LOCUS       sequence-234204-       11799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 11799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_145420-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..11799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6957..6974
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    7026..7081
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7080..7099)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9317..9335)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9403..9507
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9508..9576,9577..10368)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9726..9745)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10539..11127
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11028..11047
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11281..11298
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11415..11436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11451..11456,11457..11474,11475..11481)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11489..11505
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11494..11516
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11513..11529
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11513..11529
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gcccacaatg
     1201 ccagcatccc tgccaccacc gagagcaaga acctgcgggt gctgtaccgg ttctacaagc
     1261 ccgagaacct ggacttcgtg tacgtggcca gcgtgaacag catccggcag ttcagcgtgg
     1321 acaagctgca cctggtggcc gagcggatca ccggccctca caacttcagc atctactgcc
     1381 tgagcaacgg cgacagctgc gagaagtgca tgcacgtgga cagaagcagc ttccccttcg
     1441 gctacggctc cgacttctgc ctgccccacc ccaccaacaa ctacgtgaag gcctggggca
     1501 ccagcggctt ccctggcccc gagatctaca gcttcaacta cagcgagtgg gaccacgaga
     1561 gccacttcgt gaacgacgac cggatcacct tcgaggacag cctgtacgtg tgcaacaccg
     1621 tgttccacgg ctactgcgag cggctgggcc tgatcaacgt gtcccaggaa agcccctggt
     1681 ccatcggcac ccggaacccc ctggtggaca agaagaacaa gaacccccag gaaatccccg
     1741 tcgtcggcag cctgccctac acccactctg tgctggtcgt gggcagcgag tacatctacg
     1801 tgggcaccga gcccgacggc ctgcaggaac acaccctgat cacctttgac cccctgaccg
     1861 tgcggctgaa gaacttccac ctgtgcagca cccagcccag cttcgagagc agagcccagc
     1921 tgagcaccaa ccccagcctg cggatccagt acaagagcgc cttccggtac agaagcctgc
     1981 ccaacaacct gaacttccag gaccagctgt tcagacccgc ctacgtgtac ttcgtgctgc
     2041 agcagcccga gagcagcagc ttcttccggt ggcagagccg gatcgccaga gtgtgcgacg
     2101 gcgacaagtt catcagcagc tacgtggaac tgaatatcgc ctgcttcgag tgcgtgttcg
     2161 aggacgacga gaagatgttc aacgccctgc acttcagcag ccacggcacc gtgggcaagc
     2221 agctggccag agagctgctg gaaaacttcc agagccagta cgacgacaac ctgtacagca
     2281 agatcgacat caccaccacc gaccacagcg aggtgctgat cgtggccttc gccagccagc
     2341 ccatcgaccc cctgtactac tggtccaccc ggaacgtgtc ccccgacatc gacctgccca
     2401 acgtgcagta caaccccgac agcagctacc ggcacggcag catcctgctg cctggcagcg
     2461 ccatcagcct gttcagcatg acccagatca acgccaagtt cgaccgggtg gtcatgaact
     2521 gcctggacgg catcggcatg cacggcccca gccactttct gcacagcggc agcagcggca
     2581 accccagatg cgtgaaggac gactacagcc gggacctgat ccacgactgc cccagcagcc
     2641 ggcagctgaa ccagttcatc accggcaacg accccgacga cgacatctac agccctgccc
     2701 tgctgtactt caagagcaac gtgaccggca tggccatcac caccgtgtct caggaattca
     2761 ccgtcgccat cttcaccacc gacgacggct acctgtataa gtacgagctg ctgggactgc
     2821 ggaaggtgca cctcctgggc agcctgcagc tggtgcccaa cggccagcct atcaccagcc
     2881 tggccctgga ccacaccggc acaatcgcct ttgccaccag cttccagaag gtgttccgga
     2941 tcgagctgtt caactgcagc gcctacctga cctgccagag ctgcctgaag gcccgggacc
     3001 cctactgcgg ctggtgtatc gccgagggcc ggtgcctgct gttccaccag tgccccctgg
     3061 tcatgagcag agtgaaaacc cccaacgacc tgaagaagaa gcggagcccc aacagattcg
     3121 ccggctccga gtacagcgcc agcgaccggg tgttctggct gcactacaac acctccgtga
     3181 acaagtgccc cgtgatcaga caggtgtccc cacccggcat ccagatcagc accaccccca
     3241 tggtcaccta cgccaaagag aacagccagt actaccagca gaacatcatc ctgagcctgg
     3301 acgccttcct gagccagaac ctgcctaaca gcgacctgag cgaggaaagc ttcagccgga
     3361 accagcccca ccagatcaac agcttcgtgg actccggcaa cccccaccac cctagcctgg
     3421 acagatccag caccgccacc aacagccagc tgttctgcag cttccgcgag gcccccacca
     3481 gcgtgctgca gatggaaagc ctgatcagcg gcaccctgag caacttcgac ctgaacaaga
     3541 tcctgctgaa cgtgaccaga acccccctgc tgacccggac cgtggccaag ctgttctact
     3601 ccagcggcaa gcccatcgag gcccactgcc tgtcccccgc cattgccagc agactgcctc
     3661 agctggaaga tgacaaggcc agcgtgcccc tgctgctgtg gctggaaaaa gtgcggacca
     3721 ccgacgtgga ccccgacaac gtgggagccc tggcccctgc cctgttcgcc gtgtacaact
     3781 gcagccagct gaaggactgc cagtcctgca cccggtccag attcgtgtgc gcctggtgcc
     3841 tgtttgagga ccagtgcgtg cccgtgttcc ccaccctgtc cagcgacggc cagatcacca
     3901 ataccgtgtg caaggacagc agccacctgt actccaagtc ctacgagcag atcaaccccc
     3961 ccttcgacat catcccccct ggccacgccg acgactgccc tagcttcaag ggcagcccca
     4021 agagaatcac cctgaccagc ggctccagcc tgaccgtgaa cctgtatgtg tccaatgtgc
     4081 agaaacagca gatcgtgggc ttcagctgcg ccgagaactg caccaaccag cacgtgcggg
     4141 ccagcttcaa ccccagcaac agcaccgtgt tctgccacat ggaagtgatg aacctgaccg
     4201 agcacctgag cacccggctg ctgaacaaca acgacatcca gcggagccac gacaccgccg
     4261 tgatctgcga cctggacctg tactggcacg gcctgaacag ccacgacgcc aagggccacc
     4321 ggatggtcaa cgtggaccag gtgcaggtcg aggtgtacgc ctgcgagtgg ctggccgagt
     4381 actgcgacaa gtgcctggac ctgcccccca gattcggctg ctcttggtgc gtgtccgccc
     4441 aggaaaacat ccacatcaac gactacgaga acaagaatat cctgaacgag ggcaagtgcc
     4501 ggacccagaa cagctgcgtg acccggaagt acttcaacgg ctctatcgcc gtgcggcagc
     4561 acagcatcag acccggcgac gtgtgccccg accccgagat cctgaccctg agccccacca
     4621 atgccaccct gaccggccag ccaatcctga ccatcaccgg ccggaacctg ggcagaagcg
     4681 cctccgatat cgtcgaggtg ttcctggacc tccagagcaa gatccagtgc atcgtgctga
     4741 gcgagacata ccagagaagc cacaagttca tgtgcaagct ggtggccgct ccccacctga
     4801 gcctgcctgt gtccggcaag ctgaaggtgg tcatcagcaa caagagatac gaggccttca
     4861 gccctgtgtt ccacttcgcc gtgccccatc tgatccacgc caccccccag agaggcccta
     4921 aggctggcgg caccagactg tacctgagcg gcaccaacct gaacatcggc cggcagcggg
     4981 ccgtgtacct gtacctgcct accatctccg gcaccagcga cgagagctac caggaccggt
     5041 tcagcgagag cgacgtgcag gtcaaatgcg agatcgagca ggaaagcgag acactgatcg
     5101 tgtgcctgac cacccctctg ctgaaagaaa ccatgaacac catgaagaac aaaaagcagt
     5161 tcaagcggca cagcctgttc gtgaacagct actacaacga caacaacaag accctcatca
     5221 cccccgagac aaccacaatc gccgaggacg agcaccacat ccccctgagc ctcgtgatca
     5281 tgcacgacat gaccctgacc tacctgagcc acagcttcag cttcatctac acccccaacc
     5341 cctccatcct ggaagtgcag cggcagaacg tgctcgtggg cggaggcacc acagtccggg
     5401 tgctgggcag cttcctgttc gtggtgcagg acccccggct ggtgttctac ttctccggaa
     5461 gcgagtacag catgagctgc ctggtgtcct ccaatggcca gctggactgt ctgagcccca
     5521 gcctgggcat gttccaggac acccccttcg tgaaggacat gaacgacaag cacatctaca
     5581 ccagcgagaa gctggacaac ctgatgcaga gcagcaccag cgtgtggacc aacggcaaag
     5641 aaagcgtgac cagctgcctg cccatcagcc agcccctggt gctgtcccag ctgaagtcca
     5701 acagagagtt ctacagcagc gccttcacca gccacccccc cgactggccc acagacgtgc
     5761 cctacggctt catcatggac ggcgtgcacc agctgagaat cttcggactg attaaggtgt
     5821 accaggaccc catcgtgatc cctttccccg acggcatccg ggtggaaaac ctggacgacg
     5881 attacctgac cgacgatctg atgaacgacc agcatgtcga gctgaacacc taccagcggc
     5941 agatcagcaa ccagagcctg gaacccagca gccagagcgt gtccagcacc aactcccaca
     6001 cactgagcaa ccacatcgac tacagcaatg tgcggcagct gatccggatc cacggccact
     6061 tcgacgccct gatcaatgcc cccgagctgg ccaagtccga tgagctgatc gtgcggatcg
     6121 gcgacagcct gatctgcgaa gtgaactcta tcatcctcac cgagatcaga tgcgatctgg
     6181 accggaagat gatcctgaag caccaggact accccgtcga ggtgcagttc ggccggttcc
     6241 tgatctacag acccggcagc gtcagattcg tggccctgag acaggccgcc ctgcggagcc
     6301 agattggcgc gccgtcgacc tccatcacgg cctataagag tgagggggag tcagcggagt
     6361 tctccttccc actcaacctt ggagaggaaa gcctgcaggg agagttgaga tggaaggcag
     6421 agaaggctcc ttcttcccag tcctggatca ccttctccct aaagaaccaa aaggtgtctg
     6481 tgcagaagtc tactagcaac cccaagttcc agctgtccga aacgctccca ctcacccttc
     6541 agatacccca ggtctccctt cagtttgctg gttctggcaa cctgaccctg actctggaca
     6601 gagggatact gtatcaggaa gtgaacctgg tggtgatgaa agtgactcag cccgacagca
     6661 acactttgac ctgtgaggtg atgggaccca cctcacccaa gatgagactg atcttgaagc
     6721 aggagaatca ggaggccagg gtctccaggc aggagaaagt gattcaagtg caggcccctg
     6781 aagcaggggt gtggcaatgt ctactgagtg aaggtgaaga ggtcaagatg gactccaaga
     6841 tccaggtttt atccaaaggg ttgaattccg gcagcctgca ccacatcctg gacgcccaga
     6901 agatgctgtg gaaccacagg gacaggaacc tgcctcctct ggcccctctg ggcccccacc
     6961 accatcacca ccattgagtc tagacacgtg attaattaag gatcccccga cctcgacctc
     7021 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     7081 actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc
     7141 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     7201 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     7261 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     7321 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     7381 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     7441 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     7501 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     7561 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     7621 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     7681 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     7741 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     7801 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     7861 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     7921 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     7981 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     8041 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     8101 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     8161 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     8221 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg
     8281 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc
     8341 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg
     8401 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata
     8461 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact
     8521 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt
     8581 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     8641 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     8701 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     8761 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     8821 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     8881 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     8941 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     9001 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     9061 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     9121 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     9181 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     9241 ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt
     9301 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     9361 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     9421 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     9481 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     9541 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     9601 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     9661 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     9721 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     9781 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     9841 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     9901 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     9961 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
    10021 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
    10081 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
    10141 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
    10201 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
    10261 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
    10321 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
    10381 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    10441 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    10501 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    10561 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    10621 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    10681 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    10741 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    10801 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    10861 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    10921 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    10981 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    11041 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    11101 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    11161 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    11221 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    11281 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
    11341 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
    11401 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
    11461 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    11521 agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca
    11581 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
    11641 tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
    11701 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
    11761 acatttatat tggctcatgt ccaatatgac cgccatgtt
//
