LOCUS       sequence-234201-        8865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_133270-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3643..3660
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3712..3767
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3766..3785)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6003..6021)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6089..6193
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6194..6262,6263..7054)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6412..6431)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7225..7813
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7714..7733
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7967..7984
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8101..8122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8137..8142,8143..8160,8161..8167)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8175..8191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8180..8202
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8199..8215
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8199..8215
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        8486..8865
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccgaggacc tgaagcccaa gagaaagagc
      841 ggcgacgccc acatcagcat cacccaggac aagaagaaga tcaagagcta catcgagcag
      901 ttcgacgacc ccgagacagt ggaaaagttc ctgcgggacc tgctgaaccc cgacctgatg
      961 ttcccccaga tccccaagga caaagaagat gtgaacgtcg agagcgagag ccaggacgac
     1021 cccctggaca gcaacaccaa ggacgagaac caggaactga ccgacaagag cgagcacgac
     1081 agcaccgccg acaacgtgcc cgccagccag agcatcccca gcatcaacga cgagaaaacc
     1141 gtgaagtcca tccacagccc ccctgacaag cacgaggaca gcgagcctag cgtggcccag
     1201 cacctgtacg acgaggccat gagcatcctg gaaaccgaga gccacaagac cagcgccgac
     1261 aagaccgccc tggaactgct gagcgaggcc agcagacagg gccacaccaa ggcccgggaa
     1321 caggtggcca tgctgaccct gctgggcggc tacaccgccg accctttcca gagcgcctac
     1381 agcgccttcg aggaactgag caacgagggc aaccccagag gccagttcgg cctgggcttt
     1441 ctgtacgcca gcggcctgca cgtgaacgcc agcatccccc acgccctgat ctacctgacc
     1501 ttcagcagcc tgggcggcga caacttcgcc gacatggccc tgggctaccg gtactggaca
     1561 ggcgtgggcg tggaagagga ttgcgaggcc gccctgaccc actaccacag agtggcccag
     1621 gtggtgtaca aagaggtgtc cgagcggagc gagagcggcg gacctacact gctgggccct
     1681 atggtgcgac gggccagact gctggacgag atcgacgcca tgggcaacct ggacggcggc
     1741 gacctgacca tcagcgagga cctgttccag tactaccagt tcatggccta caagaaaaac
     1801 gtgtccgcca tggtcggact gggccagctg tactactacg gccggcacgg cgtggaaatg
     1861 aaccacgaga aggcctttta ctacttcaac ctggccgccg agagcggcag cgccattgcc
     1921 atggcctacc tgggcgagat gtacatggtc ggaagcaccg ccgtgcccgc cgacagcaca
     1981 aaggccctga agtacctgca caagagcgcc gaggaaaaca accccatcgg ccagacaggc
     2041 ctggccctgg cctatctgta tggcagagcc ggcctgcctg tgaagcccgt gatcgccatg
     2101 gaactgtttc tgaaggccgc cgaccagggc tggcctgagg ctcagctgca cctgggcaga
     2161 ctgtttctgg gcacccacgg catcaagacc gactacaagt ccgccctgaa atacttcacc
     2221 atggccagcc agcagggcaa cgtgatcgcc ttctaccacc tggccgagat gcacgccaag
     2281 ggcaccggcg tgctgagaag ctgtagcaca gccgccgagc tgttcaagaa cgtggccgag
     2341 cggggcagat ggtccaagat gttcatgtcc gcctactccg ccttccggaa cagaagatac
     2401 cacgaggcct tcgtgaccta ccagctgctg gccgagctgg gctacgaggt ggcccagagc
     2461 aacgtggcct tcatcctgga agaggaaaag gccaccggca tccctaagga cgagatccac
     2521 aagcgggcct tcacccagtg gcagagaagc gccacccagg gcagcaccag cagcagagtg
     2581 aagctgggcg actactacta ctatggcctg ggcaccgacg tgtcctacca gaaggccatc
     2641 cagcactacc ggatcgcctc cgacctgcac cacaacgccc aggccatgtt caacctgggc
     2701 tacatgcacg agcagggcct gggcctgaag cgggacctgt acctggccaa gcggttctac
     2761 gacatggccg ccgaagccag catcgacgcc agagtggccg tgtatctggc cctgatccgg
     2821 ctgagcttct acttcgtgat ggacttcttc aacgagagca gcttcttcca gtacatcggc
     2881 agcatgttca gcagcagaag ctacgccgac gagcacgagg tggacagcgt gaccgacagc
     2941 accaccaccc ccaccaccag ctctcacccc agcggccacc tgatcagcac aggcgcgccg
     3001 tcgacctcca tcacggccta taagagtgag ggggagtcag cggagttctc cttcccactc
     3061 aaccttggag aggaaagcct gcagggagag ttgagatgga aggcagagaa ggctccttct
     3121 tcccagtcct ggatcacctt ctccctaaag aaccaaaagg tgtctgtgca gaagtctact
     3181 agcaacccca agttccagct gtccgaaacg ctcccactca cccttcagat accccaggtc
     3241 tcccttcagt ttgctggttc tggcaacctg accctgactc tggacagagg gatactgtat
     3301 caggaagtga acctggtggt gatgaaagtg actcagcccg acagcaacac tttgacctgt
     3361 gaggtgatgg gacccacctc acccaagatg agactgatct tgaagcagga gaatcaggag
     3421 gccagggtct ccaggcagga gaaagtgatt caagtgcagg cccctgaagc aggggtgtgg
     3481 caatgtctac tgagtgaagg tgaagaggtc aagatggact ccaagatcca ggttttatcc
     3541 aaagggttga attccggcag cctgcaccac atcctggacg cccagaagat gctgtggaac
     3601 cacagggaca ggaacctgcc tcctctggcc cctctgggcc cccaccacca tcaccaccat
     3661 tgagtctaga cacgtgatta attaaggatc ccccgacctc gacctctggc taataaagga
     3721 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     3781 atgggagggc aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc
     3841 ccgccccgga cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc
     3901 atgtaatccc ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca
     3961 cgggggggga ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat
     4021 gtgcacattt gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac
     4081 tgcaacacaa cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg
     4141 ggacatgtac ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga
     4201 ggggcctgtg tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata
     4261 aggccccctt gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat
     4321 ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt
     4381 agggttagta aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt
     4441 tttatttaca tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag
     4501 tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc
     4561 tccttcgttt agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg
     4621 ctcgaaaaca aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg
     4681 tggcattgtg ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt
     4741 gtaggaatga aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc
     4801 gtaaagactg gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt
     4861 gcaatatgat actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca
     4921 tgttgttaca ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc
     4981 cactggttgt ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata
     5041 aacaaagaca agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc
     5101 ccacacgccg ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt
     5161 aaccccgcta accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg
     5221 gaatacctgc ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag
     5281 gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca
     5341 cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata
     5401 tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
     5461 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
     5521 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     5581 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga
     5641 ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg
     5701 gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     5761 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     5821 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     5881 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     5941 ggtagcatat gctatcctca tgataagctg tcaaacatga gaattttctt gaagacgaaa
     6001 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     6061 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6121 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6181 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6241 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6301 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6361 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6421 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     6481 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6541 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6601 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6661 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6721 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6781 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6841 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6901 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6961 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     7021 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     7081 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7141 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7201 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7261 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7321 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7381 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7441 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7501 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7561 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7621 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7681 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7741 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7801 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7861 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7921 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7981 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     8041 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     8101 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     8161 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     8221 ttacgccaag ctctagctag aggtcgacca attctcatgt ttgacagctt atcatcgcag
     8281 atccgggcaa cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat
     8341 acattgaatc aatattggca attagccata ttagtcattg gttatatagc ataaatcaat
     8401 attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc
     8461 tcatgtccaa tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca
     8521 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     8581 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     8641 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     8701 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac
     8761 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt
     8821 cctacttggc agtacatcta cgtattagtc atcgctatta ccatg
//
