LOCUS       sequence-234415-        8703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_211020-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3861..3878
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3930..3985
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3984..4003)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6221..6239)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6307..6411
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6412..6480,6481..7272)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6630..6649)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7443..8031
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7932..7951
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8185..8202
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8319..8340
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8355..8360,8361..8378,8379..8385)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8393..8409
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8398..8420
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8417..8433
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8417..8433
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccctgaacg
     1201 agcacgtgca ggaaagcacc accatcctga acaaagagtg cgagaacgac gaccccctgg
     1261 gcatgatcag cggctctatc gccgactggc agatcaccag cagcagcacc taccccagca
     1321 gctgggtcaa gggctgcgag gaaaagaacg cccggctgtt ccggaccaac ggcctggctt
     1381 ggtgcgccaa gttcaagagc agcagcgagt ggctgcagat cgacctgggc gtgcaggccc
     1441 tggtcaccgg catcatgaca cagggcagag gcgacggctc cgagtgggtc accagcttca
     1501 tggtgtccta cagcgacgac gccatgcact ggaagttcgt gaccgaccag tacgccaacc
     1561 agaagatctt cgagggcaac agcgacagct acagcgtgaa gcacaactac ttcgacgagc
     1621 ccatccgggc cagattcgtg aagatccaca cctacacctg gcacaaccac cccagcctgc
     1681 gggtggaact cgtgggctgc cagagctgca agcagatcat cggcatcccc ccatacgcca
     1741 gattcgccgc cagcagctcc cggggcaagc ggagccagag aagctgcacc cccgagtacg
     1801 gccactacct gagcaacaag gcttggtgtg cccagcggca ggacgccctg cagtggctcc
     1861 agattgatgt gggccctccc accctgatca ccggcgtgat cctgaagtcc aggggcgacg
     1921 tgaagaacag ccagtacgtg acccggttca agctgagcta cagcaacgac acccggctgt
     1981 ggtacttcta caaggatgcc gcccctctgg acccccggtc caccgtgtgg tccttcgagt
     2041 ggtcccagaa gatgaaggaa agcgacgaca agatcaagag cgacaagatt tttgagggga
     2101 acaacgagca gatcaccgag cggatccact acctggccac cccctttatc gccagatacg
     2161 tgcggatcca ccccgtgatc tggcggagcc ggatcgccat gagagtgggc ctgctgggat
     2221 gcaagcagaa gggcagctgc agcgccggct tcttccggat caacaacgag agcagctgcg
     2281 tgcccaacct ggcctacaag aagaatgcct ggctgacccc cgagagcagc aaccaccgga
     2341 agcggaacct gcaggacagc gacaaggcca gcagaagcca cggcttcacc agcgagcccc
     2401 ggaacgtgat cgaccccgag aaccccagcc acttccagta caccgagaac cagaggcccc
     2461 ccatgctggc ccaccaccgg atccgggaca acaccgtgaa cagcaacgcc ttcaagttca
     2521 gcaacatccc cctgtggtcc cccgtgaacg gcatgatcaa cgagcagttc gtgggcgaga
     2581 gcagcgacgg catggccctg ctggccgtgg atggctggac cggcgaggaa atcctgctga
     2641 agaactccac cctgagcacc cggtccagcg agcggctgat gaacgacgcc cccgacaaca
     2701 agcagctgac caacgacagc ctgaacaaga aaaccatcca gaacgtggtg cacaccacct
     2761 tcgtgaccca gaagtccgcc cacagctgca ccatcctgga ataccggtgg cccttcgtgg
     2821 aactgccctc ttggtacgtg gacctgcgcg agcacatcga agtgaagggc gtggtcatct
     2881 acacagccgg ccacggccgg gacggcagat acctgctgag cagcctgttc ggctctgagg
     2941 aaaagaccaa gttcaacagc gagaacctgg aacggctgag catctacgtg gaaagcgagc
     3001 ccagacacca cgacaccaag accctgagcc ggtccagctc cagcctgtgc ggcttcgtga
     3061 ccaggatcaa cgacgccatc ttcagcccca gactgcacat cccctgccgg cagcccctga
     3121 tcggccgcta cgtgtacgtg gaagccagag gcgtgcgggg cagatggtcc caggaattcg
     3181 ccgccctgct gtgcgaagtg atggtgtatg gcgcgccgtc gacctccatc acggcctata
     3241 agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag gaaagcctgc
     3301 agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg atcaccttct
     3361 ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag ttccagctgt
     3421 ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt gctggttctg
     3481 gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac ctggtggtga
     3541 tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga cccacctcac
     3601 ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc aggcaggaga
     3661 aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg agtgaaggtg
     3721 aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat tccggcagcc
     3781 tgcaccacat cctggacgcc cagaagatgc tgtggaacca cagggacagg aacctgcctc
     3841 ctctggcccc tctgggcccc caccaccatc accaccattg agtctagaca cgtgattaat
     3901 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     3961 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     4021 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     4081 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     4141 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     4201 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     4261 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     4321 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     4381 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     4441 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     4501 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     4561 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     4621 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     4681 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     4741 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     4801 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     4861 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     4921 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     4981 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     5041 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     5101 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     5161 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     5221 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     5281 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     5341 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     5401 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     5461 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     5521 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     5581 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     5641 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     5701 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     5761 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     5821 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     5881 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     5941 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     6001 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     6061 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     6121 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
     6181 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
     6241 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     6301 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6361 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6421 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6481 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6541 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6601 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     6661 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6721 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6781 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6841 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6901 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     6961 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7021 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7081 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7141 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7201 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7261 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7321 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7381 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7441 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7501 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7561 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7621 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7681 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7741 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7801 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7861 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7921 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7981 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8041 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8101 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8161 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     8221 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     8281 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     8341 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     8401 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
     8461 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
     8521 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat
     8581 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
     8641 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgccat
     8701 gtt
//
