LOCUS       sequence-234401-        8568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_141680-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3346..3363
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3415..3470
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3469..3488)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5706..5724)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5792..5896
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5897..5965,5966..6757)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6115..6134)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6928..7516
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7417..7436
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7670..7687
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7804..7825
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7840..7845,7846..7863,7864..7870)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7878..7894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7883..7905
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7902..7918
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7902..7918
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        8189..8568
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccatcaacg acaacaacct gtggcaggtc
      841 atcaccagct accccaacgc caccaagttc gccgacttca tccaggaaat cggcctgcag
      901 agcagcctgg aaaagcccgg ctgcctgggc gacgacgagt gcctgaccat catcctgccc
      961 accaacgacg ccatcgacct gatggccaac gacctggcct ggtactccct gggcgactac
     1021 cagaagcaca gcttcatcca cggccacatc atccagcggg gcatcaccac cggccagagc
     1081 agcgtcagag tgaccaccag agagtggacc aagctgggcc agcagatgaa cttcatcgac
     1141 atcggcttcg gcaccggcgt gaagtacacc accctgttcg ccaagcagag attcggcttc
     1201 agcacaatcg ccgacagcac ccccctgtac ttcgtgaaca acgccaagat catgaccccc
     1261 gacatcatgg ccagcaacgg catcatccag atcgtgaacc gggtgctgta caccccccac
     1321 atcagcctgc ccttcatgga atttctgcag aagcagggca acctgggcat cagcaaagag
     1381 ctgtggtatc tgctgctgca ggaacctaag tacgccctgt accccgccca gcagatctac
     1441 agcaccatct tcgtggtgtc cgacagcggc tggaacagcc tgcccatcac ccagctgaac
     1501 cggctgcgga agaacatcac cctgctggcc agcgtgctga gctaccacta ctgccccaat
     1561 cagctgatct accgcgagtg gatcagcgtg aagcccaaca tgaacatcta caccggcttc
     1621 cccaaccggc cccagagcgc cagctctatc cagcccagcg agctgaacag cgcccagctg
     1681 tacaagagca gcggcgacag catcatcatc agcagcggca acaccgaggc caagctggaa
     1741 gataagagct tcatcattca gaaggccatc gtccacatta tccagaagcc cctggccttc
     1801 atcttcgaga cacgggaaga gatcctgaac cggatcaaca gcaacctgct gtacagctgc
     1861 cagctggaca ccatctgcaa gaacatcctg accagcagca ccgacatcac catcttcagc
     1921 cccaaccccc aggccatgca gaacttcaac aagctgagca ccaacgagaa agccatctac
     1981 ctggaatacc tgttcctgcc catgaagctg taccaggccg agatgaccct gagccggcgg
     2041 atccccgtgg acaagctcga ggacgccatc cggttcaaga tcatcgacca gagcaccttc
     2101 atcgaggcca gactgcccaa ccagggctac gtggccgcca gaatcatcaa cgccaaccag
     2161 gtggccacca atggcatcgt gcacatcgtg gatgccatcc ccggcctgcc ctacgagaca
     2221 gtgcagcagt acgtggcccg gatcagcgac ttcaccctgt actacaatac cggcttcatc
     2281 cagagcatga ccgtgggcga gccctacacc ttcctggtgc ccaccaatca ggccatgacc
     2341 agcatgaaca acgacatcga agtgggcaac aagctgctga ccgacaagct gcggaaggac
     2401 ttcgtgttca gacggaacac cttccccctg gacctgatca tccaggacct ggacatccgg
     2461 aaggtgtact acgtgtccac cgccaactac gccttcacca gagagcagct gcggctggaa
     2521 atcaactcca agaacagcga caagggcgtg ttcacctacc gggacgagcg gatccaggtg
     2581 ctgagcctga acggccccta ccagttcacc aacggctttc tgtacagcat cgacaagatc
     2641 ctgtacacca agagcgacct gaacgccacc atgtgcaccg tgtccgcctg cgctggcgcg
     2701 ccgtcgacct ccatcacggc ctataagagt gagggggagt cagcggagtt ctccttccca
     2761 ctcaaccttg gagaggaaag cctgcaggga gagttgagat ggaaggcaga gaaggctcct
     2821 tcttcccagt cctggatcac cttctcccta aagaaccaaa aggtgtctgt gcagaagtct
     2881 actagcaacc ccaagttcca gctgtccgaa acgctcccac tcacccttca gataccccag
     2941 gtctcccttc agtttgctgg ttctggcaac ctgaccctga ctctggacag agggatactg
     3001 tatcaggaag tgaacctggt ggtgatgaaa gtgactcagc ccgacagcaa cactttgacc
     3061 tgtgaggtga tgggacccac ctcacccaag atgagactga tcttgaagca ggagaatcag
     3121 gaggccaggg tctccaggca ggagaaagtg attcaagtgc aggcccctga agcaggggtg
     3181 tggcaatgtc tactgagtga aggtgaagag gtcaagatgg actccaagat ccaggtttta
     3241 tccaaagggt tgaattccgg cagcctgcac cacatcctgg acgcccagaa gatgctgtgg
     3301 aaccacaggg acaggaacct gcctcctctg gcccctctgg gcccccacca ccatcaccac
     3361 cattgagtct agacacgtga ttaattaagg atcccccgac ctcgacctct ggctaataaa
     3421 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     3481 catatgggag ggcaaatcat ttggtcgaga tccctcggag atctctagct agaggatcga
     3541 tccccgcccc ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag
     3601 tgcatgtaat cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg
     3661 acacgggggg ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg
     3721 gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg
     3781 gactgcaaca caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct
     3841 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
     3901 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
     3961 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
     4021 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
     4081 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
     4141 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
     4201 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
     4261 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
     4321 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
     4381 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
     4441 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
     4501 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
     4561 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
     4621 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
     4681 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
     4741 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     4801 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     4861 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     4921 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     4981 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     5041 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     5101 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     5161 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     5221 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     5281 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     5341 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     5401 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     5461 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     5521 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     5581 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     5641 ccgggtagca tatgctatcc tcatgataag ctgtcaaaca tgagaatttt cttgaagacg
     5701 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     5761 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5821 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5881 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5941 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6001 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6061 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6121 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6181 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6241 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6301 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6361 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6421 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     6481 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6541 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6601 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6661 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6721 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6781 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6841 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6901 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6961 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7021 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7081 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7141 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7201 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7261 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7321 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7381 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7441 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7501 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7561 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7621 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7681 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7741 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     7801 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     7861 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     7921 tgattacgcc aagctctagc tagaggtcga ccaattctca tgtttgacag cttatcatcg
     7981 cagatccggg caacgttgtt gccattgctg caggcgcaga actggtaggt atggaagatc
     8041 tatacattga atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc
     8101 aatattggct attggccatt gcatacgttg tatctatatc ataatatgta catttatatt
     8161 ggctcatgtc caatatgacc gccatgttga cattgattat tgactagtta ttaatagtaa
     8221 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     8281 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     8341 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     8401 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
     8461 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
     8521 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatg
//
