LOCUS       sequence-234408-        8271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_144130-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3049..3066
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3118..3173
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3172..3191)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5409..5427)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5495..5599
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5600..5668,5669..6460)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5818..5837)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6631..7219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7120..7139
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7373..7390
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7507..7528
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7543..7548,7549..7566,7567..7573)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7581..7597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7586..7608
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7605..7621
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7605..7621
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7892..8271
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccctggaca tccagaagga catctacctg
      841 aacgaccgga acatcagcgt gctgcaggtc aaccccttcg ccaaccccag cgactacatc
      901 accgagacaa tgaaggtcaa acggctgaac aagagcaagc tgagcaacag cctgaagtcc
      961 cagagcctgc acagacgggg cgacctgatc gagagcgacc agaacgacat catcaagatc
     1021 aaggccgtgc tgaacaacac cgccctgctg acctgcagac ccgacaagaa gctgctgacc
     1081 gagaacggca tcgacgagaa caccaagggc accctgctgt ggaagcggac caagaccaac
     1141 gagtacctga tccacaacaa ccggcggctg caccccgaca cccggtacat cctggacatg
     1201 agcagcttcg accagaccat cctggacctg cggatcgacc acgtgcagcg gaccgacgag
     1261 ggcgagtacg tgtgcctgta cagcaacggc cagaccgtgt acaagcagcg ggtgtacctg
     1321 aacgtgctgg tgccccccgt gatcagcgag aacagcagca gccccaccag agtgatcgcc
     1381 cacgaggggg aggccgtgat cctgcagtgt aaagcctggg gcgtgcccga gcccatcatc
     1441 acctggtatc tgacccccaa agagggccgg cagtaccgga tctacgaggc caccgacaag
     1501 agattcgtga tcaagcccaa ccagctgatc atcagcaaca tcacccggga cctgcagggc
     1561 acctacaagt gcctggccca gaacggcctg aaagagaaca cctggcgggt catcgagctg
     1621 gacgtgcgct acccccccac catcgagatg gccaacctga agctgggcca gctgctgaag
     1681 ggcaccacca tggtccgagc cgtgatcgcc ggcaacccca tcaacctgtt ctactgggag
     1741 ttcgagggca gacccatcca cggccccaac agcaactgcc tggtgcccat gcccaacgag
     1801 aagtactgca tcctggtgga caaggccaac agcaagcacg gcatcatccg gaccaccctg
     1861 tttatcgcca acctgaccat ggccaactac ggccactaca cctgtgtggt ggaaaccccc
     1921 ttcggcacct tccggaacag caccgagatc ttccggacct acagcagccc ctccaccacc
     1981 tgggacaaca gcttcggcgg caactacgac caccacttcc ccaacctgct gaccaactac
     2041 cagggcagct acgagaagat gagcgagaag ctgaacaagc gggccaccta cctgacccag
     2101 gaccggctga agcccagcag ctacagctcc ctgagcagcg tgaccggcca gaacgacttc
     2161 aacagcatct ccaccaccaa caccgtgaac cagcggcagc cccccatctg ccctacctgc
     2221 ctgaagtaca agaacgtgaa ggacctgacc accctgagcc ccgaccccat gaccctgaac
     2281 accttctccg actacaacta cctgcggaac ctgtccaaca ccctgaagct gagcctgagc
     2341 accttccacc tgatctggct gcacatcttc accagctttc tgcactactg cttcgtgggc
     2401 gcgccgtcga cctccatcac ggcctataag agtgaggggg agtcagcgga gttctccttc
     2461 ccactcaacc ttggagagga aagcctgcag ggagagttga gatggaaggc agagaaggct
     2521 ccttcttccc agtcctggat caccttctcc ctaaagaacc aaaaggtgtc tgtgcagaag
     2581 tctactagca accccaagtt ccagctgtcc gaaacgctcc cactcaccct tcagataccc
     2641 caggtctccc ttcagtttgc tggttctggc aacctgaccc tgactctgga cagagggata
     2701 ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc agcccgacag caacactttg
     2761 acctgtgagg tgatgggacc cacctcaccc aagatgagac tgatcttgaa gcaggagaat
     2821 caggaggcca gggtctccag gcaggagaaa gtgattcaag tgcaggcccc tgaagcaggg
     2881 gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga tggactccaa gatccaggtt
     2941 ttatccaaag ggttgaattc cggcagcctg caccacatcc tggacgccca gaagatgctg
     3001 tggaaccaca gggacaggaa cctgcctcct ctggcccctc tgggccccca ccaccatcac
     3061 caccattgag tctagacacg tgattaatta aggatccccc gacctcgacc tctggctaat
     3121 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     3181 ggacatatgg gagggcaaat catttggtcg agatccctcg gagatctcta gctagaggat
     3241 cgatccccgc cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg
     3301 cagtgcatgt aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat
     3361 gtgacacggg gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa
     3421 atggatgtgc acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct
     3481 gtggactgca acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat
     3541 gctgggggac atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca
     3601 atcagagggg cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg
     3661 tttataaggc ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata
     3721 tactatccag actaacccta attcaatagc atatgttacc caacgggaag catatgctat
     3781 cgaattaggg ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt
     3841 cacggtttta tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa
     3901 gggcagtggc tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct
     3961 tcattctcct tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt
     4021 gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt
     4081 cagtggtggc attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat
     4141 actagtgtag gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga
     4201 caagccgtaa agactggatg tccatctcac acgaatttat ggctatgggc aacacataat
     4261 cctagtgcaa tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt
     4321 gaaccatgtt gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc
     4381 ggactccact ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg
     4441 cccataaaca aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt
     4501 cagcccccac acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct
     4561 gattgtaacc ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac
     4621 cccggggaat acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat
     4681 ctggaggaca aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca
     4741 tattcacgag gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc
     4801 caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa
     4861 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     4921 tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     4981 tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa
     5041 tagagattag ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa
     5101 tatctggata gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     5161 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     5221 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     5281 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     5341 tatccgggta gcatatgcta tcctcatgat aagctgtcaa acatgagaat tttcttgaag
     5401 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     5461 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5521 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5581 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5641 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5701 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5761 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5821 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     5881 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5941 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6001 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6061 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6121 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6181 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6241 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6301 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6361 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6421 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6481 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6541 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6601 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6661 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6721 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6781 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6841 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6901 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6961 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7021 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7081 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7141 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7201 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7261 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7321 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7381 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     7441 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     7501 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     7561 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     7621 ccatgattac gccaagctct agctagaggt cgaccaattc tcatgtttga cagcttatca
     7681 tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag
     7741 atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa
     7801 atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat
     7861 attggctcat gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag
     7921 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     7981 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     8041 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     8101 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct
     8161 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg
     8221 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat g
//
