LOCUS       sequence-234222-       12105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral gene expression vector for doxycycline inducible PGBD5 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Henssen AG, Koche R, Zhuang J, Jiang E, Reed C, Eisenberg A, Still 
            E, MacArthur IC, Rodriguez-Fos E, Gonzalez S, Puiggros M, Blackford 
            AN, Mason CE, de Stanchina E, Gonen M, Emde AK, Shah M, Arora K, 
            Reeves C, Socci ND, Perlman E, Antonescu CR, Roberts CWM, Steen H, 
            Mullen E, Jackson SP, Torrents D, Weng Z, Armstrong SA, Kentsis A
  TITLE     PGBD5 promotes site-specific oncogenic mutations in human tumors.
  JOURNAL   Nat Genet. 2017 May 15. doi: 10.1038/ng.3866.
  PUBMED    28504702
REFERENCE   2  (bases 1 to 12105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2017 May 15. doi: 10.1038/ng.3866."
COMMENT     Sequence Label: pINDUCER21-PGBD5
FEATURES             Location/Qualifiers
     source          1..12105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4334..4358)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4411..4437
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4657..5835
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4888..5826
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5783..5803
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5905..5938
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(5945..6562,6563..6568,6569..6604,6605..6607,6608..6643,
                     6644..6646,6647..6682,6683..6691)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6744..7317
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6911..6928)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7138..7157
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7333..7335,7336..7338,7339..8052)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7333..7335,7336..7338,7339..8049)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7378..7399)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7639..7658)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7986..8007
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8188..8207
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8495..9083
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8548..8568)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8966..8977)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9158..9391
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9510..9528)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9596..9700
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9701..9769,9770..10561)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9919..9938)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10732..11320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11221..11240
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11474..11491
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11731..11752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11767..11772,11773..11790,11791..11797)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11805..11821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11810..11832
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11829..11845
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11829..11845
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11895..11914
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggcttaagg agtttaaaca ccatggccga gggcggcggg
     2761 ggcgcgcgga ggagggcgcc ggcgctgctc gaggctgccc gcgcgcgcta cgagagcctg
     2821 cacatctcgg acgacgtgtt cggcgagtcc ggcccggaca gcggcgggaa ccccttctac
     2881 agcacctcgg ccgcctcgcg ctcctcctcg gccgcctcct cggacgacga gcgcgagccc
     2941 ccgggacccc caggggccgc cccgccaccg ccccgcgccc cggacgcaca ggagccggag
     3001 gaggacgagg ccggcgcggg ctggagcgca gcgctgcggg accgcccgcc cccgcgcttc
     3061 gaggataccg gcggtcccac ccgaaagatg ccccccagcg ccagtgccgt ggacttcttc
     3121 cagctctttg tcccagacaa cgtcctcaag aacatggtgg tgcagacaaa catgtatgcc
     3181 aagaagttcc aggagcggtt tgggagcgac ggagcctggg tggaggtgac gctgacggag
     3241 atgaaggcgt tcctgggcta catgatctcc accagcatct cccactgcga gtccgtcctc
     3301 agcatctgga gcggaggctt ctacagcaac cgcagcctcg ccctcgtcat gagccaggcc
     3361 cgcttcgaga agatcctcaa gtacttccac gtcgtggcct tccgctccag ccagaccacg
     3421 cacgggctct acaaggtcca gcccttcctc gactccctgc agaacagctt cgactctgcc
     3481 ttcaggcctt cccaaaccca ggtgctacat gaacccctga tcgatgagga tcctgtattc
     3541 attgccacgt gcacagagcg ggagctgcga aagaggaaaa agcggaaatt cagcctctgg
     3601 gtcagacaat gttcttccac tggcttcatc atccagattt atgtccacct gaaggaaggt
     3661 gggggcccag atggcctgga tgcgctgaag aataagcccc agctccacag catggtggcc
     3721 aggagcctgt gccggaacgc ggcaggcaag aactacatca ttttcacggg gcccagcatc
     3781 accagcctga cgctgtttga agagtttgag aagcaaggga tttactgctg cggcttgctc
     3841 cgcgcgcgga agagtgactg caccggcctc ccactgtcca tgctgaccaa cccagccaca
     3901 cccccggccc ggggccagta ccaaatcaag atgaagggga acatgtcctt gatctgctgg
     3961 tacaacaaag gacacttccg cttcctgacc aacgcctact ccccggtgca gcagggagtc
     4021 atcatcaaaa ggaagagtgg ggagatccca tgccccttgg ccgtggaggc gtttgccgct
     4081 cacctgagct acatctgcag atacgatgac aaatacagca agtatttcat ttctcataaa
     4141 ccaaacaaga cctggcagca ggtgttctgg ttcgccatca gcatcgccat caacaatgcc
     4201 tacatcctgt acaaaatgtc agacgcctac cacgtgaaga ggtacagccg ggcgcagttt
     4261 ggagagagac tcgtcagaga gctgctgggc ttggaggatg cctctccgac ccactagctc
     4321 gagtgcggcc gcaacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc
     4381 cgctcgagtc tagagggccc gcggttcgaa tacccatacg acgtcccaga ctacgcttag
     4441 taatgattaa ttaaactaga aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     4501 tggagtaacc cacccaagat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc
     4561 cccctcctca cggcgagccg cgtaagatct ggcctccgcg ccgggttttg gcgcctcccg
     4621 cgggcgcccc cctcctcacg gcgagcctag tcgtgaggct ccggtgcccg tcagtgggca
     4681 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
     4741 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     4801 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     4861 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
     4921 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
     4981 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     5041 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     5101 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     5161 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     5221 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     5281 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     5341 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     5401 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     5461 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     5521 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     5581 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     5641 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     5701 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     5761 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     5821 tttcaggtgt cgtgaagcgg ccgccatgct agtaaattgt ccgctaaatt ctggccgttt
     5881 ttggcttttt tgttagacgg atccataact tcgtataatg tatgctatac gaagttatgc
     5941 caccatgtct agactggaca agagcaaagt cataaacgga gctctggaat tactcaatgg
     6001 tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     6061 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     6121 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
     6181 gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt
     6241 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     6301 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     6361 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     6421 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg
     6481 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     6541 acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat
     6601 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     6661 cgattttgac cttgacatgc tccccgggta actaagtaag gatccgcggc cgcactagag
     6721 gaattccgcc cctctccccc ccccccctct ccctcccccc cccctaacgt tactggccga
     6781 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     6841 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     6901 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     6961 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     7021 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     7081 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     7141 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     7201 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     7261 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
     7321 taccggtcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     7381 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     7441 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     7501 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     7561 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     7621 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     7681 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     7741 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     7801 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     7861 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     7921 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     7981 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     8041 ctgtacaagt aagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     8101 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     8161 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     8221 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     8281 gcatcgcctt ctatcgcctt cttgacgagt tcttctgatg tacaagtaaa gcggccgcga
     8341 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     8401 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt ttagtccctc
     8461 ccaattcgat atcaagctta tcgcgataga tcctaatcaa cctctggatt acaaaatttg
     8521 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     8581 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     8641 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     8701 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     8761 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     8821 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     8881 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     8941 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     9001 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     9061 ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct
     9121 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     9181 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     9241 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     9301 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     9361 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     9421 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa
     9481 agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     9541 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     9601 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     9661 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     9721 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     9781 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     9841 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     9901 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     9961 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    10021 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    10081 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    10141 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    10201 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    10261 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    10321 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    10381 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    10441 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    10501 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    10561 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    10621 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    10681 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    10741 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    10801 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    10861 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    10921 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    10981 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    11041 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    11101 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    11161 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    11221 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    11281 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    11341 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    11401 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    11461 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    11521 gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt
    11581 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    11641 ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga
    11701 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    11761 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    11821 atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc
    11881 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    11941 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    12001 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    12061 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
