LOCUS       sequence-234392-        7113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_072190-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1891..1908
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1960..2015
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2014..2033)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4251..4269)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4337..4441
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4442..4510,4511..5302)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4660..4679)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5473..6061
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5962..5981
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6215..6232
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6349..6370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6385..6390,6391..6408,6409..6415)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6423..6439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6428..6450
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6447..6463
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6447..6463
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6734..7113
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccgtgcggt gttacgtgtg cgattactgc
      841 cccatcgtga ccagcgtgtc catcagcgag gaaaacaact gcaccagctg cagcaccgcc
      901 ggctacaact acagcatcca ccggatctgc gtgttcaagg acggcatccc catcaacttc
      961 cccaacgaga accggaccca gtgcaacacc gacctgtgca acggcctgac cgtggacaac
     1021 accggcaaga tccccagcgt gccaatcgcc aaccccttcc ggtgctacac ctgtctgaat
     1081 tgcaccaaga gcaaccagaa ggtgctgagc ggctgcggcg cctgtgtgac aacaagaggc
     1141 agcggcatca tcagcaagtt ctgcggcacc acatgcgagc ggctgtacat cgacgaccag
     1201 atcagctgct gctccaccga tctgtgtaat ggcatgacag gcgcgccgtc gacctccatc
     1261 acggcctata agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag
     1321 gaaagcctgc agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg
     1381 atcaccttct ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag
     1441 ttccagctgt ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt
     1501 gctggttctg gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac
     1561 ctggtggtga tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga
     1621 cccacctcac ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc
     1681 aggcaggaga aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg
     1741 agtgaaggtg aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat
     1801 tccggcagcc tgcaccacat cctggacgcc cagaagatgc tgtggaacca cagggacagg
     1861 aacctgcctc ctctggcccc tctgggcccc caccaccatc accaccattg agtctagaca
     1921 cgtgattaat taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca
     1981 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     2041 atcatttggt cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg
     2101 aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt
     2161 cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc
     2221 aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc
     2281 caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca
     2341 ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct
     2401 cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta
     2461 gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt
     2521 taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc
     2581 taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa
     2641 agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg
     2701 gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc
     2761 aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag
     2821 ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag
     2881 gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct
     2941 atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa
     3001 cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga
     3061 tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac
     3121 tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact
     3181 ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct
     3241 ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag
     3301 tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc
     3361 ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac
     3421 cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat
     3481 aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac
     3541 acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga
     3601 gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat
     3661 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     3721 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     3781 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     3841 atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat
     3901 atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc
     3961 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc
     4021 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     4081 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     4141 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     4201 tatcctcatg ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat
     4261 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4321 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4381 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4441 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4501 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4561 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4621 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4681 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4741 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4801 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4861 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4921 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4981 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5041 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5101 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5161 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5221 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5281 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5341 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5401 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5461 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5521 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5581 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5641 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5701 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5761 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5821 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5881 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5941 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6001 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6061 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6121 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6181 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6241 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     6301 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     6361 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     6421 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     6481 ctagctagag gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg
     6541 ttgttgccat tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa
     6601 tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg
     6661 ccattgcata cgttgtatct atatcataat atgtacattt atattggctc atgtccaata
     6721 tgaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     6781 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     6841 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     6901 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     6961 ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt
     7021 aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag
     7081 tacatctacg tattagtcat cgctattacc atg
//
