LOCUS       sequence-234389-        8634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_136690-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3412..3429
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3481..3536
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3535..3554)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5772..5790)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5858..5962
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5963..6031,6032..6823)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6181..6200)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6994..7582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7483..7502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7736..7753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7870..7891
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7906..7911,7912..7929,7930..7936)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7944..7960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7949..7971
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7968..7984
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7968..7984
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        8255..8634
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccaacaccc tgaagcccat cgagatccct
      841 ctgacccacg gcggcagcgt gatcggcaaa gaggaaatcg tcaacatcga cggccaggaa
      901 gtgaaagtca acagcttcct gggcatcccc tacgccagca agcccatcgg caagctgaga
      961 ttcgcccctc ccgagaagca ccccggctgg aagggcaagt acaacgccac caccctgtcc
     1021 cccacctgtt ggcagtacat cttcaccggc ttcgacgccg tgaacgccgc tggcaagatg
     1081 tggatcaaca acaccgagat gagcgaggac tgcctgtacc tgaacgtgtg gacccccaag
     1141 agcagcgtgg acagccccca tctgcccgtg atggtctgga tctacggcgg aggcttcacc
     1201 agcggcagcg ccaacctgca ggtctacaac ggcgccatcc tgagcgccac ccagaacgtg
     1261 atcatcgtgt ccatgcagta cagagtgggc gccttcggct tcctgcggct gaagcccaac
     1321 atcaccgacc agacccagac cgacgccctg ggcaaccagg gcctgctgga tcagctgatg
     1381 gccctgaaat gggtgtccga gaacatcggc cagttccacg gcgaccccaa ccaggtcaca
     1441 atcttcggcg agagcgctgg cgccgtgtcc gtgtccatcc tgtggatgag ccctatcgcc
     1501 cagccctact tcagacgggc catcctgcag agcggcagcc tgtacgccag atggggcctg
     1561 gacaacgccg acgaggccca cgagaaggcc gacgtgttca ccagagagtg cggctgccag
     1621 agccccagcg tggaccggaa ggccagcctg gaatgcctgc ggaagctgga ccccctgacc
     1681 ctggtcaacc agctggacag catcaacgtg gccatcggaa agcacagata cgacgccgtg
     1741 cggaagtacc tgctgcccag ataccacaag caggaaccct ttctgctgag ccagagcacc
     1801 agcacccggc tgtacttcga cgtgcccctg cagcccgtga tcgacggcta cctggtgccc
     1861 aagcaccccg accacatctt caacgagaag aacaagctga agcagaaccc cgagctgctg
     1921 atcggcgtga acaccaacga ggccatgttc tttctgctgc ccggaatcgc tatcaaggat
     1981 acccagttcc tgttcagcaa cggctccgtg atcatgccca gcaccatgga actggctggc
     2041 aagaagaagc ccttcaaaga gggcgaggaa atcgccgact tctactggat caccgccaca
     2101 cagatcctgg acgagagcca catgcggcct ggcctggcca agatgcccag ctactactac
     2161 aacctgcccc tgaccagcag ccccaagcgg ggctactacg accccgacac cgtgtacatc
     2221 cacgacgagg aactgctgcg gcggctggac aagttcgccg gcgacctgga tttcgcctgc
     2281 cccaccctga acttcgccga acaggtggcc cggctgccca acgccaaggt gttcctgtac
     2341 cacttcaaca agcggaccga gagcctgccc atgcccaagt ggacaggcgt gatgcacggc
     2401 tacgagatcg agtatatctt cggcatccct tacgaccctg agttcagcaa gcagttctac
     2461 aacttcaccg accccgagaa gatcttcagc agccggatca tgaagatgtg gaccaatttc
     2521 gccaagaccg gccaccccag caagagcaac gacggcaaga tcagcacccc cgagtggccc
     2581 ctgttccaca gcaccgacgg cttcgtgtcc aacaaccccg actacctgat cctggaagat
     2641 gagacaaagc tgggcagcgg cctgcaccgg gacagatgcg ccttctggct gcacgagatg
     2701 caggacatga aggacatctg gttcaacaga tgcgacccca gcggcggcat caagcccaca
     2761 ggcgcgccgt cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc
     2821 ttcccactca accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag
     2881 gctccttctt cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag
     2941 aagtctacta gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata
     3001 ccccaggtct cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg
     3061 atactgtatc aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact
     3121 ttgacctgtg aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag
     3181 aatcaggagg ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca
     3241 ggggtgtggc aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag
     3301 gttttatcca aagggttgaa ttccggcagc ctgcaccaca tcctggacgc ccagaagatg
     3361 ctgtggaacc acagggacag gaacctgcct cctctggccc ctctgggccc ccaccaccat
     3421 caccaccatt gagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct
     3481 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     3541 gaaggacata tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag
     3601 gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg
     3661 gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca
     3721 catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta
     3781 taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag
     3841 tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc
     3901 aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg
     3961 tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata
     4021 gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt
     4081 atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc
     4141 tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc
     4201 tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca
     4261 caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct
     4321 tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta
     4381 tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg
     4441 gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata
     4501 aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg
     4561 ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat
     4621 aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca
     4681 ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc
     4741 agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg
     4801 gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg
     4861 cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg
     4921 gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg
     4981 caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc
     5041 gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc
     5101 tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact
     5161 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc
     5221 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     5281 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     5341 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
     5401 taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc
     5461 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5521 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5581 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     5641 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5701 ctgtatccgg gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg
     5761 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     5821 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5881 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5941 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6001 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6061 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6121 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6181 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     6241 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6301 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     6361 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6421 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     6481 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     6541 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     6601 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6661 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6721 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6781 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6841 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6901 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6961 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7021 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7081 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7141 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7201 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7261 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7321 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7381 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7441 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7501 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7561 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7621 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7681 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7741 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     7801 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     7861 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     7921 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     7981 tgaccatgat tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta
     8041 tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg
     8101 aagatctata cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca
     8161 taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt
     8221 tatattggct catgtccaat atgaccgcca tgttgacatt gattattgac tagttattaa
     8281 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     8341 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     8401 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     8461 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc
     8521 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     8581 cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catg
//
