LOCUS       sequence-234390-        8100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_061970-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3258..3275
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3327..3382
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3381..3400)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5618..5636)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5704..5808
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5809..5877,5878..6669)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6027..6046)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6840..7428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7329..7348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7582..7599
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7716..7737
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7752..7757,7758..7775,7776..7782)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7790..7806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7795..7817
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7814..7830
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7814..7830
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccttcggcc
     1201 tgttcatctt catgagcggc ctgatgccca acgaccccgc caccaacgtg gaaaagatcg
     1261 gctacgtgga agagacatac aagatcgaca agttcgtgat gatcgtggtg gacgccctgc
     1321 gggccgactt cctgttcagc cccaagtaca gcgagaactg ggtcaagctg cggagctaca
     1381 tgaacctgca gagcgccacc tgtagcgcca gcatcgtgca gccccctacc gtgaccctgc
     1441 cccggatcaa ggccatcgtg tccggccgga accccaagtt cgtggacatc ctgcacaacg
     1501 tgaacgccga gacaatggtg gacgacaact gggtcacccg gctggtggac caccagtgga
     1561 acatccagtt ctacggcgac gacacctgga tcaagctgtt ccccaagagc ttccaggtgt
     1621 acgagggcac caacagcttc tacgtgaacg acttctacga ggtggacgcc aacgtgaccc
     1681 ggcacatcag caccctgatg accacccccg acaagtggga ctgcctgatc ctgcactacc
     1741 tgggcctgga ccacatcggc cacatcgagg gccctagcgg cagcagcatc cccaagaaaa
     1801 tccgcgagat ggacgaagtg atccaccgga tcctggaagt gctgatgaac agcagctcca
     1861 tcgtgaacaa gaactggctg ttcatcctga ccggcgacca cggcatcagc gacaagggca
     1921 gccacggcgg cagcaccacc ggcgagaaaa ccaccggcct gtttatgttc ggcagcaact
     1981 ggaacaagac caacgaccac ctgaccgaga atacctgcgt gaccaccaac ctgaagcagc
     2041 tgccccagac ccagcaggtc gacctcgcca ccctgatcgg cctgatcacc ggcgtgggaa
     2101 tccctgtgcg gagcctgggc atcctgagcc ccagctggct gcacaacttc tggtccacca
     2161 accacgaccg gctgaaggtg ctgtacaccc tgatccagca cgtgggccgg ctgagcggcg
     2221 agtgcgcctt cagcctgaag tccaaccacg aactcgagta caagaactgc atcagcaacg
     2281 tgtggtccga ggaacggacc accaagtaca acatcctgct gaacgagatc atcacctggc
     2341 tgcactgcca gaagatccag ggcaactcca ccaccgagtt caagcagatc gactgcgtga
     2401 agtccggcag cagactgaag ggcgaggaac tgatcaccaa caccgtgaac ctgctgaatg
     2461 gcatccagag cgagagcctg agcaaggtgg aacacctgag caacaagcag atgatcttcg
     2521 gcggcatcct gatgtggatc aacaagaagt ctctgatcca gtactactac agcgacaccg
     2581 ccgacctgag ctacagcttc gaccctggcg cgccgtcgac ctccatcacg gcctataaga
     2641 gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa agcctgcagg
     2701 gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc accttctccc
     2761 taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc cagctgtccg
     2821 aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct ggttctggca
     2881 acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg gtggtgatga
     2941 aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc acctcaccca
     3001 agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg caggagaaag
     3061 tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt gaaggtgaag
     3121 aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc ggcagcctgc
     3181 accacatcct ggacgcccag aagatgctgt ggaaccacag ggacaggaac ctgcctcctc
     3241 tggcccctct gggcccccac caccatcacc accattgagt ctagacacgt gattaattaa
     3301 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     3361 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     3421 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     3481 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     3541 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     3601 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     3661 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     3721 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     3781 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     3841 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     3901 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     3961 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     4021 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     4081 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     4141 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     4201 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     4261 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     4321 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     4381 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     4441 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     4501 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     4561 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     4621 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     4681 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     4741 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     4801 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     4861 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     4921 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     4981 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     5041 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     5101 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     5161 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5221 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     5281 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     5341 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     5401 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     5461 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     5521 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
     5581 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     5641 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     5701 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5761 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     5821 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     5881 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     5941 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6001 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     6061 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6121 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6181 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6241 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6301 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     6361 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     6421 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6481 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     6541 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     6601 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     6661 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     6721 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6781 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6841 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6901 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6961 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7021 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7081 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7141 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7201 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7261 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7321 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7381 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7441 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7501 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     7561 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     7621 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     7681 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     7741 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     7801 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
     7861 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     7921 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     7981 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     8041 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
//
