LOCUS       sequence-234410-       13065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13065)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 13065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_154760-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..13065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(6695..6706)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             8229..8246
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    8298..8353
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8352..8371)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10589..10607)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10675..10779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10780..10848,10849..11640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10998..11017)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11811..12399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12300..12319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12553..12570
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12687..12708
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12723..12728,12729..12746,12747..12753)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12761..12777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12766..12788
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12785..12801
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12785..12801
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 agctgaaccc ctacttccag ttcatccccc agaacgacaa cctgcacacc aagatctacg
     1261 actacagcga gggcttcgac gaggacagct tcacctacga gctgcagtac cccatcccca
     1321 tctacgagac actgatcaag aagatcacca tccggaagaa cggactgctg gccctggaca
     1381 gcatcagcgg cgacaatctg cccagcgcct accccgtgaa cacatccatc accaaccagc
     1441 ggctgcagga cttcgacggc agatacctga gcatcttcag cagcgtgaac gacggcaaga
     1501 gcggccggat cagagtgacc gagatcgacc tgctgagccc cagcaagctg ccctgcgtgg
     1561 aacggatgca gatcgacaga ctgatcaagt tcatccacct gagctacatc gaggaagccg
     1621 agctgaaggc ccagtacatc gtgcacatcc tgtgggagaa catgaccaac gcctacctga
     1681 tcaaccagaa ggccaacacc tacggcatga ccatcatcag caacggcatc cggtcctacg
     1741 ccttcatgca gtacatccag gtggaatgga acgagaacga cgtggccgag aacctggccc
     1801 tgccccccga gagcggcatc ttcatgaggc ctctcggcgg ctaccagctg cccagatcca
     1861 gccaggccgt ggaacccaac atctggctga ccgagacaaa tatcgccctg cacggcgagt
     1921 ggctggtgcc cctgtacaag agcgagaata tcgccaccat caacgtgaag aacctgagcg
     1981 ccgtggccag cgagttcgtg gctagcctgg ccagcatctg caagggcagc ctgcacagcc
     2041 tgaagtccaa caacgagaca cccagcgaca ccgtggtcat caagaacgtg aacaccgaga
     2101 acaccagcga ggaacctatc gcccggaccc acagcttcaa gcgggccctg gaaaatctgg
     2161 aaaactacga cctgaacgtg tccaccaacg actactccaa cggcatcacc gagaacgtgc
     2221 ccaaggccgc ctggaacatc ctgcgggacg aggccgagat ctccggcgac gaccccttca
     2281 accagaacga agtgaatatc ctgcccgaga acgagacagg caacagcttc taccacgaga
     2341 gcaacaagtt cgtggtggac aacaaagagc acacctacac cgacaccatc accgcccaga
     2401 cctactaccc cctgggcaga tacgacggcg acgaggaacg gcaggaaatc cacgagcccc
     2461 tgatcgtgcc cgtgaccatc caccccctga gcgacgacga cgatgacgag gtgctgatcg
     2521 gcaacaacgg cgagtacaag agcatgaacg agacattcag caacgacctg gtcaagtgcg
     2581 acgccgtgtc cgagtgcaac agccacaaca ccgagtgcta ccgggtggac aacgccgcct
     2641 gctgtatctg cagcgacggc ttcttcggag ccggcaacgc caagtgttgg agcgaagtga
     2701 acgaccacaa gttcagcttc agcggcagcc tgaccgccag attcggcagc gagcacagca
     2761 gcagccccct gcacatctac ctggacatcc agcacggcag cctgcggcgg agcagctctg
     2821 gaatcagagg cggccagacc aacgaccgga tctacagcac catccggctg atcacccccg
     2881 tgttccacat cctgaacagc atgatcagca gcccctgcga gaacatcccc aagcgcgagc
     2941 gggtgtacaa cctgttcacc ctgggcaacg gcctgcagcg gcccttcaag gtgctgttcc
     3001 agttcgacat cgagagagtg ggcaagttca caatccaggc catcctggaa ctgcgggact
     3061 tcaccaccgg cgcctacgcc agcggcgtga tcgagctgga agccttcagc accgagaatc
     3121 tgaacctgct ggaccggtct tacatcgagg cctacagcgc cagcgacaag cccgacaaga
     3181 cctactatgc caactacaag atcgacgacc acggccggat cgtgttcccc gagcagaccg
     3241 tgaagttcat cgtggaacac cgggacagca acgagatcag caacttcccc gaagagatca
     3301 atgtgcattg gagcgctgtg gccgagagcg agagctgcct gctgaaacag gtgtccagca
     3361 tccccaagaa tgagtggtac tacatcagac tgaaggaccg gggctactgc agcgaggact
     3421 gtagcgcccc tgacggcagc tgcaacctgt tctgcgtgga cgagcctctg ctgctggcca
     3481 ccgagcccag cccttgcgac tgcgtgacct gcgaccggca cggcgaagtg tgcagacccg
     3541 agggcgccag ctacagatgc gagtgcggcc agggcctgaa gctgatgcct gacaatacct
     3601 gccaggccac aagcctggtg gacgtggcca actacctggg cgtgcagtgc ggctccgtga
     3661 actgccacac ccacgctcgg tgcatcgacc ccaaccaggc cttctgccag tgcctgcccg
     3721 gctacagagg cgatggcgtg tcccactgcg agaacgaccc ctgcagcaag tgccggcgga
     3781 acgagatctg cgagaatggc atctgtatcg cctccggcgt ggacctgtgc gagggggtcc
     3841 agtgtggcga gcaggccttt tgtcaggacg gcgcctgcgt gtgcaccccc ggctatacag
     3901 gcgaccccgt cgtgaagtgc tacgaggaaa gggacccatg cgccaacgtg cggtgccacc
     3961 cctacgccaa gtgccggaac ggcaactgcg agtgcccccc tgactacgat ggcgacggct
     4021 accaagtgtg caagccccgg aactacgacc cttgcagcca tgtgcagtgt cactacgacg
     4081 ccacctgtta caacggcgtg tgtacctgca gaaacggcta catcggcgat ggatacagag
     4141 agtgccggcc cagagaaacc gacctgtgcc accacatcca gtgtcaccct tacgcccagt
     4201 gcaagaatgg ccagtgccat tgcatcgagg gctatatcgg ggatggctac tacgactgcc
     4261 acgtgcggga tccttgcacc ggcgtgaagt gccatcacta cggcgagtgc atctctggca
     4321 gatgccactg cctgagaggc tatgaggggg atggctacta tgattgccgg gaaaccgtgt
     4381 ccgacccctg tagcagcgtc cgctgtcacc ccaacggcta ctgcaagaac ggccggtgtc
     4441 tgtgtctgaa cggctatgaa ggggacgggt actacgagtg cagacccttc tacaacgatc
     4501 cctgcgtggg agtgcagtgc cacagcaagg ccgagtgtcg gaatggctac tgccggtgcc
     4561 tgaatggata cgaaggggac ggcttccact actgctacgc catgcaggat gacccctgct
     4621 ccagcatgag atgccacatc aatgccaagt gcttcgatgg cgagtgcaga tgcctggatg
     4681 gctaccaggg cgacggaaag cactactgcg accccatccc tcaggaccag tgccagaaca
     4741 tccggtgcca cgagttcgcc aactgccaga acggaagatg ccagtgcaac gccggatatg
     4801 agggggacgg atattgggag tgcaagagca tcaagagcga cctgtgcaaa tacgtgcgct
     4861 gccacgagaa cgccaaatgc aacgagtacg gcaagtgcca gtgtctggac ggatactccg
     4921 gggatggata ttacgagtgc cagaagtcca gccaggaact gtgcgctggc gtccagtgcc
     4981 atagattcgg ccagtgttac gagaaccggt gctactgctc ccacggctac gtgggggatg
     5041 gcgtgaactt ctgcgacgcc agggccaacg acccatgcga cggcgtcaga tgcgccgcca
     5101 atggacggtg ccaggacggc agatgcgtgt gcgatcccgg atataccggg gacgggtaca
     5161 atgagtgtcg cgaggccgag ggcgtgaacc tgtgtggcaa cgtccagtgt catcagtacg
     5221 ccacatgcga ccggggccag tgcagatgtg tgaccggcta tgacggggat gggtatagcg
     5281 actgcaggcc cgtgaccgag gacaagtgca gcagagtgcg gtgtcatccc gatgcccagt
     5341 gcaccgacgg ctactgtttc tgccccagcg gctttgaggg cgacggctat tatgaatgca
     5401 agcggatcac ccaggacaga tgtgccaatg tccggtgtca cgagaatgct aagtgcgacg
     5461 acggatactg cagatgcaaa gaggacttcg agggggacgg ttactctgag tgcaggcgga
     5521 agtccgagga cccttgcgcc cggatcagat gccatccaca ggcccagtgc gagtacggct
     5581 tctgccggtg taaaaacggg tacaaggggg atggttattg gaactgccag cccatccagt
     5641 ccgacctgtg tcgggccgag cagtgccacc agtttgccag atgcgtggaa ggcagatgtc
     5701 ggtgcctcga cggttacgaa ggcgacggtt atcagatgtg caacatcatc cctggcgcca
     5761 ccagcgccga ctgcggcaac tgcaatggca tccccttcaa agaactggcc cagtgtgtgg
     5821 gcggcagatg tatctgcgcc agaggcttca tcgaggtgca gcccggcgtg tgcatggaat
     5881 gcgtgcagga caactgccac caggacgccg tgtgcaggcc cgacgagaga ttcaacggcg
     5941 cctacagctg tcactgcaag gccggcttca ccggggatgg ggtgtccgtg tgcaagcctg
     6001 agagcgtggg cagagaggac gccaccagca gccacaccat cgaccctacc tgcggaggcg
     6061 gctgccggac cagaaacgct gagtgcgacc ggtacaccgg aacatgcaag tgccgcagcg
     6121 gttatgacgg cgacggcgag cggggctgct actggaactg caagctctgc cacagctccg
     6181 ccatctgcga ccgcgagaac gagcggtgca tctgcccaag cgggtatagg ggcgacggcc
     6241 agaccttctg cgagcggatc cctgtgcggc aggactccat caaagtgctg atcatgggcg
     6301 agggcgaagt gatgcacatc accgacatca gccaccccct ggaactgaga tgcttcgtga
     6361 ccggcagcga ccagagctac agcggccagt ggatcaagcc ccacagcgcc caggaaccca
     6421 tcgtcaccaa gacccggcag caggacggct acgagatctc cctgctgatc aatcagccta
     6481 ccatcaccga ctccggaaga tacgagtgta gggccggcaa cgtggccgcc ttcatcgacg
     6541 tgaacgtgga agagagcgcc atccctttca ccgtgctgct gaccagcgac gacggcatcc
     6601 tgatgaccca gagcacagac agcaatgtga ccaaggccac cctgtggaat atcgccgaga
     6661 acaacaaata cggccgggtg tccatggtcg gagactgcaa ctcccagcgg atcatctaca
     6721 cctccgacta cggccacaca atcagatggg gcaacagcaa ctccggccag aacaagctgg
     6781 tcaccaaaga gatctacacc agccagttca accggttcag aaacctggcc gtggactccc
     6841 tgagcggcaa cgtgttcgcc tgggaggaag ccttcggcaa gatcatggtg ttcaacccca
     6901 acaagcccaa catccactac accctggaaa gcctgcactc cctgaccagc agcgagcagc
     6961 agaacttcgc cggactcgcc ctgcactcca ccctgggcct gatgtattgg gccatctaca
     7021 ccgatggctg gcggcccaac ggcaccattc aggtggccag catgtccggc gagaatgagg
     7081 aacagctcct gcagctgacc ggcgagcccc tggccatcag catcagcccc gccacccccg
     7141 tgaatggcaa cagcgccggc aagctgtgtt ggatgcagcg gagctccaag ggcatgttcg
     7201 gcctggtcac cgagctgcac tgcgccagac tgggccagac cggcagagag atcctgacca
     7261 acgagaagat cagagagttc agcccctccg aggaacccag cttcggcatg acccagtaca
     7321 agggcaccct gctgtggacc gacgccacca agaccgtgta ctacagcatc aaccccgatc
     7381 ggagaatcag agtcagatac gtgtgctgca gcaaccggtt ccaggccctg agcgtgctgg
     7441 gcgattgtcc ccaggccagc accaacccct gcacctacag caatggccgg tgccggtaca
     7501 tctgcctgcc taacgacggc ggcagatccc ggacctgcgt gtgtcccgac aacgagcccg
     7561 gctgccacaa agagatgggc gcgccgtcga cctccatcac ggcctataag agtgaggggg
     7621 agtcagcgga gttctccttc ccactcaacc ttggagagga aagcctgcag ggagagttga
     7681 gatggaaggc agagaaggct ccttcttccc agtcctggat caccttctcc ctaaagaacc
     7741 aaaaggtgtc tgtgcagaag tctactagca accccaagtt ccagctgtcc gaaacgctcc
     7801 cactcaccct tcagataccc caggtctccc ttcagtttgc tggttctggc aacctgaccc
     7861 tgactctgga cagagggata ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc
     7921 agcccgacag caacactttg acctgtgagg tgatgggacc cacctcaccc aagatgagac
     7981 tgatcttgaa gcaggagaat caggaggcca gggtctccag gcaggagaaa gtgattcaag
     8041 tgcaggcccc tgaagcaggg gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga
     8101 tggactccaa gatccaggtt ttatccaaag ggttgaattc cggcagcctg caccacatcc
     8161 tggacgccca gaagatgctg tggaaccaca gggacaggaa cctgcctcct ctggcccctc
     8221 tgggccccca ccaccatcac caccattgag tctagacacg tgattaatta aggatccccc
     8281 gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     8341 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg
     8401 gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     8461 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     8521 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     8581 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     8641 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     8701 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     8761 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     8821 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     8881 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     8941 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     9001 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     9061 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     9121 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     9181 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     9241 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     9301 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     9361 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     9421 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     9481 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     9541 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     9601 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     9661 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     9721 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     9781 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     9841 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     9901 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     9961 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
    10021 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
    10081 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
    10141 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
    10201 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
    10261 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
    10321 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
    10381 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
    10441 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
    10501 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa
    10561 acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
    10621 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
    10681 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
    10741 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
    10801 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
    10861 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
    10921 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
    10981 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
    11041 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    11101 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    11161 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    11221 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    11281 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
    11341 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    11401 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    11461 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    11521 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    11581 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    11641 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    11701 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    11761 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    11821 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    11881 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    11941 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    12001 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    12061 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    12121 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    12181 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    12241 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    12301 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    12361 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    12421 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    12481 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    12541 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    12601 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
    12661 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
    12721 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
    12781 cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc
    12841 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
    12901 agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag
    12961 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
    13021 atcataatat gtacatttat attggctcat gtccaatatg accgc
//
