LOCUS       sequence-234231-       16258 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual-fluorescence reporter to monitor the expression of HIV-1 early 
            genes and late genes simultaneously..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16258)
  AUTHORS   Xiao H, Wyler E, Milek M, Grewe B, Kirchner P, Ekici A, Silva ABOV, 
            Jungnickl D, Full F, Thomas M, Landthaler M, Ensser A, Uberla K
  TITLE     CRNKL1 Is a Highly Selective Regulator of Intron-Retaining HIV-1 and
            Cellular mRNAs.
  JOURNAL   mBio. 2021 Jan 19;12(1). pii: mBio.02525-20. doi: 
            10.1128/mBio.02525-20.
  PUBMED    33468685
REFERENCE   2  (bases 1 to 16258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-01-19"; volume: "12(1). pii: mBio.02525-20. doi"; pages:
            " 10.1128/mBio.02525-20"
COMMENT     Sequence Label: HIV-dual-GT
FEATURES             Location/Qualifiers
     source          1..16258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             790..2289
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2296..3003
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     misc_feature    5624..5741
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8597..8830
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9015..9059
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9353..9379
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             9643..10317
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="DSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAKL
                     QVTKGGPLPFAWDILSPQFMYGSKVYTKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHKA
                     LKLKGGGHYLCEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERTEARHHL
                     FQ"
     primer_bind     10038..10057
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     10232..10255
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     LTR             10510..11143
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12782..12799)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12953..13541)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13033..13052)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13712..14503,14504..14572))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14335..14354
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14573..14677)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14745..14763
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14801..14823)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14923..14942
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa
     1261 gtagtagaag agaaggcttt cagcccagaa gtaataccca tgttttcagc attatcagaa
     1321 ggagccaccc cacaagattt aaataccatg ctaaacacag tggggggaca tcaagcagcc
     1381 atgcaaatgt taaaagagac catcaatgag gaagctgcag aatgggatag attgcatcca
     1441 gtgcatgcag ggcctattgc accaggccag atgagagaac caaggggaag tgacatagca
     1501 ggaactacta gtacccttca ggaacaaata ggatggatga cacataatcc acctatccca
     1561 gtaggagaaa tctataaaag atggataatc ctgggattaa ataaaatagt aagaatgtat
     1621 agccctacca gcattctgga cataagacaa ggaccaaagg aaccctttag agactatgta
     1681 gaccgattct ataaaactct aagagccgag caagcttcac aagaggtaaa aaattggatg
     1741 acagaaacct tgttggtcca aaatgcgaac ccagattgta agactatttt aaaagcattg
     1801 ggaccaggag cgacactaga agaaatgatg acagcatgtc agggagtggg gggacccggc
     1861 cataaagcaa gagttttggc tgaagcaatg agccaagtaa caaatccagc taccataatg
     1921 atacagaaag gcaattttag gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa
     1981 gaagggcaca tagccaaaaa ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga
     2041 aaggaaggac accaaatgaa agattgtact gagagacagg ctaacttcct ggggaagatc
     2101 tggccttccc acaagggaag gccagggaat tttcttcaga gcagaccaga gccaacagcc
     2161 ccaccagaag agagcttcag gtttggggaa gagacaacaa ctccctctca gaagcaggag
     2221 ccgatagaca aggaactgta tcctttagct tccctcagat cactctttgg cagcgacccc
     2281 tcgtcacaag ctagcgtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag
     2341 ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc
     2401 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc
     2461 ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac
     2521 acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga
     2581 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac
     2641 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg
     2701 atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc
     2761 ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca
     2821 aacgccaaga ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc
     2881 tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag
     2941 cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt
     3001 aatagccatg gcttcccgcc ggcggtggcg gcgcaggatg atggcacgct gcccatgtct
     3061 tgtgcccagg agagcgggat ggaccgtcac cctgcagcct gtgcttctgc taggatcaat
     3121 gtgtaatagt ctagaagata ggggggcaat taaaggaagc tctattagat acaggagcag
     3181 atgatacagt attagaagaa atgaatttgc caggaagatg gaaaccaaaa atgatagggg
     3241 gaattggagg ttttatcaaa gtaagacagt atgatcagat actcatagaa atctgcggac
     3301 ataaagctat aggtacagta ttagtaggac ctacacctgt caacataatt ggaagaaatc
     3361 tgttgactca gattggctgc actttaaatt ttcccattag tcctattgag actgtaccag
     3421 taaaattaaa gccaggaatg gatggcccaa aagttaaaca atggccattg acagaagaaa
     3481 aaataaaagc attagtagaa atttgtacag aaatggaaaa ggaaggaaaa atttcaaaaa
     3541 ttgggcctga aaatccatac aatactccag tatttgccat aaagaaaaaa gacagtacta
     3601 aatggagaaa attagtagat ttcagagaac ttaataagag aactcaagat ttctgggaag
     3661 ttcaattagg aataccacat cctgcagggt taaaacagaa aaaatcagta acagtactgg
     3721 atgtgggcga tgcatatttt tcagttccct tagataaaga cttcaggaag tatactgcat
     3781 ttaccatacc tagtataaac aatgagacac cagggattag atatcagtac aatgtgcttc
     3841 cacagggatg gaaaggatca ccagcaatat tccagtgtag catgacaaaa atcttagagc
     3901 cttttagaaa acaaaatcca gacatagtca tctatcaata catggatgat ttgtatgtag
     3961 gatctgactt agaaataggg cagcatagaa caaaaataga ggaactgaga caacatctgt
     4021 tgaggtgggg atttaccaca ccagacaaaa aacatcagaa agaacctcca ttcctttgga
     4081 tgggttatga actccatcct gataaatgga cagtacagcc tatagtgctg ccagaaaagg
     4141 acagctggac tgtcaatgac atacagaaat tagtgggaaa attgaattgg gcaagtcaga
     4201 tttatgcagg gattaaagta aggcaattat gtaaacttct taggggaacc aaagcactaa
     4261 cagaagtagt accactaaca gaagaagcag agctagaact ggcagaaaac agggagattc
     4321 taaaagaacc ggtacatgga gtgtattatg acccatcaaa agacttaata gcagaaatac
     4381 agaagcaggg gcaaggccaa tggacatatc aaatttatca agagccattt aaaaatctga
     4441 aaacaggaaa atatgcaaga atgaagggtg cccacactaa tgatgtgaaa caattaacag
     4501 aggcagtaca aaaaatagcc acagaaagca tagtaatatg gggaaagact cctaaattta
     4561 aattacccat acaaaaggaa acatgggaag catggtggac agagtattgg caagccacct
     4621 ggattcctga gtgggagttt gtcaataccc ctcccttagt gaagttatgg taccagttag
     4681 agaaagaacc cataatagga gcagaaactt tctatgtaga tggggcagcc aatagggaaa
     4741 ctaaattagg aaaagcagga tatgtaactg acagaggaag acaaaaagtt gtccccctaa
     4801 cggacacaac aaatcagaag actgagttac aagcaattca tctagctttg caggattcgg
     4861 gattagaagt aaacatagtg acagactcac aatatgcatt gggaatcatt caagcacaac
     4921 cagataagag tgaatcagag ttagtcagtc aaataataga gcagttaata aaaaaggaaa
     4981 aagtctacct ggcatgggta ccagcacaca aaggaattgg aggaaatgaa caagtagatg
     5041 ggttggtcag tgctggaatc aggaaagtac tatttttaga tggaatagat aaggcccaag
     5101 aagaacatga gaaatatcac agtaattgga gagcaatggc tagtgatttt aacctaccac
     5161 ctgtagtagc aaaagaaata gtagccagct gtgataaatg tcagctaaaa ggggaagcca
     5221 tgcatggaca agtagactgt agcccaggaa tatggcagct agattgtaca catttagaag
     5281 gaaaagttat cttggtagca gttcatgtag ccagtggata tatagaagca gaagtaattc
     5341 cagcagagac agggcaagaa acagcatact tcctcttaaa attagcagga agatggccag
     5401 taaaaacagt acatacagac aatggcagca atttcaccag tactacagtt aaggccgcct
     5461 gttggtgggc ggggatcaag caggaatttg gcattcccta caatccccaa agtcaaggag
     5521 taatagaatc tatgaataaa gaattaaaga aaattatagg acaggtaaga gatcaggctg
     5581 aacatcttaa gacagcagta caaatggcag tattcatcca caattttaaa agaaaagggg
     5641 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     5701 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     5761 gcagagatcc agtttggaaa ggaccagcaa agctcctctg gaaaggtgaa ggggcagtag
     5821 taatacaaga taatagtgac ataaaagtag tgccaagaag aaaagcaaag atcatcaggg
     5881 attatggaaa acagatggca ggtgatgatt gtgtggcaag tagacaggat gaggattaac
     5941 acatggaaaa gattagtaaa acaccatatg tagatttcaa ggaaagctaa ggactggttt
     6001 tatagacatc actatgaaag tactaatcca aaaataagtt cagaagtaca catcccacta
     6061 ggggatgcta aattagtaat aacaacatat tggggtctgc atacaggaga aagagactgg
     6121 catttgggtc agggagtctc catagaatgg aggaaaaaga gatatagcac acaagtagac
     6181 cctgacctag cagaccaact aattcatctg cactattttg attgtttttc agaatctgct
     6241 ataagaaata ccatattagg acgtatagtt agtcctaggt gtgaatatca agcaggacat
     6301 aacaaggtag gatctctaca gtacttggca ctagcagcat taataaaacc aaaacagata
     6361 aagccacctt tgcctagtgt taggaaactg acagaggaca gatggaacaa gccccagaag
     6421 actaagggcc acagagggag ccatacaatg aatggacact agagctttta gaggaactta
     6481 agagtgaagc tgttagacat tttcctagga tatggctcca taacttagga caacatatct
     6541 atgaaactta cggggatact tgggcaggag tggaagccat aataagaatt ctgcaacaac
     6601 tgctgtttat ccatttcaga attgggtgtc gacatagcag aataggcgtt actcgacaga
     6661 ggagagcaag aaatggagcc agtagatcct agactagagc cctggaagca tccaggaagt
     6721 cagcctaaaa ctgcttgtac caattgctat tgtaaaaagt gttgctttca ttgccaagtt
     6781 tgtttcatga caaaagcctt aggcatctcc tacggcagga agaagcggag acagcgacga
     6841 agagctcatc agaacagtca gactcatcaa gcttctctaa caaagcagta agtagtacat
     6901 gtaatgcaac cttaatagta gcaatagtag cattagtagt agcaataata atagcaatag
     6961 ttgtgtggtc catagtaatc atagaatata ggaaaatatt aagacaaaga aaaatagaca
     7021 ggttaattga tagactaata gaaagagcag aagacagtgg caatgagagt gaaggagaag
     7081 tatcagcact tgtggagatg ggggtggaaa tggggcacca tgcgccgtgg gacatcgatg
     7141 atctgtagtg ctacagaaaa attgtgggtc acagtctatt atggggtacc tgtgtggaag
     7201 gaagcaacca ccactctatt ttgtgcatca gatgctaaag catatgatac agaggtacat
     7261 aatgtttggg ccacacatgc ctgtgtaccc acagacccca acccacaaga agtagtattg
     7321 gtaaatgtga cagaaaattt taacatgtgg aaaaatgaca tggtagaaca gatgcatgag
     7381 gatataatca gtttatggga tcaaagccta aagccatgtg taaaattaac cccactctgt
     7441 gttagtttaa agtgcactga tttgaagaat gatactaata ccaatagtag tagcgggaga
     7501 atgataatgg agaaaggaga gataaaaaac tgctctttca atatcagcac aagcataaga
     7561 gataaggtgc agaaagaata tgcattcttt tataaacttg atatagtacc aatagataat
     7621 accagctata ggttgataag ttgtaacacc tcagtcatta cacaggcctg tccaaaggta
     7681 tcctttgagc caattcccat acattattgt gccccggctg gttttgcgat tctaaaatgt
     7741 aataataaga cgttcaatgg aacaggacca tgtacaaatg tcagcacagt acaatgtaca
     7801 catggaatca ggccagtagt atcaactcaa ctgctgttaa atggcagtct agcagaagaa
     7861 gatgtagtaa ttagatctgc caatttcaca gacaatgcta aaaccataat agtacagctg
     7921 aacacatctg tagaaattaa ttgtacaaga cccaacaaca atacaagaaa aagtatccgt
     7981 atccagaggg gaccagggag agcatttgtt acaataggaa aaataggaaa tatgagacaa
     8041 gcacattgta acattagtag agcaaaatgg aatgccactt taaaacagat agcaaattaa
     8101 gagaacaatt tggaaataat aaaacaataa tctttaagca atcctcagga ggggacccag
     8161 aaattgtaac gcacagtttt aattgtggag gggaattttt ctactgtaat tcaacacaac
     8221 tgtttaatag tacttggttt aatagtactt ggagtactga agggtcaaat aacactgaag
     8281 gaagtgacac aatcacactc ccatgcagaa taaaacaatt tataaacatg tggcaggaag
     8341 taggaaaagc aatgtatgcc cctcccatca gcggacaaat tagatgttca tcaaatatta
     8401 cagggctgct attaacaaga gatggtggta ataacaacaa tgggtccgag atcttcagac
     8461 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     8521 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     8581 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     8641 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     8701 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     8761 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     8821 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     8881 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     8941 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     9001 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     9061 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     9121 gaggcttggt aggtttaaga atagtttttg ctgtactttc tgtagtgaat agagttaggc
     9181 agggatattc accattatcg tttcagaccc acctcccaat cccgagggga cccgacaggc
     9241 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     9301 acggatcctt agcacttatc tgggacgatc tgcggagcct gtgcctcttc agctaccacc
     9361 gcttgagaga cttactcttg attgtaacga ggattgtgga acttctggga cgcagggggt
     9421 gggaagccct caaatattgg tggaatctcc tacagtattg gagtcaggaa ctaaagaata
     9481 gtgctgttaa cttgctcaat gccacagcca tagcagtagc tgaggggaca gatagggtta
     9541 tagaagtatt acaagcagct tatagagcta ttcgccacat acctagaaga ataagacagg
     9601 gcttggaaag gattttgcta taagatgggt ggcgcggccg ctgatagcac tgagaacgtc
     9661 atcaagccct tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     9721 gagatcgagg gcgagggcga gggcaagccc tacgagggca cccagaccgc caagctgcag
     9781 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac
     9841 ggctccaagg tgtacaccaa gcaccccgcc gacatccccg actacaagaa gctgtccttc
     9901 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     9961 acccaggact cctccctgca ggacggcacc ttcatctacc acgtgaagtt catcggcgtg
    10021 aacttcccct ccgacggccc cgtaatgcag aagaagactc tgggctggga gccctccacc
    10081 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccacaaggc gctgaagctg
    10141 aagggcggcg gccactacct gtgtgagttc aagtcaatct acatggccaa gaagcccgtg
    10201 aagctgcccg gctactacta cgtggactcc aagctggaca tcacctccca caacgaggac
    10261 tacaccgtgg tggagcagta cgagcgcacc gaggcccgcc accacctgtt ccagtagtaa
    10321 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct aacaatgctg
    10381 cttgtgcctg gctagaagca caagaggagg aagaggtggg ttttccagtc acacctcagg
    10441 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
    10501 aggggggact ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
    10561 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccaggggtca
    10621 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
    10681 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catggaatgg
    10741 atgaccctga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
    10801 tggcccgaga gctgcatccg gagtacttca agaactgctg acatcgagct tgctacaagg
    10861 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
    10921 ccctcagatg ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
    10981 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    11041 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    11101 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcacccagga ggtagaggtt
    11161 gcagtgagcc aagatcgcgc cactgcattc cagcctgggc aagaaaacaa gactgtctaa
    11221 aataataata ataagttaag ggtattaaat atatttatac atggaggtca taaaaatata
    11281 tatatttggg ctgggcgcag tggctcacac ctgcgcccgg ccctttggga ggccgaggca
    11341 ggtggatcac ctgagtttgg gagttccaga ccagcctgac caacatggag aaaccccttc
    11401 tctgtgtatt tttagtagat tttattttat gtgtatttta ttcacaggta tttctggaaa
    11461 actgaaactg tttttcctct actctgatac cacaagaatc atcagcacag aggaagactt
    11521 ctgtgatcaa atgtggtggg agagggaggt tttcaccagc acatgagcag tcagttctgc
    11581 cgcagactcg gcgggtgtcc ttcggttcag ttccaacacc gcctgcctgg agagaggtca
    11641 gaccacaggg tgagggctca gtccccaaga cataaacacc caagacataa acacccaaca
    11701 ggtccacccc gcctgctgcc caggcagagc cgattcacca agacgggaat taggatagag
    11761 aaagagtaag tcacacagag ccggctgtgc gggagaacgg agttctatta tgactcaaat
    11821 cagtctcccc aagcattcgg ggatcagagt ttttaaggat aacttagtgt gtagggggcc
    11881 agtgagttgg agatgaaagc gtagggagtc gaaggtgtcc ttttgcgccg agtcagttcc
    11941 tgggtggggg ccacaagatc ggatgagcca gtttatcaat ccgggggtgc cagctgatcc
    12001 atggagtgca gggtctgcaa aatatctcaa gcactgattg atcttaggtt ttacaatagt
    12061 gatgttaccc caggaacaat ttggggaagg tcagaatctt gtagcctgta gctgcatgac
    12121 tcctaaacca taatttcttt tttgtttttt tttttttatt tttgagacag ggtctcactc
    12181 tgtcacctag gctggagtgc agtggtgcaa tcacagctca ctgcagcctc aacgtcgtaa
    12241 gctcaagcga tcctcccacc tcagcctgcc tggtagctga gactacaagc gacgccccag
    12301 ttaatttttg tatttttggt agaggcagcg ttttgccgtg tggccctggc tggtctcgaa
    12361 ctcctgggct caagtgatcc agcctcagcc tcccaaagtg ctgggacaac cgggcccagt
    12421 cactgcacct ggccctaaac cataatttct aatcttttgg ctaatttgtt agtcctacaa
    12481 aggcagtcta gtccccagca aaaagggggt ttgtttcggg aaagggctgt tactgtcttt
    12541 gtttcaaact ataaactaag ttcctcctaa acttagttcg gcctacaccc aggaatgaac
    12601 aaggagagct tggaggttag aagcacgatg gaattggtta ggtcagatct ctttcactgt
    12661 ctgagttata attttgcaat ggtggttcaa agactgcccg cttctgacac cagtcgctgc
    12721 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    12781 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    12841 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    12901 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    12961 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    13021 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    13081 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    13141 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    13201 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    13261 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    13321 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    13381 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    13441 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    13501 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    13561 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    13621 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    13681 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    13741 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    13801 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    13861 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    13921 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    13981 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    14041 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    14101 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    14161 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    14221 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    14281 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    14341 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    14401 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    14461 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    14521 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    14581 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    14641 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    14701 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    14761 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    14821 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    14881 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
    14941 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    15001 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    15061 cctcttcgct attacgccag gggaggcaga gattgcagta agctgagatc gcagcactgc
    15121 actccagcct gggcgacaga gtaagactct gtctcaaaaa taaaataaat aaatcaatca
    15181 gatattccaa tcttttcctt tatttattta tttattttct attttggaaa cacagtcctt
    15241 ccttattcca gaattacaca tatattctat ttttctttat atgctccagt tttttttaga
    15301 ccttcacctg aaatgtgtgt atacaaaatc taggccagtc cagcagagcc taaaggtaaa
    15361 aaataaaata ataaaaaata aataaaatct agctcactcc ttcacatcaa aatggagata
    15421 cagctgttag cattaaatac caaataaccc atcttgtcct caataatttt aagcgcctct
    15481 ctccaccaca tctaactcct gtcaaaggca tgtgcccctt ccgggcgctc tgctgtgctg
    15541 ccaaccaact ggcatgtgga ctctgcaggg tccctaactg ccaagcccca cagtgtgccc
    15601 tgaggctgcc ccttccttct agcggctgcc cccactcggc tttgctttcc ctagtttcag
    15661 ttacttgcgt tcagccaagg tctgaaacta ggtgcgcaca gagcggtaag actgcgagag
    15721 aaagagacca gctttacagg gggtttatca cagtgcaccc tgacagtcgt cagcctcaca
    15781 gggggtttat cacattgcac cctgacagtc gtcagcctca cagggggttt atcacagtgc
    15841 acccttacaa tcattccatt tgattcacaa tttttttagt ctctactgtg cctaacttgt
    15901 aagttaaatt tgatcagagg tgtgttccca gaggggaaaa cagtatatac agggttcagt
    15961 actatcgcat ttcaggcctc cacctgggtc ttggaatgtg tcccccgagg ggtgatgact
    16021 acctcagttg gatctccaca ggtcacagtg acacaagata accaagacac ctcccaaggc
    16081 taccacaatg ggccgccctc cacgtgcaca tggccggagg aactgccatg tcggaggtgc
    16141 aagcacacct gcgcatcaga gtccttggtg tggagggagg gaccagcgca gcttccagcc
    16201 atccacctga tgaacagaac ctagggaaag ccccagttct acttacacca ggaaaggc
//
