LOCUS       sequence-234414-        7155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_181220-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1933..1950
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2002..2057
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2056..2075)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4293..4311)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4379..4483
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4484..4552,4553..5344)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4702..4721)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5515..6103
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6004..6023
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6257..6274
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6391..6412
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6427..6432,6433..6450,6451..6457)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6465..6481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6470..6492
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6489..6505
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6489..6505
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6776..7155
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccaacaagg gcacccaggt gctgagcaag
      841 cccagcacca ccaagcacct gaccacctcc acctggttca gcagcagcca ggtggaccgg
      901 cagctgaacc agttcctgac caacgagatc aagcaggaaa aggccaacgc cttcttctgc
      961 aacccccccg agggcttcaa ggtggtcaag accgacggct gcgacatcgt gatccggaaa
     1021 gagtaccagg acggcgtcag cgtggacgtg gaaatcaacc tggccggcag cgtgtccccc
     1081 agcatccccg aggatgatgt gtccgcccct gagaagatgg aagatgacgc ccccctggaa
     1141 gcccaccccg acctgcggat caagctgacc aagccctccg gccggtccct ggtgttcaac
     1201 tgcagcctgc ccggccacga cgccgacaag cagctgagcg aggacgagag caacaacctg
     1261 cggaagtggt tcctgtgcat tggcgcgccg tcgacctcca tcacggccta taagagtgag
     1321 ggggagtcag cggagttctc cttcccactc aaccttggag aggaaagcct gcagggagag
     1381 ttgagatgga aggcagagaa ggctccttct tcccagtcct ggatcacctt ctccctaaag
     1441 aaccaaaagg tgtctgtgca gaagtctact agcaacccca agttccagct gtccgaaacg
     1501 ctcccactca cccttcagat accccaggtc tcccttcagt ttgctggttc tggcaacctg
     1561 accctgactc tggacagagg gatactgtat caggaagtga acctggtggt gatgaaagtg
     1621 actcagcccg acagcaacac tttgacctgt gaggtgatgg gacccacctc acccaagatg
     1681 agactgatct tgaagcagga gaatcaggag gccagggtct ccaggcagga gaaagtgatt
     1741 caagtgcagg cccctgaagc aggggtgtgg caatgtctac tgagtgaagg tgaagaggtc
     1801 aagatggact ccaagatcca ggttttatcc aaagggttga attccggcag cctgcaccac
     1861 atcctggacg cccagaagat gctgtggaac cacagggaca ggaacctgcc tcctctggcc
     1921 cctctgggcc cccaccacca tcaccaccat tgagtctaga cacgtgatta attaaggatc
     1981 ccccgacctc gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     2041 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc
     2101 ctcggagatc tctagctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga
     2161 catctctgcc ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact
     2221 tgccaactgg gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc
     2281 tgactgtagt tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc
     2341 atcctggagc agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact
     2401 cttggctgaa gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga
     2461 ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc
     2521 ctcaagaggg cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc
     2581 atatgcttcc cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt
     2641 tacccaacgg gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc
     2701 gatatctccc accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag
     2761 ggtagtgaac cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac
     2821 tctcctgaat cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg
     2881 agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc
     2941 agaataaaat ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc
     3001 tcacaaaccc cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa
     3061 caatagaaat ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat
     3121 ttatggctat gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg
     3181 tcccaggcag ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga
     3241 aagagagtgg acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt
     3301 aaacggggct ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg
     3361 aaattgtgga gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta
     3421 aaataagggt gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact
     3481 tgcccacaaa accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca
     3541 agataggggc gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc
     3601 caagcacagg gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg
     3661 ttgccatggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata
     3721 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     3781 tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata
     3841 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta
     3901 tccgggtagc atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata
     3961 tctgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     4021 ggtagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     4081 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     4141 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4201 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctca tgataagctg
     4261 tcaaacatga gaattttctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     4321 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     4381 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4441 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4501 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4561 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4621 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4681 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     4741 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4801 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4861 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4921 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4981 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
     5041 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5101 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5161 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5221 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5281 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5341 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     5401 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5461 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5521 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5581 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5641 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5701 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5761 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5821 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5881 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5941 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6001 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6061 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6121 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6181 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6241 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     6301 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     6361 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     6421 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     6481 atttcacaca ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacca
     6541 attctcatgt ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag
     6601 gcgcagaact ggtaggtatg gaagatctat acattgaatc aatattggca attagccata
     6661 ttagtcattg gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat
     6721 ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttgacat
     6781 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     6841 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     6901 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     6961 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     7021 tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     7081 tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc
     7141 atcgctatta ccatg
//
