LOCUS       sequence-234405-        7407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_128590-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     502..521
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      544..553
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1981..1998
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2050..2105
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2104..2123)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4341..4359)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4427..4531
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4532..4600,4601..5392)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4750..4769)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5563..6151
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6052..6071
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6439..6460
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6475..6480,6481..6498,6499..6505)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6513..6529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6518..6540
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6537..6553
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6537..6553
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6824..7203
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7204..7407
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7358..7378
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     join(7404..7407,1..21)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cgtttagtga accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg
       61 ttgggctcgc ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac
      121 ccgtcggcct ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg
      181 atcggaaaac ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac
      241 cgtggcgggc ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat
      301 gtaattaaag taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga
      361 gatccagctg ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat
      421 tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact
      481 tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag
      541 tttgccacca tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt
      601 gcggccgcca aagagaacca gggcgacaac gactggtcta tgatcatgga agtggaagtc
      661 gaggacccca cctacaacag cagcagcgac aagaccgacg gccggaactt catcatcaac
      721 ggcaagaaga accagccctt cctgcagaac aacaacgaga cagtgaccga gcacgccacc
      781 agccagtaca gcatcagcat ccctggcgct accatctgcg gcagctttct gtgctacaac
      841 cagggccagt gctacaccaa cctgcacagc agctccagca agtgcctgtg ccccatcggc
      901 tacagcggcg acttctgcga caaggtggac gagggctacc agttccccta catcgagcag
      961 ggcggctacc tgaagttcta cttcaagagc agcgccctgt acagcggcat caaccagagc
     1021 ttcatcgagc ggagcaacca ggtgtccatg aacagcacca acacctataa ggtggacctg
     1081 gaaatccggc ccagcctgac cgacggcagc tacctgctgg tgtcccacga gagcaacaag
     1141 ggcgacttca agttcagcct gagcatcgag aacggctacg tggtgtacag aagcctgcgg
     1201 atcagcaaga ccaactacga gccctaccgg aaagaggaaa tccaggaagt caagcacttc
     1261 acctttctga acaccctgaa gaacaacatc atcagccacc tgggcagacg gctgatggaa
     1321 aaagtgtctg gcgcgccgtc gacctccatc acggcctata agagtgaggg ggagtcagcg
     1381 gagttctcct tcccactcaa ccttggagag gaaagcctgc agggagagtt gagatggaag
     1441 gcagagaagg ctccttcttc ccagtcctgg atcaccttct ccctaaagaa ccaaaaggtg
     1501 tctgtgcaga agtctactag caaccccaag ttccagctgt ccgaaacgct cccactcacc
     1561 cttcagatac cccaggtctc ccttcagttt gctggttctg gcaacctgac cctgactctg
     1621 gacagaggga tactgtatca ggaagtgaac ctggtggtga tgaaagtgac tcagcccgac
     1681 agcaacactt tgacctgtga ggtgatggga cccacctcac ccaagatgag actgatcttg
     1741 aagcaggaga atcaggaggc cagggtctcc aggcaggaga aagtgattca agtgcaggcc
     1801 cctgaagcag gggtgtggca atgtctactg agtgaaggtg aagaggtcaa gatggactcc
     1861 aagatccagg ttttatccaa agggttgaat tccggcagcc tgcaccacat cctggacgcc
     1921 cagaagatgc tgtggaacca cagggacagg aacctgcctc ctctggcccc tctgggcccc
     1981 caccaccatc accaccattg agtctagaca cgtgattaat taaggatccc ccgacctcga
     2041 cctctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     2101 tctcactcgg aaggacatat gggagggcaa atcatttggt cgagatccct cggagatctc
     2161 tagctagagg atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc
     2221 ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc
     2281 cctgttccac atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg
     2341 acatccttat aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag
     2401 actttgcagt ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc
     2461 tcttacacca atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct
     2521 acaccaacgt caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca
     2581 ttagcaatag tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg
     2641 ggtagtagta tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga
     2701 agcatatgct atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac
     2761 cccatgagct gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca
     2821 ttttagtcac aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct
     2881 tcgcctgctt cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag
     2941 taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt
     3001 ggacgggggg ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct
     3061 tgggcaataa atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc
     3121 atggggtggg gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg
     3181 gcaacacata atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg
     3241 accaagacag gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac
     3301 gccgacagca gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc
     3361 acgccaatgg ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt
     3421 gggggcacgc gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt
     3481 aataacttgg ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac
     3541 cactaatggc accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc
     3601 gattgctgcg atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt
     3661 tgttggtcct catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta
     3721 gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat
     3781 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     3841 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     3901 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     3961 atgctatcct aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat
     4021 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc
     4081 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     4141 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     4201 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     4261 tatcctaatc tgtatccggg tagcatatgc tatcctcatg ataagctgtc aaacatgaga
     4321 attttcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4381 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4441 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4501 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4561 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4621 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4681 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4741 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     4801 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4861 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4921 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4981 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5041 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     5101 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5161 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5221 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5281 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5341 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5401 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5461 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5521 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5581 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5641 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5701 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5761 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5821 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5881 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5941 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6001 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6061 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6121 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6181 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6241 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6301 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     6361 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     6421 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     6481 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     6541 aaacagctat gaccatgatt acgccaagct ctagctagag gtcgaccaat tctcatgttt
     6601 gacagcttat catcgcagat ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg
     6661 taggtatgga agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt
     6721 tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat
     6781 atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg attattgact
     6841 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     6901 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     6961 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     7021 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     7081 agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     7141 atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     7201 atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga
     7261 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     7321 gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta
     7381 cggtgggagg tctatataag cagagct
//
