LOCUS       sequence-234193-        8196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_178740-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1513..1522
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2974..2991
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3043..3098
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3097..3116)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5334..5352)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5420..5524
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5525..5593,5594..6385)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5743..5762)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6556..7144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7045..7064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7298..7315
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7432..7453
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7468..7473,7474..7491,7492..7498)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7506..7522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7511..7533
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7530..7546
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7530..7546
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7817..8196
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gcccagcctt gttctgatgc ccaggccgtg
      841 atccagcagc ggagccagaa cctgaagccc ctgaccacca tccggccctt cgtgcccaca
      901 gtgcccggca tggccatcaa caccaacacc gccgtgcagt acgtgcggaa gcccgccgag
      961 atctctgtgc tgggagccgc cggaaccttc gtggccaccc ccatgaacca gaccttcacc
     1021 cccttcgtga accccatcat gaacctgaac cccagcctga tcagcagcat ccctggcggc
     1081 cctacaggcg gccctggaca ggtgtcatct ctgagcgcca caggcggcgt gctgagcagc
     1141 ctggccaaca gcagaaagcc ctgcaactgc accaagagcc actgcctgaa gctgtactgc
     1201 gagtgcttcg cccagggcca gctgtgccag aactgcaatt gcaacaactg catgaacaac
     1261 ctggcctacg aggaagagag aggcagagcc atcaagatga ccctggaacg gaaccccacc
     1321 gccttccacc ccaagatcgg acggggcgag ggcgagagaa agcacaccaa gggctgtaac
     1381 tgcaagcgga gcggctgcct gaagaactat tgcgagtgct acgaggccaa gatcagctgc
     1441 agcgacctgt gccggtgcca gggctgcaga aacaccgagg acagcgtgga acggcggagc
     1501 ctgatgagac tggccgccat gggcgccctg agaagcagac agcccagcag cttcaagaag
     1561 cacctggtca agccccccag cgagacactg ccccacgtgt tcttcacctg ggaagtgatc
     1621 gaggccaccg ccagctgtct gctggcccag gccgatgagg ccgagagaag agatctgccc
     1681 cctgccgccc aggaacggat cctgctggaa gagttcggcc ggaccctgga aagagtgatt
     1741 gaggccgcca gcaaggccca gatcagaagc agcagcggca tccacaccag cgccagcatc
     1801 gacgacaacg accagggcct gatccactcc ggcggcagca caggccctgg atctctgggc
     1861 ggaaagggct ctggcgccgt gggagtggct gctgccgctg cagctgctgc cgacgtgctg
     1921 agggacgacg aggatgacga ggacgagatg atgcacgaca tcgacgtgga agaggaagag
     1981 gacgaccccg acgagatgga cgacgagatg tacggcatcg tgggcggacc caccatcagc
     2041 cggcacggcc acagcagaag ccacctgggc agacggcaca tccccagcag ccacagatcc
     2101 acccacctgg tgcccgagct ggaagatgac atcatgagcg acgaggaccc cgtggaaagg
     2161 gcccacgccg tgtcctccag caacagacag gccatcatgc cccacagata ccaggaaatg
     2221 cacagccggc ggaccagcca gaccgtggaa gatcggtaca tccacagcag gcggagcatg
     2281 agcagcagaa gctaccggca ccacgacccc gatagcatgg tcggcgcgcc gtcgacctcc
     2341 atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga
     2401 gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc
     2461 tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc
     2521 aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag
     2581 tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg
     2641 aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg
     2701 ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc
     2761 tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta
     2821 ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg
     2881 aattccggca gcctgcacca catcctggac gcccagaaga tgctgtggaa ccacagggac
     2941 aggaacctgc ctcctctggc ccctctgggc ccccaccacc atcaccacca ttgagtctag
     3001 acacgtgatt aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt
     3061 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     3121 caaatcattt ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg
     3181 acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc
     3241 cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg
     3301 accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt
     3361 tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca
     3421 acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta
     3481 cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt
     3541 gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct
     3601 tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa
     3661 ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt
     3721 aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac
     3781 atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag
     3841 atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt
     3901 tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac
     3961 aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt
     4021 gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg
     4081 aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact
     4141 ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga
     4201 tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac
     4261 actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg
     4321 tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac
     4381 aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc
     4441 gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct
     4501 aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg
     4561 cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta
     4621 cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc
     4681 tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag
     4741 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     4801 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     4861 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     4921 tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag attagggtag
     4981 tatatgctat cctaatttat atctgggtag catatactac ccaaatatct ggatagcata
     5041 tgctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagcata
     5101 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     5161 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     5221 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     5281 tgctatcctc atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt
     5341 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5401 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5461 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5521 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5581 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5641 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5701 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5761 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5821 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5881 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5941 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6001 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6061 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6121 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6181 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6241 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6301 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6361 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6421 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6481 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6541 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6601 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6661 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6721 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6781 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6841 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6901 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6961 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7021 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7081 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7141 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7201 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7261 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7321 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     7381 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     7441 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     7501 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     7561 gctctagcta gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca
     7621 acgttgttgc cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat
     7681 caatattggc aattagccat attagtcatt ggttatatag cataaatcaa tattggctat
     7741 tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca
     7801 atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     7861 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     7921 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     7981 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     8041 cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac
     8101 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg
     8161 cagtacatct acgtattagt catcgctatt accatg
//
