LOCUS       sequence-234169-        7299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_075280-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2077..2094
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2146..2201
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2200..2219)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4437..4455)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4523..4627
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4628..4696,4697..5488)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4846..4865)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5659..6247
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6148..6167
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6401..6418
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6535..6556
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6571..6576,6577..6594,6595..6601)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6609..6625
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6614..6636
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6633..6649
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6633..6649
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6920..7299
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccgtgcgga acacccagtt catcacccag
      841 cacgacccct tcgccaccac cgtgtccccc gtgatcacca acgtgtccca gaagtccagc
      901 aacaaatacg agatcctgct gagcagcgac tgcgtggcca tccggtccat catcaacatc
      961 accgtggaag ataaggacaa caacaccagc agcaccatct acctgaacaa gaccctgcag
     1021 aacaagatca tcgtgaacag cacatgcgag cagaagcggc aggacctgag cgtgtcctgg
     1081 tcccccaacg tgaacctgag caactggaag ctgatcttcg tgttcggcga ggacagcgag
     1141 agatacgacc tggaacggat cggcgtgacc tacaccacca tcaagggcaa cgagatcgac
     1201 gccaacaccg accggggcct gttcaccatc cccgtgggcg gctactacga gtgcgccagc
     1261 cggctgcaca gagagctgtt cgtcgaggat aacgacaaca ttatgatcaa tatctacttc
     1321 ctgaacagca gcatggaagc cttccggctg gaaaaccggc gcgagttcat gggccacgcc
     1381 atcgactgcc ccctgagcat cctgtggctg aagaaggtgc ccacaggcgc gccgtcgacc
     1441 tccatcacgg cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt
     1501 ggagaggaaa gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag
     1561 tcctggatca ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac
     1621 cccaagttcc agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt
     1681 cagtttgctg gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa
     1741 gtgaacctgg tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg
     1801 atgggaccca cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg
     1861 gtctccaggc aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt
     1921 ctactgagtg aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg
     1981 ttgaattccg gcagcctgca ccacatcctg gacgcccaga agatgctgtg gaaccacagg
     2041 gacaggaacc tgcctcctct ggcccctctg ggcccccacc accatcacca ccattgagtc
     2101 tagacacgtg attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta
     2161 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     2221 gggcaaatca tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc
     2281 cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa
     2341 tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg
     2401 gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac
     2461 atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac
     2521 acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat
     2581 gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc
     2641 tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc
     2701 ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac
     2761 taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt
     2821 agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt
     2881 tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg
     2941 aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc
     3001 gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa
     3061 aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat
     3121 tgtgctatga caccaatata accctcacaa accccttggg caataaatac tagtgtagga
     3181 atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag
     3241 actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata
     3301 tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt
     3361 tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg
     3421 ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa
     3481 gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac
     3541 gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc
     3601 gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac
     3661 ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa
     3721 ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt
     3781 cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga
     3841 tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg
     3901 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg
     3961 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     4021 tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg
     4081 tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc
     4141 atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc
     4201 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     4261 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     4321 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     4381 atatgctatc ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct
     4441 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     4501 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4561 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4621 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4681 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4741 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4801 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4861 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4921 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4981 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5041 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5101 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5161 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5221 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5281 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5341 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5401 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5461 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5521 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5581 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5641 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5701 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5761 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     5821 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5881 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5941 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6001 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6061 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6121 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6181 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6241 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6301 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6361 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6421 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6481 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6541 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6601 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     6661 caagctctag ctagaggtcg accaattctc atgtttgaca gcttatcatc gcagatccgg
     6721 gcaacgttgt tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg
     6781 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc
     6841 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
     6901 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
     6961 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     7021 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     7081 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     7141 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat
     7201 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact
     7261 tggcagtaca tctacgtatt agtcatcgct attaccatg
//
