LOCUS       sequence-234381-       10104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 10104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_164760-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..10104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4882..4899
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4951..5006
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5005..5024)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7242..7260)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7328..7432
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7433..7501,7502..8293)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7651..7670)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8464..9052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8953..8972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9206..9223
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9340..9361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9376..9381,9382..9399,9400..9406)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9414..9430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9419..9441
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9438..9454
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9438..9454
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        9725..10104
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccagcttct acttccggac caaggacatc
      841 tgcaacgaca gagagaagat cagcatcatc ggcggcgagc agcggcggct ggtcacagag
      901 ggtggcagag tgaccctgat ctgctgcgtg cccggcaaga gcaacggcca cgaccacgag
      961 cagctcgtgt ggaacagccc cgacggcaaa gagctggtca actacttcag caaccccatc
     1021 agcgacaacc agcacaccgc ctacagcgtg cccgacttcc acagcaagga ccacctgatc
     1081 accatgatgg tcatccagaa gttccagacc aaggatagcg gccagtacac ctgtcggaac
     1141 acccacccca gcagcaccgc cctgcctgcc agcgtgttca tcgacgtgcg gcccagactg
     1201 ctggccgact tcatccccct gccccccagc aacgtggaag atagcggatc tgccgtggtg
     1261 gccggcgtgg ccgtgtctgt ggctctggaa gagggcagac ggggcgagat cacctgtaga
     1321 gtgaacagcc agctgaacct gggcgacgtg cagctgacat ggttttttca gggccggcag
     1381 ctgatcaccc ccagcatcgt gatgaagcag atcggccaga agaacctgtt caagatcaac
     1441 aacaccagcc acgagaaccc ccccaccgaa gtgggcgaca gcgacatctt cgtgcccagc
     1501 agccacgagc ggaacttcgt gtccggcagc aaccagctgt tcaccaagca gctgaaccgg
     1561 cccgtggccc tggaccccga ggaactgggc atccagatca gccagaacgg ccagatcctg
     1621 gacatcagcc gggtggacac ccggcacagc ggcatctttc tgtgcaaggc cgaggccgtg
     1681 aaccccacct ggtcccccca gaacagcgag tacgacagca gactgtacga gagcggcgtc
     1741 gtgaccgccc cattcctgat ccagatccag gccatccggg ccatcgtgta cagcagaccc
     1801 aggctgctgc ccggctcccc cgtggaaatc ttcagcacca ccgacagcga cgagagccag
     1861 ctggtggacc cctacaagag attcgtggac aagcggcagg acgagcagag actggacacc
     1921 atccagggca gccccaacta cctgcagacc gtgctgcccc tgtggcgcgg agacaaagag
     1981 aaagaaaagc ccttcatgac caacgcccgg aagatggtgc aggaaggcgg cagactgatc
     2041 ctggaatgcc aggccagagg cagacccagc ccccagctgc tgtggtacag aggcggcatg
     2101 ggcagcagca ccaccaactc cagccaggcc gtgaccttcg accagcggat caagagcgcc
     2161 ctgcagcatc tgcccctgga agatgtgcag agagagctgg gaatcctgat cgccgacttc
     2221 aaccacctgt tccccgccgt gatcaacatg gtcaacatca gcaacgacgt gaccaacaag
     2281 gcccccgaga accccggcag catccaccgg aagggaatcc agacctacga gctgggcaga
     2341 ttccagctga gcaccggcct gcggaaggac gacagaggcg accccgtgat cgccatgagc
     2401 cggctgacca tcaacaacct gatgcccagc gacgccaccc ggtacacatg cctggccctg
     2461 ctgaacctga actactacgg cggcgtgggc aacttcaccg acgtgggcag catcctgccc
     2521 gacgtggtgc tgaggcccag attcgtgcgg agcggcacca acctgaaggc cagcggctac
     2581 cccggcgaga gctgcagact gacatgcgag gcctacggcg gcctgcccaa cgagatggga
     2641 ctgagagtgc ggctgctgaa gggccctggc gtgaagaagc tgatctccta cctgagctac
     2701 agcatgtaca gcgagaacaa gaaccccatg ttcaagagct acgaggacct gatcgagccc
     2761 cagagcgtgc acagcagcta caccaaccct atgttcaacc acaagagctt cgtgaacggc
     2821 aacgagctga gcgactggtt cccctccgac atctggtaca ccagcctgct gaacggcgac
     2881 ggcatgtccg acaagagcaa caacagcgac ttcatcgaga tcgtgaagcc cggcatgaac
     2941 cccagatacc acctgaaggt ggaacccgac agccggaacc cctacgccac catcctgcgg
     3001 ctggacatca ccgacctgac ccccgaggac aacagcttct acgcctgcga ggccctgagc
     3061 ggccctcatt ggagagccgt gacccctacc agcagcgagc cccctgggag agtgaccgtg
     3121 tggttcgccc ctcccgtggc cgagcccaga agaatcagcg agatcagcga ccggatggac
     3181 gccaacgaga gcgagacact gagccagccc ggcaaatacg tggtgtacgg cctgagccat
     3241 ctgcccagca agatcgcctg tcaggccctg ggccagccca ccccccagtg gaaatgggtc
     3301 ggacctcaga gcggccctag cgtgcccctg ggcgagctga acgaccccaa gggctacatc
     3361 aagatggact accaggacgg cgagtacagc gtgaccgagc tgaccgtgcc tgccggctac
     3421 tggaacgtgg gcgtgttcgg cacctacagc tgcaccgcca ccaacagact gggccacgcc
     3481 acaggacacg tgcggctgca gctggcctct caccctggca gacctgccct gcgggcctgc
     3541 agagtggaag ccacactgat cgagctgtgc gtggaacccc ccaacgagac aggcggactg
     3601 cccctgaccc actacgagct gaggatccag acccacccta gaggcgcctt ccacggcccc
     3661 ttcgcctacc tgcctggcag aagagtgctg cggctgccca acctgatccc cggctactac
     3721 tacagattcg ccctgagcgc cgtgtccaga gccggcagag gccctaatgc ctacctgcag
     3781 gtcacaacaa accggctgag cagacccgtg atcaagctgc tggccagcca ggactacatc
     3841 agccccaccg gctacaacgt gcactggaac accgaaacca ccaacggcat ggacatcaac
     3901 cagtacaaca tcaacatcag gcccgtggaa gtggactaca gcatgggcga gatcgccggc
     3961 agacccatcg gcagctggac ccacttcaac aacatcgagc ccctgtgcgt gaccagcgtg
     4021 gccgaggatc agaagatctg caacttcctg atcgacgacc tgaagcccaa cagcgcctat
     4081 gagctggaac tgagcggcca gaactccatg ggcttcagcg agacacagcg gatcatcttc
     4141 agaaccagca gcctgaccgg cgtgaacgga cggatcctga acatgcccag caccatgagc
     4201 ctgaagggca agtccaacca cgtgcaccct ggcgcgccgt cgacctccat cacggcctat
     4261 aagagtgagg gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg
     4321 cagggagagt tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc
     4381 tccctaaaga accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg
     4441 tccgaaacgc tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct
     4501 ggcaacctga ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg
     4561 atgaaagtga ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca
     4621 cccaagatga gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag
     4681 aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt
     4741 gaagaggtca agatggactc caagatccag gttttatcca aagggttgaa ttccggcagc
     4801 ctgcaccaca tcctggacgc ccagaagatg ctgtggaacc acagggacag gaacctgcct
     4861 cctctggccc ctctgggccc ccaccaccat caccaccatt gagtctagac acgtgattaa
     4921 ttaaggatcc cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag
     4981 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg
     5041 tcgagatccc tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc
     5101 tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt
     5161 ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa
     5221 ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga
     5281 gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta
     5341 tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc
     5401 ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat
     5461 aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa
     5521 acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat
     5581 agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta
     5641 aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga
     5701 ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg
     5761 gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa
     5821 taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt
     5881 gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca
     5941 atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa
     6001 tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct
     6061 cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat
     6121 taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta
     6181 acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc
     6241 ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc
     6301 ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt
     6361 tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt
     6421 caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg
     6481 ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg
     6541 cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt
     6601 gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc
     6661 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     6721 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
     6781 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     6841 taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc
     6901 taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     6961 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     7021 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     7081 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     7141 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat
     7201 gataagctgt caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt
     7261 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     7321 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7381 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7441 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7501 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7561 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7621 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     7681 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7741 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7801 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7861 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7921 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     7981 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8041 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8101 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8161 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8221 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8281 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8341 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8401 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8461 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8521 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8581 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8641 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8701 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8761 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8821 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8881 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8941 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9001 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9061 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9121 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9181 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     9241 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     9301 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     9361 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     9421 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga
     9481 ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca
     9541 ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa
     9601 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     9661 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
     9721 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     9781 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     9841 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     9901 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     9961 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
    10021 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
    10081 gtattagtca tcgctattac catg
//
