LOCUS       sequence-234225-       14680 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14680)
  AUTHORS   Nam HS, Benezra R
  TITLE     High levels of Id1 expression define B1 type adult neural stem 
            cells.
  JOURNAL   Cell Stem Cell. 2009 Nov 6. 5(5):515-26.
  PUBMED    19896442
REFERENCE   2  (bases 1 to 14680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2009 Nov 6. 5(5):515-26."
COMMENT     Sequence Label: pB6-ROSA26::lacZ
FEATURES             Location/Qualifiers
     source          1..14680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     596..613
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     597..613
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1986..2002
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2016..2049
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(2114..2179,2180..2217)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     CDS             2268..5309
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQ"
     CDS             5334..6137
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5397..5416)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6007..6026
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6175..6399
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6472..6505
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6558..7368
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     misc_feature    6882..7531
                     /label=HA-R
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        10889..11388
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10924..10947)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11321..11340
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             11415..11987
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          12064..12129
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    12203..12427
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(12489..12509)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12489..12507)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12528..12544)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12528..12544)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12541..12563)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12552..12568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12576..12582,12583..12600,12601..12606))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12621..12642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12759..12776)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12930..13518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13010..13029)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13689..14480,14481..14549))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14312..14331
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14550..14654)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttccat tcgccattca ggctgcgcaa
      481 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
      541 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      601 aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg agctccaccg
      661 cggtggcggc cgcggcaggc cctccgagcg tggtattaag tgccggagcg cgaaatttac
      721 tgcaacacta cagttttccg ggaaacgtgc gcgaactgga acatgctatt catcgggcgg
      781 tagttctggc gagagccacc cgcagcggcg atgaagtgat tcttgaggcg caacattttg
      841 cttttcctga ggtgacgttg ccgacgccag aagtggcggc ggtgcccgtt gttaagcaaa
      901 acctgcgtga agcgacagaa gcgttccagc gtgaaactat tcgtcaggca ctggcacaaa
      961 atcatcacaa ctgggctgcc tgcgcgcgga tgctggaaac cgacgtcgcc aacctgcatc
     1021 ggctggcgaa acgtctggga ttgaaggatt aaataatccc ggcctgatag aaatcctgca
     1081 ggtttattgc gccggtgagt ttgccgtttt catccaccac cggtgcggca gtgattttgc
     1141 gcttcatcag gatctctttg gcgtcgatgg cgcgactttg cgattgcaac gtggtgccgc
     1201 cgaccgtcat cgcttcattg actggcgtgg tgagtgcgcc gccgccaacc agccagcgac
     1261 gtaaatcgcc gtcggtaaag acgccttgta cctgttgttg agcgtcacat accgccacca
     1321 gccccagacc ggtgcggctg agttccagca tcgcatccat cacgctggcg gttaacgcca
     1381 cctgtgggat ggcatcgtca cggcgcatca gatgatgcac tttattcagc aagcgagcgc
     1441 ccagtgcccc ggctgggtgg gagcgggcaa aatcttcttc attaaatccg cgcgcctgca
     1501 tgaccgccat cgccagcgcg tcacccatca tcagggtatt gacggtgctg gaggtcggcg
     1561 caaggtgcat cgggcaggct tcgcgttcta cggagatatc cagcaccgct tttgccgcca
     1621 ggcccagcgg tgacgtcggt ttgccggtca tcgccagcag cgcgatagat ttatcttcca
     1681 gacgcggaat aatcagatcc agttccttcg cgccaccgga gtaagagata aacagcatca
     1741 catcgcggct ttcgatcatc cccagatcgc cgtgcagcgc ttctgccgga tggacaaaaa
     1801 aagccggagt gccggtactg gcaagcattg cggcgatttt cttaccaatg tggcccgatt
     1861 tgccaattcc cgaaaccacc actttgcctt cacagtgcag gatgatattg gcggcgcgaa
     1921 caaaatcatc gcccagacgt tccggtaaac ggcttgcttc ctgcaactcc agtctttgcg
     1981 gccgctctag aactagtgga tccggaaccc ttaatataac ttcgtataat gtatgctata
     2041 cgaagttatt aggtccctcg acctgcagga atttcgaggc tagaactagt ggatcccccg
     2101 ggctgcagat ctgtagggcg cagtagtcca gggtttcctt gatgatgtca tacttatcct
     2161 gtcccttttt tttccacagc tcgcggttga ggacaaactc ttcgcggtct ttccagtggg
     2221 gatcgacggt atcgataagc ttgatgatct gtgacatggc ggatcccgtc gttttacaac
     2281 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     2341 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     2401 gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct
     2461 ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc
     2521 acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc aatccgccgt
     2581 ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct
     2641 ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt
     2701 ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc
     2761 tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg
     2821 acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct
     2881 cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg
     2941 atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact
     3001 acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc
     3061 ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc
     3121 tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg
     3181 ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc
     3241 gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag
     3301 gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg
     3361 tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc
     3421 cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag
     3481 ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc
     3541 taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg
     3601 tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac gcgctgtatc
     3661 gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca
     3721 ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc
     3781 cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc
     3841 tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact
     3901 ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac tgggtggatc
     3961 agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg
     4021 gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc
     4081 cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg
     4141 ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc
     4201 actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg
     4261 ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc
     4321 aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc
     4381 acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg
     4441 cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta
     4501 ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata
     4561 aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca
     4621 ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg
     4681 cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg
     4741 cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc
     4801 ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtgg
     4861 cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag
     4921 agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg
     4981 cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga
     5041 gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg
     5101 gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc
     5161 gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga
     5221 ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg agcgccggtc
     5281 gctaccatta ccagttggtc tggtgtcagg ggatcccccg ggctgcagcc aatatgggat
     5341 cggccattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     5401 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     5461 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     5521 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     5581 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     5641 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     5701 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     5761 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     5821 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     5881 acggcgatga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     5941 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     6001 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     6061 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     6121 ttgacgagtt cttctgaggg gatcaattct ctagagctcg ctgatcagcc tcgactgtgc
     6181 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     6241 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     6301 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     6361 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     6421 gctggggctc gatcctctag agtcgaatca agctgatccg gaacccttaa tataacttcg
     6481 tataatgtat gctatacgaa gttattaggt ccctcgacct gcagcccaag ctagcttatc
     6541 gataccgtcg acctagaaga tgggcgggag tcttctgggc aggcttaaag gctaacctgg
     6601 tgtgtgggcg ttgtcctgca ggggaattga acaggtgtaa aattggaggg acaagacttc
     6661 ccacagattt tcggttttgt cgggaagttt tttaataggg gcaaataagg aaaatgggag
     6721 gataggtagt catctggggt tttatgcagc aaaactacag gttattattg cttgtgatcc
     6781 gcctcggagt attttccatc gaggtagatt aaagacatgc tcacccgagt tttatactct
     6841 cctgcttgag atccttacta cagtatgaaa ttacagtgtc gcgagttaga ctatgtaagc
     6901 agaattttaa tcatttttaa agagcccagt acttcatatc catttctccc gctccttctg
     6961 cagccttatc aaaaggtatt ttagaacact cattttagcc ccattttcat ttattatact
     7021 ggcttatcca acccctagac agagcattgg cattttccct ttcctgatct tagaagtctg
     7081 atgactcatg aaaccagaca gattagttac atacaccaca aatcgaggct gtagctgggg
     7141 cctcaacact gcagttcttt tataactcct tagtacactt tttgttgatc ctttgccttg
     7201 atccttaatt ttcagtgtct atcacctctc ccgtcaggtg gtgttccaca tttgggccta
     7261 ttctcagtcc agggagtttt acaacaatag atgtattgag aatccaacct aaagcttaac
     7321 tttccactcc catgaatgcc tctctccttt ttctccattt ataaactgag ctattaacca
     7381 ttaatggttt ccaggtggat gtctcctccc ccaatattac ctgatgtatc ttacatattg
     7441 ccaggctgat attttaagac attaaaaggt atatttcatt attgagccac atggtattga
     7501 ttactgctta ctaaaatttt gtcattgtac acatctgtaa aaggtggttc cttttggaat
     7561 gcaaagttca ggtgtttgtt gtctttcctg acctaaggtc ttgtgagctt gtattttttc
     7621 tatttaagca gtgctttctc ttggactggc ttgactcatg gcattctaca cgttattgct
     7681 ggtctaaatg tgattttgcc aagcttcttc aggacctata attttgcttg acttgtagcc
     7741 aaacacaagt aaaatgatta agcaacaaat gtatttgtga agcttggttt ttaggttgtt
     7801 gtgttgtgtg tgcttgtgct ctataataat actatccagg ggctggagag gtggctcgga
     7861 gttcaagagc acagactgct cttccagaag tcctgagttc aattcccagc aaccacatgg
     7921 tggctcacaa ccatctgtaa tgggatctga tgccctcttc tggtgtgtct gaagaccaca
     7981 agtgtattca cattaaataa ataaatcctc cttcttcttc tttttttttt tttttaaaga
     8041 gaatactgtc tccagtagaa tttactgaag taatgaaata ctttgtgttt gttccaatat
     8101 ggtagccaat aatcaaatta ctctttaagc actggaaatg ttaccaagga actaattttt
     8161 atttgaagtg taactgtgga cagaggagcc ataactgcag acttgtggga tacagaagac
     8221 caatgcagac tttaatgtct tttctcttac actaagcaat aaagaaataa aaattgaact
     8281 tctagtatcc tatttgttta aactgctagc tttacttaac ttttgtgctt catctataca
     8341 aagctgaaag ctaagtctgc agccattact aaacatgaaa gcaagtaatg ataattttgg
     8401 atttcaaaaa tgtagggcca gagtttagcc agccagtggt ggtgcttgcc tttatgcctt
     8461 taatcccagc actctggagg cagagacagg cagatctctg agtttgagcc cagcctggtc
     8521 tacacatcaa gttctatcta ggatagccag gaatacacac agaaaccctg ttggggaggg
     8581 gggctctgag atttcataaa attataattg aagcattccc taatgagcca ctatggatgt
     8641 ggctaaatcc gtctaccttt ctgatgagat ttgggtatta ttttttctgt ctctgctgtt
     8701 ggttgggtct tttgacactg tgggctttct ttaaagcctc cttcctgcca tgtggtctct
     8761 tgtttgctac taacttccca tggcttaaat ggcatggctt tttgccttct aagggcagct
     8821 gctgagattt gcagcctgat ttccagggtg gggttgggaa atctttcaaa cactaaaatt
     8881 gtcctttaat ttttttttta aaaaatgggt tatataataa acctcataaa atagttatga
     8941 ggagtgaggt ggactaatat taaatgagtc cctcccctat aaaagagcta ttaaggcttt
     9001 ttgtcttata cttaactttt tttttaaatg tggtatcttt agaaccaagg gtcttagagt
     9061 tttagtatac agaaactgtt gcatcgctta atcagatttt ctagtttcaa atccagagaa
     9121 tccaaattct tcacagccaa agtcaaatta agaatttctg acttttaatg ttaatttgct
     9181 tactgtgaat ataaaaatga tagcttttcc tgaggcaggg tctcactatg tatctctgcc
     9241 tgatctgcaa caagatatgt agactaaagt tctgcctgct tttgtctcct gaatactaag
     9301 gttaaaatgt agtaatactt ttggaacttg caggtcagat tcttttatag gggacacact
     9361 aagggagctt gggtgatagt tggtaaaatg tgtttcaagt gatgaaaact tgaattatta
     9421 tcaccgcaac ctacttttta aaaaaaaaag ccaggcctgt tagagcatgc ttaagggatc
     9481 cctaggactt gctgagcaca caagagtagt tacttggcag gctcctggtg agagcatatt
     9541 tcaaaaaaca aggcagacaa ccaagaaact acagttaagg ttacctgtct ttaaaccatc
     9601 tgcatataca cagggatatt aaaatattcc aaataatatt tcattcaagt tttcccccat
     9661 caaattggga catggatttc tccggtgaat aggcagagtt ggaaactaaa caaatgttgg
     9721 ttttgtgatt tgtgaaattg ttttcaagtg atagttaaag cccatgagat acagaacaaa
     9781 gctgctattt cgaggtctct tggtttatac tcagaagcac ttctttgggt ttccctgcac
     9841 tatcctgatc atgtgctagg cctaccttag gctgattgtt gttcaaataa acttaagttt
     9901 cctgtcaggt gatgtcatat gatttcatat atcaaggcaa aacatgttat atatgttaaa
     9961 catttgtact taatgtgaaa gttaggtctt tgtgggtttg atttttaatt ttcaaaacct
    10021 gagctaaata agtcattttt acatgtctta catttggtgg aattgtataa ttgtggtttg
    10081 caggcaagac tctctgacct agtaacccta cctatagagc actttgctgg gtcacaagtc
    10141 taggagtcaa gcatttcacc ttgaagttga gacgttttgt tagtgtatac tagtttatat
    10201 gttggaggac atgtttatcc agaagatatt caggactatt tttgactggg ctaaggaatt
    10261 gattctgatt agcactgtta gtgagcattg agtggccttt aggcttgaat tggagtcact
    10321 tgtatatctc aaataatgct ggcctttttt aaaaagccct tgttctttat caccctgttt
    10381 tctacataat ttttgttcaa agaaatactt gtttggatct ccttttgaca acaatagcat
    10441 gttttcaagc catatttttt ttcctttttt tttttttttt tggtttttcg agacagggtt
    10501 tctctgtata gccctggctg tcctggaact cactttgtag accaggctgg cctcgaactc
    10561 agaaatccgc ctgcctctgc ctcctgagtg ccgggattaa aggcgtgcac caccacgcct
    10621 ggctaagttg gatattttgt tatataacta taaccaatac taactccact gggtggattt
    10681 ttaattcagt cagtagtctt aagtggtctt tattggccct tcattaaaat ctactgttca
    10741 ctctaacaga ggctgttggt actagtggca cttaagcaac ttcctacgga tatactagca
    10801 gattaagggt cagggataga aactagtcta gcgttttgta tacctaccag cgtcgacggt
    10861 atcgataagc ttgatatcga attctaccgg gtaggggagg cgcttttccc aaggcagtct
    10921 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
    10981 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
    11041 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
    11101 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
    11161 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
    11221 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
    11281 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
    11341 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcaggtcc
    11401 tcgccatgga tcctgatgat gttgttgatt cttctaaatc ttttgtgatg gaaaactttt
    11461 cttcgtacca cgggactaaa cctggttatg tagattccat tcaaaaaggt atacaaaagc
    11521 caaaatctgg tacacaagga aattatgacg atgattggaa agggttttat agtaccgaca
    11581 ataaatacga cgctgcggga tactctgtag ataatgaaaa cccgctctct ggaaaagctg
    11641 gaggcgtggt caaagtgacg tatccaggac tgacgaaggt tctcgcacta aaagtggata
    11701 atgccgaaac tattaagaaa gagttaggtt taagtctcac tgaaccgttg atggagcaag
    11761 tcggaacgga agagtttatc aaaaggttcg gtgatggtgc ttcgcgtgta gtgctcagcc
    11821 ttcccttcgc tgaggggagt tctagcgttg aatatattaa taactgggaa caggcgaaag
    11881 cgttaagcgt agaacttgag attaattttg aaacccgtgg aaaacgtggc caagatgcga
    11941 tgtatgagta tatggctcaa gcctgtgcag gaaatcgtgt caggcgatct ctttgtgaag
    12001 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
    12061 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
    12121 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcagatcct
    12181 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
    12241 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
    12301 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
    12361 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
    12421 tctatggctt ctgaggcgga aagaaccagc tggggctcga gggggggccc ggtacccagc
    12481 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
    12541 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
    12601 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
    12661 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
    12721 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
    12781 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    12841 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    12901 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    12961 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    13021 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    13081 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    13141 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    13201 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    13261 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    13321 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    13381 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    13441 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    13501 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    13561 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    13621 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    13681 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    13741 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    13801 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    13861 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    13921 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    13981 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    14041 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    14101 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    14161 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    14221 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    14281 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    14341 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    14401 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    14461 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    14521 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    14581 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    14641 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
