LOCUS       sequence-234394-        7089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_064430-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2247..2264
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2316..2371
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2370..2389)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4607..4625)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4693..4797
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4798..4866,4867..5658)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5016..5035)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5829..6417
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6318..6337
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6571..6588
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6705..6726
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6741..6746,6747..6764,6765..6771)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6779..6795
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6784..6806
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6803..6819
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6803..6819
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccgctagac
     1201 ctctgacacc ttaccagaga agcttcaccc ccgccaagaa catcagctgc gcccagtgcg
     1261 agatgaagct ggaagtggac aagagcaaga gcttcgtgga aaccgacatg taccggcgga
     1321 gatgcctgga cgagcccgag ctgttctaca agccctgcat cgtgaacgcc aagaataccc
     1381 ccagaggctg cgccaagctg accacctacc tgaaagaacc cgtggccaag ggcagagtga
     1441 aagaagtgac cctgatgaag cggttctgcg ccacagaggg cgccgagccc gaagagatca
     1501 gatgcgtgaa cagagccagc gagggcggct acagcgagct gtgtatctgc ggcaccgaca
     1561 actgcaacag cgcccctcag ctgctgaagt cctctggcgc gccgtcgacc tccatcacgg
     1621 cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt ggagaggaaa
     1681 gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag tcctggatca
     1741 ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac cccaagttcc
     1801 agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt cagtttgctg
     1861 gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa gtgaacctgg
     1921 tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg atgggaccca
     1981 cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg gtctccaggc
     2041 aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt ctactgagtg
     2101 aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg ttgaattccg
     2161 gcagcctgca ccacatcctg gacgcccaga agatgctgtg gaaccacagg gacaggaacc
     2221 tgcctcctct ggcccctctg ggcccccacc accatcacca ccattgagtc tagacacgtg
     2281 attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta ttttcattgc
     2341 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     2401 tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc cggacgaact
     2461 aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt
     2521 tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac
     2581 acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac
     2641 actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc
     2701 ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca
     2761 ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta
     2821 ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac
     2881 cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat
     2941 tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg
     3001 tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt
     3061 caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg
     3121 agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa
     3181 tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt
     3241 caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga
     3301 caccaatata accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt
     3361 ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc
     3421 catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg
     3481 gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat
     3541 ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa
     3601 cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc
     3661 cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc
     3721 ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact
     3781 gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt
     3841 aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac
     3901 ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc
     3961 acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc
     4021 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     4081 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     4141 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     4201 tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc
     4261 tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc
     4321 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc
     4381 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     4441 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     4501 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
     4561 ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct cgtgatacgc
     4621 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     4681 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4741 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4801 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4861 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4921 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4981 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5041 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5101 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5161 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5221 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5281 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5341 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5401 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5461 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5521 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5581 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5641 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5701 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5761 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5821 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5881 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5941 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6001 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6061 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6121 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6181 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6241 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6301 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6361 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6421 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6481 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     6541 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     6601 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     6661 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     6721 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     6781 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag
     6841 ctagaggtcg accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt
     6901 tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt
     6961 ggcaattagc catattagtc attggttata tagcataaat caatattggc tattggccat
     7021 tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac
     7081 cgccatgtt
//
