LOCUS       sequence-234342-         324 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express M.EcoGII fused to TRF1 with detabilization domain - 
            inducible.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Sobecki M, Souaid C, Boulay J, Guerineau V, Noordermeer D, Crabbe L
  TITLE     MadID, a Versatile Approach to Map Protein-DNA Interactions, 
            Highlights Telomere-Nuclear Envelope Contact Sites in Human Cells.
  JOURNAL   Cell Rep. 2018 Dec 4;25(10):2891-2903.e5. doi: 
            10.1016/j.celrep.2018.11.027.
  PUBMED    30517874
REFERENCE   2  (bases 1 to 324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.11"; journalName: "Cell Rep"; date: 
            "2018-12-4- 4"; volume: "25"; issue: "10"; pages: "2891-2903.e5"
COMMENT     Sequence Label: pRetroX-PTuner DD-M.EcoGII-v5-Telomeric
            repeat...
FEATURES             Location/Qualifiers
     source          1..324
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..324)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
ORIGIN
        1 atgggagtgc aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc
       61 cagacctgtg tggtgcacta caccgggatg cttgaagatg gaaagaaagt cgattcctcc
      121 cgggacagaa acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg
      181 gaagaagggg ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat
      241 tatgcctatg gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc
      301 gatgtggagc ttctaaaacc ggaa
//
