LOCUS       sequence-234403-        9393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 9393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_170550-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..9393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4171..4188
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4240..4295
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4294..4313)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6531..6549)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6617..6721
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6722..6790,6791..7582)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6940..6959)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7753..8341
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8242..8261
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8495..8512
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8629..8650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8665..8670,8671..8688,8689..8695)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8703..8719
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8708..8730
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8727..8743
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8727..8743
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        9014..9393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccaatctgt gccccagaca gtgcctgcct
      841 ggcagctgct gcgaggtgga agagaacgac ttccggtgct gcccctacgc caacggcgtg
      901 tgctgcagcg acggcaagaa ctgctgtcca cagggcacca agtgcgacat ggaactgggc
      961 cagtgccggt cctccaacgg cgccgtgctg ctgagcaagc acatgagcga gaaagtgatc
     1021 ggcgacctga aaaccagcgc cctggacgtg gtgctgcggc ggctgagcgc caactacatc
     1081 atgtgccccg accggctgag cgtgtgccag gacaacacca cctgttgccc catgagcggc
     1141 gagatctggg gctgctgcca gcacgccaac gccatctgct gccccgatgg cgagcactgc
     1201 tgtccccacg gcagcgtgtg cgatatggtc aaccacagct gcaagcccgc caagagcaac
     1261 gagatccccg agatgaagga aagcttcccc accctgcacc tggtgtccgc ctctgcccct
     1321 gccagactga accccaacgt gaagcccagc aaggccagcc ggcggaagaa catccaccag
     1381 cccagcgcca agtacctgct ggcctacagc ggcgtcgtgt gccccgatcc cctgtacgag
     1441 tgccccgaca acacaacctg ctgcagctcc agcgacgaga agtgggcctg ctgccccatc
     1501 ccccagaccg tgtccttcct gggcgtgtcc gacaaggccc tgcacaagag cctggaacag
     1561 ctgcacagca acaagaacag cagctacagc gtggccaact tcaacctgac ctgggtccga
     1621 ctgagcatcc tgaacggcac ccagctgtgc cctgacgcca agagccggtg cagcgacgag
     1681 cagacctgtt gcaagctgaa aaccggcgag tggggctgtt gccccgtgaa gaacgccgtg
     1741 tgctgttccg acggggagca ctgttgccct aacggctaca cctgtgacct gagcgtgggc
     1801 gagtgcatca agaacagcca cagcagcaac gagagcaaga agttcagcag caacaccatc
     1861 ctgagcagcc tgcccatcaa gatcggcagc aacgccctgt gtcccgacag cgacgtgtac
     1921 tgctccgaca acagcacatg ctgcgagcac gataagaaat ggggatgctg cctgttcccc
     1981 aatgccgtgt gttgctccga tggcatccac tgctgccccc agaacaccat ctgcgacgtg
     2041 caggaagaga tgtgcatcag caagagcggc gacatgatca ccaagctgca gctgaagtcc
     2101 acccccaaac tgcacagctt caagagcaac agccggatca acatctgcag cgacatgaag
     2161 tccgtgtgca tcaacggcac ctgttgcagc aacgtgaacg accccctgag caccctgtgc
     2221 tgcccttacg agaatgctgt gtgctgctct gacggcaaac actgttgtcc caagggaacc
     2281 acctgtgaca tgatcaacgg cggctgcatc atcagccagg aaggcctgag caagagcaac
     2341 tccatccccc tgctgaccaa gatccgggcc ctgcacgtga ccagcaagaa caacacccag
     2401 agcatcgtgt tcacccagat ctgccctggc ggcaaggcca agtgccccga tagctccact
     2461 tgctgcaagc tgagcagcgg gaagtacgcc tgctgtccta tccccaacgc catttgttgc
     2521 gccgacgata tccattgctg ccctcccggc accagctgcg actacaagac cctgagctgc
     2581 gtgaaggcca acaacgacca cagcatcggc agcccctccg ccaatgagct gctgcggaac
     2641 cccaagctgc tgttcaccgg cgtgcgggac cacgtgtgcc ctggccacca gagcacctgt
     2701 tccgatgacc agacctgctg ccggctgccc gacaagagct gggggtgctg ccctctgaag
     2761 aatggcgtgt gttgtggcga ccacttccat tgctgtccag ccggctccgt gtgcgacctg
     2821 gacaccaagc agtgcatcct gcagctggaa ctgaaccagt ccaccaccaa gatcgtgtac
     2881 gacggcttca ccatgcacat tgccaacaga aagagcagcg acagactgta cagccagccc
     2941 gtgaagtcca tcagcaccat cgacgccagc gtgcaggcca agtggtgcgg cgcctgtggc
     3001 gatacctggg cctgttgccc agacgccacc ttcaacagct ggtcctgctg cccatacgtg
     3061 ggcggcgagt gctgcatcgg ccagaacatc tgttgccccc ctggcagccg gtgcctgaac
     3121 aacggcaagt gcgagaagat ctccaagaac atcatgaagt ccttcgcccc caccctgatc
     3181 cccagcatct ctaagaacaa caacctgcag aagtcctact tcatccagca gaacaacgtg
     3241 aaagagaact tccacatcga gatgagcaat atcgccaagc ccgcccagcg gaacatcctg
     3301 ctgctcgtgc ggcactaccg gcggctgaag cggcagctgc acacaatctg ccccgacagc
     3361 ctgcactact gcggcaagag cgagcagtgc tgcctgtcca acaagttcgg ctacacatgc
     3421 acccccaaca agagcatctg ctgcggcagc aacatggaca cctactgccc catctacaag
     3481 gactgcagcc tggacggcaa gttctgcatc gataagtatg gcgcgccgtc gacctccatc
     3541 acggcctata agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag
     3601 gaaagcctgc agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg
     3661 atcaccttct ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag
     3721 ttccagctgt ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt
     3781 gctggttctg gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac
     3841 ctggtggtga tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga
     3901 cccacctcac ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc
     3961 aggcaggaga aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg
     4021 agtgaaggtg aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat
     4081 tccggcagcc tgcaccacat cctggacgcc cagaagatgc tgtggaacca cagggacagg
     4141 aacctgcctc ctctggcccc tctgggcccc caccaccatc accaccattg agtctagaca
     4201 cgtgattaat taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca
     4261 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     4321 atcatttggt cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg
     4381 aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt
     4441 cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc
     4501 aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc
     4561 caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca
     4621 ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct
     4681 cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta
     4741 gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt
     4801 taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc
     4861 taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa
     4921 agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg
     4981 gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc
     5041 aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag
     5101 ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag
     5161 gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct
     5221 atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa
     5281 cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga
     5341 tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac
     5401 tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact
     5461 ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct
     5521 ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag
     5581 tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc
     5641 ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac
     5701 cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat
     5761 aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac
     5821 acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga
     5881 gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat
     5941 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     6001 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     6061 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     6121 atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat
     6181 atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc
     6241 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc
     6301 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     6361 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     6421 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     6481 tatcctcatg ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat
     6541 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     6601 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6661 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6721 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6781 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6841 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6901 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6961 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7021 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7081 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7141 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7201 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7261 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7321 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7381 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7441 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7501 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7561 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7621 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7681 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7741 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7801 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7861 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7921 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7981 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8041 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8101 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8161 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8221 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8281 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8341 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8401 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8461 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8521 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8581 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8641 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8701 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     8761 ctagctagag gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg
     8821 ttgttgccat tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa
     8881 tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg
     8941 ccattgcata cgttgtatct atatcataat atgtacattt atattggctc atgtccaata
     9001 tgaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     9061 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     9121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     9181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     9241 ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt
     9301 aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag
     9361 tacatctacg tattagtcat cgctattacc atg
//
