LOCUS       sequence-234384-        9156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 9156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_171460-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..9156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3934..3951
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4003..4058
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4057..4076)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6294..6312)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6380..6484
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6485..6553,6554..7345)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6703..6722)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7516..8104
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8005..8024
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8258..8275
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8392..8413
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8428..8433,8434..8451,8452..8458)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8466..8482
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8471..8493
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8490..8506
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8490..8506
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        8777..9156
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccctgaaca tcaaagaggt ggacgtcgac
      841 ggcaactact acgtgcagtg gggcgacaac ctggccctga cctgcgccca ggataccccc
      901 ccattcggca aggccagcat cgactggttt ctgcccagcc agctgaaccg gcccgtgggc
      961 tatggcaaca aggcccacgt gtaccggcag gaccagctgg acagcatggt gctggtgctg
     1021 gtcgtgcgga gcgtgaacac cgacgacagc ggcacctaca cctgtcggac cggcagacag
     1081 gaaggcaacc agttcatcga gatgggccgg aagtccgtga acgtgatcgt gcggaagtcc
     1141 atcgtggcca ccgactgccc cagggaccag tggatccctg tgtccggcga gatcaccaac
     1201 tgctggggca acggcaccga gtgcggcgtg ccaacagcca ccgtgcggtg catcatcgag
     1261 gccttccctg cccccaccat ccactggcgg ttcaagggcg tgcagctgac caccggcacc
     1321 aagtacatca tcaccacctt cggcatcacc atcatcaacc ccaccaccga ggactccggc
     1381 atctacaccg tgatcgcccg gcagcctcag cagaccgccg tgttcgacct gcagatcagc
     1441 gccttcagcc ggcccagaat caccagcggc cctagcatcg tgcggaccca caacaacacc
     1501 ttcgtgtccg gcagagaagc ctacctgcag tgcctggcca gcggccagcc tccccctaca
     1561 atccactggt atcacgagcg ggacccccag accgagctgc agaaggccaa ccccaagaaa
     1621 ttctccgtga acaccaactt cagagtgggc ctgctgtgga tcagcgaggt gtcctacccc
     1681 gaggatagcg gcagctacat ctgcagagcc gtgatccccg tgcccgccac ctacagcgat
     1741 agcaccctgg tcaccaccac agaggcccag atccccatcg aagtgaccct gccccccacc
     1801 ctgatccccc tgaccaccat gcacagctac gtggaactgg gcggcaccgc caccgtgcag
     1861 tgtagagtgc gggccaccac ccccctggaa ctgtacttca agcggttcaa cagcagcacc
     1921 ccctacatca gcggcgtgca gcccggcgac aagcggatca gagtgtggcg cgagggcgac
     1981 atgaacgacc ccctgaacca cgacctgttt ctgaccatcg agaacaccac catcgacgac
     2041 acctggaact acagctgcca cgccgtgaac cagggcaaca gcagctggtg gaataccacc
     2101 atccaggtca tccagacccc ccagatcctg ctgcacagca ccctgaacac cgatagcgag
     2161 ctgagcggcc tgagattcgg ctggcgctac cagggcacca acctgacctg catcagccac
     2221 ggcatgcccc accccacctg gacctggtac agacggggcg agcagatcct gaacggccag
     2281 aacctgacct tcgtgatcat cagctacgac tactgggacc acagccagag ctggctgcag
     2341 atcaccccct gcctgtacac cgagcacttc atctacgacg actacgtgtg caaggccacc
     2401 aacatcaagg gcaccaatga gagcaaggtg tccttcagac gggccagcgt gcccggccag
     2461 cccagactgg aaagctacag cgtgacccag agcaccatct ttctgaacgt gtcccccccc
     2521 atcaacaccg gcggcatgcc taccctggcc tacgagttca cctaccgggc ctttggcggc
     2581 cctgaggggt ggtacggccc tgtgacatac cccctggacg gcagcagaag cagcggcaag
     2641 cccaagttcg agctgacagg cctgctgggc gacaccaact accagctgaa gctgagcgcc
     2701 tgctctatcg tgggcagagg catcccctac agcttcagca ccaccaccgc cagcagcacc
     2761 agacccggcc ctgtggaagt gatccacagc gccacaggca tctaccccta cggccacgtg
     2821 gtcaactgga tcccccctat gagcggcggc tcccccatcc tgggctacag aatcagagtc
     2881 agaagcgtgg aaaacgccct gaacctgatc agcatggtgg aaaacatcag ccccagcagc
     2941 agctggaagg tgtacacccc cagcttcaac aacccctaca tggactacta ccacctggcc
     3001 cccctgaagc ccgaccagac ataccagctg atcgtggaag cctacaaccg gcacggctac
     3061 agcctggacg gcgtggacat cgagcagtac ggctacctga accggacccc caccagcacc
     3121 ccccagctgc agcagatctt ccccggcgag cggctgaact accagaacag cgacaagctg
     3181 agcaacagcg tgaacagcct gatgaacttc aagcagagcg acctggtgcc catctggtat
     3241 ctgttcgcca cccccagcgc cgacgagctg gaccctcctt ctggcgcgcc gtcgacctcc
     3301 atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga
     3361 gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc
     3421 tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc
     3481 aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag
     3541 tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg
     3601 aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg
     3661 ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc
     3721 tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta
     3781 ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg
     3841 aattccggca gcctgcacca catcctggac gcccagaaga tgctgtggaa ccacagggac
     3901 aggaacctgc ctcctctggc ccctctgggc ccccaccacc atcaccacca ttgagtctag
     3961 acacgtgatt aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt
     4021 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     4081 caaatcattt ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg
     4141 acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc
     4201 cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg
     4261 accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt
     4321 tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca
     4381 acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta
     4441 cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt
     4501 gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct
     4561 tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa
     4621 ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt
     4681 aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac
     4741 atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag
     4801 atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt
     4861 tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac
     4921 aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt
     4981 gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg
     5041 aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact
     5101 ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga
     5161 tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac
     5221 actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg
     5281 tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac
     5341 aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc
     5401 gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct
     5461 aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg
     5521 cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta
     5581 cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc
     5641 tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag
     5701 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     5761 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     5821 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     5881 tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag attagggtag
     5941 tatatgctat cctaatttat atctgggtag catatactac ccaaatatct ggatagcata
     6001 tgctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagcata
     6061 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     6121 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     6181 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     6241 tgctatcctc atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt
     6301 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     6361 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6421 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6481 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6541 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6601 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6661 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6721 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6781 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6841 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6901 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6961 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7021 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7081 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7141 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7201 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7261 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7321 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7381 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7441 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7501 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7561 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7621 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7681 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7741 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7801 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7861 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7921 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7981 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8041 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8101 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8161 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8221 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8281 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     8341 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     8401 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     8461 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     8521 gctctagcta gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca
     8581 acgttgttgc cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat
     8641 caatattggc aattagccat attagtcatt ggttatatag cataaatcaa tattggctat
     8701 tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca
     8761 atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     8821 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     8881 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     8941 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     9001 cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac
     9061 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg
     9121 cagtacatct acgtattagt catcgctatt accatg
//
