LOCUS       sequence-234189-       12480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-Mtor Kinase Domain fusion protein in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12480)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 12480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-MTORKinaseDomain
FEATURES             Location/Qualifiers
     source          1..12480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     enhancer        2791..3094
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3095..3298
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3249..3269
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3295..3319
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3390..3413
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3420..3446
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3456..3473
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3642..3920
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             3654..3926
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
     misc_feature    5271..5843
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5438..5455)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5665..5684
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5846..6448
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6345..6365
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6455..7043
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6508..6528)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6926..6937)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7250..7483
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7584..7601)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7590..7814
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7860..8288
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7947..7966)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8157..8178
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8297..8317)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8302..8631
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8482..8617
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8544..8563
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8680..9705
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8680..9705
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    9835..9956
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10005..10021)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10005..10021)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10018..10040)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10029..10045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10053..10059,10060..10077,10078..10083))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10098..10119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10236..10253)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10407..10995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10487..10506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11166..11957,11958..12026))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11789..11808
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12027..12131)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12179..12313
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
     2761 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2821 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2881 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2941 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     3001 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     3061 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     3121 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     3181 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     3241 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     3301 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     3361 acaccgactc tactagagga tctaccatgg attataaaga tgacgacgat aaagcaggtt
     3421 acccatacga tgtgcctgac tatgctgcag gttcacatca tcaccaccat catggatcct
     3481 accaccccca ggccctcatc tacccactga cagtggcttc taagtctacc acgacagccc
     3541 ggcacaatgc agccaacaag attctgaaga acatgtgtga gcacagcaac accctggtcc
     3601 agcaggccat gatggtgagc gaggagctga tccgagtggc catcctctgg catgagatgt
     3661 ggcatgaagg cctggaagag gcatctcgtt tgtactttgg ggaaaggaac gtgaaaggca
     3721 tgtttgaggt gctggagccc ttgcatgcta tgatggaacg gggcccccag actctgaagg
     3781 aaacatcctt taatcaggcc tatggtcgag atttaatgga ggcccaagag tggtgcagga
     3841 agtacatgaa atcagggaat gtcaaggacc tcacccaagc ctgggacctc tattatcatg
     3901 tgttccgacg aatctcaaag cagctgcctc agctcacatc cttagagctg caatatgttt
     3961 ccccaaaact tcttatgtgc cgggaccttg aattggctgt gccaggaaca tatgacccca
     4021 accagccaat cattcgcatt cagtccatag caccgtcttt gcaagtcatc acatccaagc
     4081 agaggccccg gaaattgaca cttatgggca gcaacggaca tgagtttgtt ttccttctaa
     4141 aaggccatga agatctgcgc caggatgagc gtgtgatgca gctcttcggc ctggttaaca
     4201 cccttctggc caatgaccca acatctcttc ggaaaaacct cagcatccag agatacgctg
     4261 tcatcccttt atcgaccaac tcgggcctca ttggctgggt tccccactgt gacacactgc
     4321 acgccctcat ccgggactac agggagaaga agaagatcct tctcaacatc gagcatcgca
     4381 tcatgttgcg gatggctccg gactatgacc acttgactct gatgcagaag gtggaggtgt
     4441 ttgagcatgc cgtcaataat acagctgggg acgacctggc caagctgctg tggctgaaaa
     4501 gccccagctc cgaggtgtgg tttgaccgaa gaaccaatta tacccgttct ttagcggtca
     4561 tgtcaatggt tgggtatatt ttaggcctgg gagatagaca cccatccaac ctgatgctgg
     4621 accgtctgag tgggaagatc ctgcacattg actttgggga ctgctttgag gttgctatga
     4681 cccgagagaa gtttccagag aagattccat ttagactaac aagaatgttg accaatgcta
     4741 tggaggttac aggcctggat ggcaactaca gaatcacatg ccacacagtg atggaggtgc
     4801 tgcgagagca caaggacagt gtcatggccg tgctggaagc ctttgtctat gaccccttgc
     4861 tgaactggag gctgatggac acaaatacca aaggcaacaa gcgatcccga acgaggacgg
     4921 attcctactc tgctggccag tcagtcgaaa ttttggacgg tgtggaactt ggagagccag
     4981 cccataagaa aacggggacc acagtgccag aatctattca ttctttcatt ggagacggtt
     5041 tggtgaaacc agaggcccta aataagaaag ctatccagat tattaacagg gttcgagata
     5101 agctcactgg tcgggacttc tctcatgatg acactttgga tgttccaacg caagttgagc
     5161 tgctcatcaa acaagcgaca tcccatgaaa acctctgcca gtgctatatt ggctggtgcc
     5221 ctttctggta actcgagtct agagggcccg acgcgtctgg ccaccggtcg cccctctccc
     5281 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     5341 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     5401 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     5461 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     5521 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     5581 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     5641 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     5701 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     5761 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     5821 ttcctttgaa aaacacgata ataccatggc caccgagtac aagcccacgg tgcgcctcgc
     5881 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     5941 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga
     6001 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     6061 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     6121 cggctcgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     6181 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     6241 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     6301 cgagcgcgct ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     6361 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     6421 gtgcatgacc cgcaagcccg gtgcctgagt taacaatcaa cctctggatt acaaaatttg
     6481 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6541 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6601 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     6661 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     6721 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     6781 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6841 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     6901 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6961 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7021 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat
     7081 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     7141 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     7201 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta
     7261 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     7321 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7381 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7441 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7501 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga
     7561 cattgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     7621 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     7681 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     7741 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     7801 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     7861 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     7921 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     7981 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8041 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8101 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8161 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     8221 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     8281 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     8341 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     8401 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     8461 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8521 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     8581 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     8641 ggagcttgta tatccatttt cggatctgat cagcacgtga tgaaaaagcc tgaactcacc
     8701 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     8761 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     8821 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     8881 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
     8941 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     9001 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     9061 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     9121 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     9181 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     9241 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     9301 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     9361 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     9421 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     9481 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     9541 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     9601 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     9661 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt gctacgagat
     9721 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     9781 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     9841 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9901 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9961 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
    10021 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    10081 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    10141 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    10201 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    10261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    10321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    10381 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    10441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10741 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10801 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    10861 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10921 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10981 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    11041 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    11101 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    11161 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    11221 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    11281 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    11341 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    11401 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    11461 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    11521 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    11581 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    11641 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    11701 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    11761 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    11821 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    11881 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    11941 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    12001 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    12061 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    12121 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa
    12181 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    12241 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    12301 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
    12361 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
    12421 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
