LOCUS       sequence-234205-        8292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_149740-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3070..3087
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3139..3194
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3193..3212)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5430..5448)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5516..5620
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5621..5689,5690..6481)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5839..5858)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6652..7240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7141..7160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7394..7411
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7528..7549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7564..7569,7570..7587,7588..7594)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7602..7618
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7607..7629
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7626..7642
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7626..7642
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7913..8292
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gcctttgagt gcggcaagcc cgccatctac
      841 ctgaacaacg agttctgggt caccgacctg acccagggct gcctggaaga tgagctgagc
      901 atcctgaact actgcaagaa ggtgtacagc gagcggaaca tcaccgccgt ggtgtccgcc
      961 cctcccatcg acgtggtgct gagcgattgg tgcgagttcg cccacaagaa actggccaag
     1021 tgccagagca tcggcgacaa gcggcacgag atcaagccct tcatctgcct ggacaacatc
     1081 cccatgacca ccctgttcac ccccgtgggc tgccagctgt cctccctgga caacgagcgg
     1141 agctacgtgt gcgagaacta caagttctgg gagagcagca ccagagaggc ctgcctgatc
     1201 cggaacatgc ggttccagag ctacctgccc tacaagccct gcatcagcga gaaggacttc
     1261 cggtccatgt acttcgccgc tgccaaggtc gtgtgctgca agaacctgga cagcgacttc
     1321 accaactctg ccgccgtgat gaagcccctg accgaccccg gcaacaagac cagcagcatg
     1381 agccacaagc ccagcagact gggcaacaag ctgaccacca ccgagaacgg catctgggac
     1441 agcgacaaga ccgtgatcaa ctacctgagc ttcaccaagc agaacagcag cagcggcctg
     1501 ctgatccccg agagagagcg gtacagccag gccaagctgg ccctgagcga cgacctgcac
     1561 aagagagaga aggccctgga acggaagttc agcagcgagg aaagcctgat cagccccgag
     1621 gactggctga acgagcccgt gaagtcccag ctggaagagg acagcctggt gcaggaattt
     1681 ctgagcaagt tcgtgaagct ggaaaaccag agcgagcagg acagaaagac cctggaaacc
     1741 atccaccacc agcggatcga ggcccagatg ctggaacggc ggaacgccat gcagaacgcc
     1801 tgggagaagg ccgtgaacat cgagaacccc aaccaccccg agctgagccc ccctctgctg
     1861 gcctacgcca actacctgcg gaacaacaag taccgggacc tggaaagcaa gagcaaggcc
     1921 gtgctggtgg ccgacgtgac cctgcccgac atgatcaagc tgcccacctt ccaggaacag
     1981 gtcagcctga aggccgagga aatcatcacc aagcaccggc accacctgga acccctgtac
     2041 agcgataagc tgactaccaa caagagcaac aagtcccgga tcaagccccc cagacggcac
     2101 aagatgctga tcaaccaccc cttcggcgga aacaagaaca agaacttcag caacagccac
     2161 cagatgaaga ccaccaccat catccccacc gtgtccaacc tggtggatat cgacgtgaag
     2221 tccttcctga gcaccgtgga ctacagcagc tccctgagcc tgaaccagaa ccagcacacc
     2281 ccccacatca acagcagcga gatgaccgag aacaagacca ccgtgcagtt cggcagctac
     2341 cagaacatca gcgtggtcat gctgcactcc gacaaggtgc tgaacaccag caacagcagc
     2401 accgtgaacc tgagcacagg cgcgccgtcg acctccatca cggcctataa gagtgagggg
     2461 gagtcagcgg agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg
     2521 agatggaagg cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac
     2581 caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc
     2641 ccactcaccc ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc
     2701 ctgactctgg acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact
     2761 cagcccgaca gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga
     2821 ctgatcttga agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa
     2881 gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag
     2941 atggactcca agatccaggt tttatccaaa gggttgaatt ccggcagcct gcaccacatc
     3001 ctggacgccc agaagatgct gtggaaccac agggacagga acctgcctcc tctggcccct
     3061 ctgggccccc accaccatca ccaccattga gtctagacac gtgattaatt aaggatcccc
     3121 cgacctcgac ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     3181 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc
     3241 ggagatctct agctagagga tcgatccccg ccccggacga actaaacctg actacgacat
     3301 ctctgcccct tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc
     3361 caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga
     3421 ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc
     3481 ctggagcaga ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt
     3541 ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta
     3601 cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc
     3661 aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata
     3721 tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag catatgttac
     3781 ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat
     3841 atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt
     3901 agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct
     3961 cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt
     4021 tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga
     4081 ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca
     4141 caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa
     4201 tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta
     4261 tggctatggg caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc
     4321 caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag
     4381 agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa
     4441 cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa
     4501 ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa
     4561 taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc
     4621 ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga
     4681 taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa
     4741 gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg
     4801 ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
     4861 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     4921 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     4981 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
     5041 gggtagcata tgctatccta atagagatta gggtagtata tgctatccta atttatatct
     5101 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     5161 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     5221 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     5281 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     5341 agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatga taagctgtca
     5401 aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5461 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5521 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5581 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5641 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5701 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5761 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5821 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     5881 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5941 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6001 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6061 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6121 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     6181 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6241 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6301 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6361 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6421 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6481 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6541 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6601 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6661 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6721 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6781 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6841 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6901 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6961 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7021 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7081 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7141 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7201 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7261 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7321 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7381 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     7441 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     7501 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     7561 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     7621 tcacacagga aacagctatg accatgatta cgccaagctc tagctagagg tcgaccaatt
     7681 ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg
     7741 cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta
     7801 gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
     7861 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga
     7921 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     7981 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     8041 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     8101 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     8161 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     8221 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
     8281 gctattacca tg
//
