LOCUS       sequence-234367-        4098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expresses the phosphopantheteine transferase Sfp of Bacillus 
            subtilis to transfer a PPant arm to acyl carrier proteins in 
            Escherichia coli. Untagged; in pCDF-1b vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Rittner A, Paithankar KS, Drexler DJ, Himmler A, Grininger M
  TITLE     Probing the modularity of megasynthases by rational engineering of a
            fatty acid synthase Type I.
  JOURNAL   Protein Sci. 2019 Feb;28(2):414-428. doi: 10.1002/pro.3550. Epub 
            2018 Dec 20.
  PUBMED    30394635
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.3550"; 
            journalName: "Protein Sci"; date: "2019-02"; volume: "28"; issue: 
            "2"; pages: "414-428"
COMMENT     Sequence Label: pAR357_SFP_pCDF-1b
FEATURES             Location/Qualifiers
     source          1..4098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(695..713)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      709..756
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(927..1718)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1719..1810)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1858..2596)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(2806..3888)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3850..3869
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3889..3966)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4012..4031
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4012..4030
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4031..4055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgaagattt acggaattta tatggaccgc ccgctttcac aggaagaaaa tgaacggttc
       61 atgactttca tatcacctga aaaacgggag aaatgccgga gattttatca taaagaagat
      121 gctcaccgca ccctgctggg agatgtgctc gttcgctcag tcataagcag gcagtatcag
      181 ttggacaaat ccgatatccg ctttagcacg caggaatacg ggaagccgtg catccctgat
      241 cttcccgacg ctcatttcaa catttctcac tccggccgct gggtcattgg tgcgtttgat
      301 tcacagccga tcggcataga tatcgaaaaa acgaaaccga tcagccttga gatcgccaag
      361 cgcttctttt caaaaacaga gtacagcgac cttttagcaa aagacaagga cgagcagaca
      421 gactattttt atcatctatg gtcaatgaaa gaaagcttta tcaaacagga aggcaaaggc
      481 ttatcgcttc cgcttgattc cttttcagtg cgcctgcatc aggacggaca agtatccatt
      541 gagcttccgg acagccattc cccatgctat atcaaaacgt atgaggtcga tcccggctac
      601 aaaatggctg tatgcgccgc acaccctgat ttccccgagg atatcacaat ggtctcgtac
      661 gaagagcttt tataattaac ctaggctgct gccaccgctg agcaataact agcataaccc
      721 cttggggcct ctaaacgggt cttgaggggt tttttgctga aacctcaggc atttgagaag
      781 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg
      841 gctatttaac gaccctgccc tgaaccgacg accgggtcat cgtggccgga tcttgcggcc
      901 cctcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc
      961 acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt
     1021 ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc
     1081 attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa
     1141 atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc
     1201 catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag
     1261 agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag
     1321 atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc
     1381 tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg
     1441 tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa
     1501 gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc
     1561 gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac
     1621 aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat
     1681 agttgagtcg atacttcggc gatcaccgct tccctcatac tcttcctttt tcaatattat
     1741 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     1801 aataaacaaa tagctagctc actcggtcgc tacgctccgg gcgtgagact gcggcgggcg
     1861 ctgcggacac atacaaagtt acccacagat tccgtggata agcaggggac taacatgtga
     1921 ggcaaaacag cagggccgcg ccggtggcgt ttttccatag gctccgccct cctgccagag
     1981 ttcacataaa cagacgcttt tccggtgcat ctgtgggagc cgtgaggctc aaccatgaat
     2041 ctgacagtac gggcgaaacc cgacaggact taaagatccc caccgtttcc ggcgggtcgc
     2101 tccctcttgc gctctcctgt tccgaccctg ccgtttaccg gatacctgtt ccgcctttct
     2161 cccttacggg aagtgtggcg ctttctcata gctcacacac tggtatctcg gctcggtgta
     2221 ggtcgttcgc tccaagctgg gctgtaagca agaactcccc gttcagcccg actgctgcgc
     2281 cttatccggt aactgttcac ttgagtccaa cccggaaaag cacggtaaaa cgccactggc
     2341 agcagccatt ggtaactggg agttcgcaga ggatttgttt agctaaacac gcggttgctc
     2401 ttgaagtgtg cgccaaagtc cggctacact ggaaggacag atttggttgc tgtgctctgc
     2461 gaaagccagt taccacggtt aagcagttcc ccaactgact taaccttcga tcaaaccacc
     2521 tccccaggtg gttttttcgt ttacagggca aaagattacg cgcagaaaaa aaggatctca
     2581 agaagatcct ttgatctttt ctactgaacc gctctagatt tcagtgcaat ttatctcttc
     2641 aaatgtagca cctgaagtca gccccatacg atataagttg taattctcat gttagtcatg
     2701 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat
     2761 cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc
     2821 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2881 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
     2941 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
     3001 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
     3061 ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat
     3121 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
     3181 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
     3241 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
     3301 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
     3361 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
     3421 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
     3481 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
     3541 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
     3601 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
     3661 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
     3721 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     3781 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
     3841 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     3901 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     3961 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaaa ttaatacgac
     4021 tcactatagg ggaattgtga gcggataaca attcccctgt agaaataatt ttgtttaact
     4081 ttaataagga gatatacc
//
