LOCUS       sequence-234206-        9903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9903)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 9903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_155810-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..9903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4681..4698
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4750..4805
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4804..4823)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7041..7059)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7127..7231
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7232..7300,7301..8092)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7450..7469)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8263..8851
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8752..8771
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9005..9022
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9139..9160
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9175..9180,9181..9198,9199..9205)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9213..9229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9218..9240
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9237..9253
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9237..9253
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        9524..9903
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccctgatcc agaacatcaa cagccccatc
      841 gaccacgagt actacatcga ggaagagatc cccatcggca ccatcatcgg caatatcatg
      901 gacgacctga tcatgaagta catcgacttc ggcctgacca agttcctgca caagctgaac
      961 tcccccatca acgacaacga ccacagctac agcctgatga tcatgaactt cgccgacccc
     1021 tacgtgaacc tgttcgacat caagctggac ggccggatca tcctgagcag acggctggac
     1081 cgggaccaga tctgccccaa gcagcagcac accgacctgc ccctggtcaa catcgacagc
     1141 gcccagttca gcagcgtgta caccagcacc caccggcaga acatcctgca gaccgagaag
     1201 atcagctgca gcatcattct gcagatcggc ctgttcatcc accagaagaa cggcatcatc
     1261 aacaaagtga acaccgaggc caacaacaag aagcagatcc ggctgttcca catccggatc
     1321 aacatccagg acatcaatga caacgccccc tactggcagg gccatctgca gcggttccgg
     1381 atcaccttcc tggacggcga ccccgtgggc agcagaagaa acatcccccc tgccatggac
     1441 aacgacgagg gcatcaacgg cctgatcacc tacgagctga gccccgtgaa cagcttcgac
     1501 aagagccaca tccccttcag cctgctggaa gatagcatcg acggcctgca cctgtacgcc
     1561 accaagcaga tcgacagaga ggaacagagc cagtacaagc tggtgctgaa ggccaccgac
     1621 ggcagctact ccctgagcag ctctaccagc agcgccctga gcaacgagag caccttcaag
     1681 cggttcacct ccaccctgta cgtggacatc ttcatcgagg acgtgaacga caacaccccc
     1741 cagttcaccc agcccatctt caccaccagc atgcctatcg ccgagacaac ccccgtgggc
     1801 gccaccgtgc tgctgctgaa cgccacagac ctggacagcg gcatcaatgg catcttccac
     1861 ttcagcttca gcaaggacca ctactggcag cccgccgaga agattgcccg gcactacttc
     1921 gacatccggc ccaacggcca gatcattgtg aagcagcccc tgaatgtgga caagctggac
     1981 cagatcaata gccacatcaa ccacaacaac aacaaaatcg tgctgcccaa catcagaggc
     2041 gcctaccagc tgaaggacga catcgacgag aatatcaaca agggcgtgaa cgtgcagttc
     2101 cggttccggg ccgtggtgga agatgaggcc acccggccct acaccagatc cagcgaggcc
     2161 accgtgatca tcctggtcac cgacgagaac gacgagtccc ccgtgatctc cctgctgccc
     2221 aatcccgagc tgatcagcag caacgccctg gacgccgtga ccaccagcaa cagcttcagc
     2281 tacttctggg tgttcgagaa ccagcccgtg ggcaccacag tggccaccgt gcaggcctac
     2341 gaccccgact ttggcggcca ggaccaggtg gaatgcaacc tgaaggacag caacttcagc
     2401 ctgatccggc tgaccagcca gacccagccc gagttcgacc ggaacaccga agtgaacctg
     2461 ctgaccgagt accacctgat caccgcccgg atcctggacc gcgagagcac cagccaccag
     2521 gtggtcacca tcgtgtgcag cgacatggtc ggacactaca ccgagcggaa caccaccgtg
     2581 cgcatcctgg acgagaatga caaccggccc aagttcagcc acgagatcct gtatctgcag
     2641 gtcgacgaaa acgcccctta cggcacccag gtcatccagc agagccagaa agtgaaccgg
     2701 ggccacttca acaacgtgca ccagaccggc agcctgcagg gcctgctggc cagcgacctg
     2761 gatatcggaa tcaacagcca gatgacattc ttcctgttcg aggacggcat caacagcacc
     2821 aagttcaaga tcgacagcca cacaggcatc atcaccacca tggatatctt cgaccgggaa
     2881 gtgaaagaga agtacaccct gatcgccctg gccgtggacc acggcaatcc tccactgacc
     2941 ggcaccgcca cagtgcagat catcatcaac gacctgaacg acaatggccc cgtgttcagc
     3001 accaacaact acacattcca tctgcaggaa aaccaggccc ggtccaccat cgtgggccag
     3061 gtcaacgcca ccgacgtgga ctctagcgcc tacggccccc tggaatactc cttcgccaac
     3121 gaccccgata gcgtgaactt caccatcgac aagatcaccg gcatcatccg gaaccggcag
     3181 agcctggaca gagaagagaa gtcccagtac atcttccggg tgctggtcaa ggacaccaag
     3241 atccggccca gcgagttcaa cagcatcagc atcaacaagc tcaacagcaa gagcatccag
     3301 tacaccggca ccagcaccgt gaccgtgatc gtggacgatg tgaacgataa ctggcctgtg
     3361 ttcgtgtccc ccaacagcac cgccaacacc ctggccattg ccctggacca gaagatcaca
     3421 ggctacaagc tggcctacat ccaggccgag gacaaggatg agggcaacaa tggcaccatc
     3481 acctacaaca tcctgaccgg caacaactac ggcctgttcg gcctggacag caccagcggc
     3541 ctgctgtacc tgagcaaccc cttcagcgac aacaacaagc tgagcgacca caacaatgag
     3601 ctgtacgaca gcagcaacac cagcaacctg cccaccaacg acgagttcaa tggcctgctg
     3661 cacaagagca gcatccacat cctgacactg gaagcctgcg accagggcca ggaccccaag
     3721 cccagatgca ccctgtacaa caacctgaag atcatgattc agaacaacaa agagaaggac
     3781 gtccacaccc tggaaagcaa gcagttccag tacaactccc ccaaccagtt catcaacgcc
     3841 cctggcctga ccagcgccga gacagtgctg agcagcgcct tcaagcagaa ccaccccaac
     3901 cacatccaga acaacttcaa ctccatccag gaattcaaca acgacaacct gtaccagaag
     3961 gccacaggcc tgacctacgg caacctgctg atgagcaagc agaatcacaa gctgtccagc
     4021 tacaccacag gcgcgccgtc gacctccatc acggcctata agagtgaggg ggagtcagcg
     4081 gagttctcct tcccactcaa ccttggagag gaaagcctgc agggagagtt gagatggaag
     4141 gcagagaagg ctccttcttc ccagtcctgg atcaccttct ccctaaagaa ccaaaaggtg
     4201 tctgtgcaga agtctactag caaccccaag ttccagctgt ccgaaacgct cccactcacc
     4261 cttcagatac cccaggtctc ccttcagttt gctggttctg gcaacctgac cctgactctg
     4321 gacagaggga tactgtatca ggaagtgaac ctggtggtga tgaaagtgac tcagcccgac
     4381 agcaacactt tgacctgtga ggtgatggga cccacctcac ccaagatgag actgatcttg
     4441 aagcaggaga atcaggaggc cagggtctcc aggcaggaga aagtgattca agtgcaggcc
     4501 cctgaagcag gggtgtggca atgtctactg agtgaaggtg aagaggtcaa gatggactcc
     4561 aagatccagg ttttatccaa agggttgaat tccggcagcc tgcaccacat cctggacgcc
     4621 cagaagatgc tgtggaacca cagggacagg aacctgcctc ctctggcccc tctgggcccc
     4681 caccaccatc accaccattg agtctagaca cgtgattaat taaggatccc ccgacctcga
     4741 cctctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     4801 tctcactcgg aaggacatat gggagggcaa atcatttggt cgagatccct cggagatctc
     4861 tagctagagg atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc
     4921 ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc
     4981 cctgttccac atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg
     5041 acatccttat aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag
     5101 actttgcagt ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc
     5161 tcttacacca atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct
     5221 acaccaacgt caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca
     5281 ttagcaatag tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg
     5341 ggtagtagta tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga
     5401 agcatatgct atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac
     5461 cccatgagct gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca
     5521 ttttagtcac aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct
     5581 tcgcctgctt cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag
     5641 taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt
     5701 ggacgggggg ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct
     5761 tgggcaataa atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc
     5821 atggggtggg gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg
     5881 gcaacacata atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg
     5941 accaagacag gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac
     6001 gccgacagca gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc
     6061 acgccaatgg ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt
     6121 gggggcacgc gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt
     6181 aataacttgg ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac
     6241 cactaatggc accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc
     6301 gattgctgcg atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt
     6361 tgttggtcct catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta
     6421 gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat
     6481 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     6541 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     6601 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     6661 atgctatcct aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat
     6721 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc
     6781 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     6841 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     6901 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     6961 tatcctaatc tgtatccggg tagcatatgc tatcctcatg ataagctgtc aaacatgaga
     7021 attttcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     7081 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7141 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7201 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7261 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7321 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     7381 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     7441 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     7501 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     7561 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     7621 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     7681 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     7741 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     7801 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     7861 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     7921 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     7981 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8041 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8101 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8161 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8221 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8281 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     8341 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8401 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8461 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8521 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8581 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8641 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8701 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8761 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8821 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     8881 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     8941 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9001 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9061 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     9121 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     9181 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     9241 aaacagctat gaccatgatt acgccaagct ctagctagag gtcgaccaat tctcatgttt
     9301 gacagcttat catcgcagat ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg
     9361 taggtatgga agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt
     9421 tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat
     9481 atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg attattgact
     9541 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     9601 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     9661 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     9721 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     9781 agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     9841 atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     9901 atg
//
