccgacggcaa gagcaagcac aagaacacca agatcatgtt ccacgagcgg
     1681 caccccctgg tggaacacag acacgccgat ggctgcctga gccacatgac ctctgtggcc
     1741 gctgccgtga tgggcctgta cttcagagcc ttcttcatcc ccaccggcta cgtggacggc
     1801 tacctgtccg ccagcatgcc tattatctgg aaggtgttcc tcgtgcggag ctacaccagc
     1861 ctgcccaagc tgatcgagga cgacaacaac caggacggca acagcaacca ggacatcttc
     1921 aacgccttca ccaaccggct gatccacgtg aagggcagca cctacctgcc cagcggcatc
     1981 cccttcagcc tgagctacag cacccacttc aaggataagc tggacctgat caactctggc
     2041 gcgccgtcga cctccatcac ggcctataag agtgaggggg agtcagcgga gttctccttc
     2101 ccactcaacc ttggagagga aagcctgcag ggagagttga gatggaaggc agagaaggct
     2161 ccttcttccc agtcctggat caccttctcc ctaaagaacc aaaaggtgtc tgtgcagaag
     2221 tctactagca accccaagtt ccagctgtcc gaaacgctcc cactcaccct tcagataccc
     2281 caggtctccc ttcagtttgc tggttctggc aacctgaccc tgactctgga cagagggata
     2341 ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc agcccgacag caacactttg
     2401 acctgtgagg tgatgggacc cacctcaccc aagatgagac tgatcttgaa gcaggagaat
     2461 caggaggcca gggtctccag gcaggagaaa gtgattcaag tgcaggcccc tgaagcaggg
     2521 gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga tggactccaa gatccaggtt
     2581 ttatccaaag ggttgaattc cggcagcctg caccacatcc tggacgccca gaagatgctg
     2641 tggaaccaca gggacaggaa cctgcctcct ctggcccctc tgggccccca ccaccatcac
     2701 caccattgag tctagacacg tgattaatta aggatccccc gacctcgacc tctggctaat
     2761 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     2821 ggacatatgg gagggcaaat catttggtcg agatccctcg gagatctcta gctagaggat
     2881 cgatccccgc cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg
     2941 cagtgcatgt aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat
     3001 gtgacacggg gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa
     3061 atggatgtgc acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct
     3121 gtggactgca acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat
     3181 gctgggggac atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca
     3241 atcagagggg cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg
     3301 tttataaggc ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata
     3361 tactatccag actaacccta attcaatagc atatgttacc caacgggaag catatgctat
     3421 cgaattaggg ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt
     3481 cacggtttta tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa
     3541 gggcagtggc tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct
     3601 tcattctcct tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt
     3661 gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt
     3721 cagtggtggc attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat
     3781 actagtgtag gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga
     3841 caagccgtaa agactggatg tccatctcac acgaatttat ggctatgggc aacacataat
     3901 cctagtgcaa tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt
     3961 gaaccatgtt gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc
     4021 ggactccact ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg
     4081 cccataaaca aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt
     4141 cagcccccac acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct
     4201 gattgtaacc ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac
     4261 cccggggaat acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat
     4321 ctggaggaca aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca
     4381 tattcacgag gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc
     4441 caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa
     4501 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     4561 tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     4621 tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa
     4681 tagagattag ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa
     4741 tatctggata gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4801 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4861 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     4921 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     4981 tatccgggta gcatatgcta tcctcatgat aagctgtcaa acatgagaat tttcttgaag
     5041 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     5101 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5161 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5221 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5281 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5341 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5401 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5461 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     5521 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5581 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5641 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5701 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5761 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5821 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5881 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5941 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6001 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6061 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6121 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6181 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6241 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6301 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6361 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6421 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6481 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6541 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6601 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6661 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6721 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6781 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6841 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6901 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6961 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7021 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     7081 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     7141 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     7201 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     7261 ccatgattac gccaagctct agctagaggt cgaccaattc tcatgtttga cagcttatca
     7321 tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag
     7381 atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa
     7441 atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat
     7501 attggctcat gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag
     7561 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     7621 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     7681 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     7741 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct
     7801 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg
     7861 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat g
//
