LOCUS       sequence-234420-        8625 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LYS2 ICE plasmid used to facilitate yeast recombination cloning and 
            stable genomic Integration after CEN Excision..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8625)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 8625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: pICE LYS2
FEATURES             Location/Qualifiers
     source          1..8625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..37
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     21..37
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(71..87)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(72..88)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(112..131)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(113..131)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(141..158)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(141..157)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(150..172)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      302..757
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(389..408)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     599..620
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        811..1050
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1051..5229
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(5627..5646)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5746..5768
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5806..5824)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5892..5996
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5997..6065,6066..6857)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6215..6234)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7028..7616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7517..7536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7770..7787
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(7940..7945,7946..7963,7964..7970)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7978..7994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7983..8005
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8002..8018
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8002..8018
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8037..8057
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8039..8057
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    8112..8615
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 agcgctaaga gggcggccgc tctagaacta gtggatcccc cgggctgcag gaattcgata
       61 tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccaattc gccctatagt
      121 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      181 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      241 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      301 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      361 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      421 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      481 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      541 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      601 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      661 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      721 aacgcgaatt ttaacaaaat attaacgttt acaatttcct gatgcggtat tttctcctta
      781 cgcatctgtg cggtatttca caccgcatat aattccactt gcaattacat aaaaaattcc
      841 ggcggttttt cgcgtgtgac tcaatgtcga aatacctgcc taatgaacat gaacatcgcc
      901 caaatgtatt tgaagacccg ctgggagaag ttcaagatat ataagtaaca agcagccaat
      961 agtataaaaa aaaatctgag tttattacct ttcctggaat ttcagtgaaa aactgctaat
     1021 tatagagaga tatcacagag ttactcacta atgactaacg aaaaggtctg gatagagaag
     1081 ttggataatc caactctttc agtgttacca catgactttt tacgcccaca acaagaacct
     1141 tatacgaaac aagctacata ttcgttacag ctacctcagc tcgatgtgcc tcatgatagt
     1201 ttttctaaca aatacgctgt cgctttgagt gtatgggctg cattgatata tagagtaacc
     1261 ggtgacgatg atattgttct ttatattgcg aataacaaaa tcttaagatt caatattcaa
     1321 ccaacgtggt catttaatga gctgtattct acaattaaca atgagttgaa caagctcaat
     1381 tctattgagg ccaatttttc ctttgacgag ctagctgaaa aaattcaaag ttgccaagat
     1441 ctggaaagga cccctcagtt gttccgtttg gcctttttgg aaaaccaaga tttcaaatta
     1501 gacgagttca agcatcattt agtggacttt gctttgaatt tggataccag taataatgcg
     1561 catgttttga acttaattta taacagctta ctgtattcga atgaaagagt aaccattgtt
     1621 gcggaccaat ttactcaata tttgactgct gcgctaagcg atccatccaa ttgcataact
     1681 aaaatctctc tgatcaccgc atcatccaag gatagtttac ctgatccaac taagaacttg
     1741 ggctggtgcg atttcgtggg gtgtattcac gacattttcc aggacaatgc tgaagccttc
     1801 ccagagagaa cctgtgttgt ggagactcca acactaaatt ccgacaagtc ccgttctttc
     1861 acttatcgcg acatcaaccg cacttctaac atagttgccc attatttgat taaaacaggt
     1921 atcaaaagag gtgatgtagt gatgatctat tcttctaggg gtgtggattt gatggtatgt
     1981 gtgatgggtg tcttgaaagc cggcgcaacc ttttcagtta tcgaccctgc atatccccca
     2041 gccagacaaa ccatttactt aggtgttgct aaaccacgtg ggttgattgt tattagagct
     2101 gctggacaat tggatcaact agtagaagat tacatcaatg atgaattgga gattgtttca
     2161 agaatcaatt ccatcgctat tcaagaaaat ggtaccattg aaggtggcaa attggacaat
     2221 ggcgaggatg ttttggctcc atatgatcac tacaaagaca ccagaacagg tgttgtagtt
     2281 ggaccagatt ccaacccaac cctatctttc acatctggtt ccgaaggtat tcctaagggt
     2341 gttcttggta gacatttttc cttggcttat tatttcaatt ggatgtccaa aaggttcaac
     2401 ttaacagaaa atgataaatt cacaatgctg agcggtattg cacatgatcc aattcaaaga
     2461 gatatgttta caccattatt tttaggtgcc caattgtatg tccctactca agatgatatt
     2521 ggtacaccgg gccgtttagc ggaatggatg agtaagtatg gttgcacagt tacccattta
     2581 acacctgcca tgggtcaatt acttactgcc caagctacta caccattccc taagttacat
     2641 catgcgttct ttgtgggtga cattttaaca aaacgtgatt gtctgaggtt acaaaccttg
     2701 gcagaaaatt gccgtattgt taatatgtac ggtaccactg aaacacagcg tgcagtttct
     2761 tatttcgaag ttaaatcaaa aaatgacgat ccaaactttt tgaaaaaatt gaaagatgtc
     2821 atgcctgctg gtaaaggtat gttgaacgtt cagctactag ttgttaacag gaacgatcgt
     2881 actcaaatat gtggtattgg cgaaataggt gagatttatg ttcgtgcagg tggtttggcc
     2941 gaaggttata gaggattacc agaattgaat aaagaaaaat ttgtgaacaa ctggtttgtt
     3001 gaaaaagatc actggaatta tttggataag gataatggtg aaccttggag acaattctgg
     3061 ttaggtccaa gagatagatt gtacagaacg ggtgatttag gtcgttatct accaaacggt
     3121 gactgtgaat gttgcggtag ggctgatgat caagttaaaa ttcgtgggtt cagaatcgaa
     3181 ttaggagaaa tagatacgca catttcccaa catccattgg taagagaaaa cattacttta
     3241 gttcgcaaaa atgccgacaa tgagccaaca ttgatcacat ttatggtccc aagatttgac
     3301 aagccagatg acttgtctaa gttccaaagt gatgttccaa aggaggttga aactgaccct
     3361 atagttaagg gcttaatcgg ttaccatctt ttatccaagg acatcaggac tttcttaaag
     3421 aaaagattgg ctagctatgc tatgccttcc ttgattgtgg ttatggataa actaccattg
     3481 aatccaaatg gtaaagttga taagcctaaa cttcaattcc caactcccaa gcaattaaat
     3541 ttggtagctg aaaatacagt ttctgaaact gacgactctc agtttaccaa tgttgagcgc
     3601 gaggttagag acttatggtt aagtatatta cctaccaagc cagcatctgt atcaccagat
     3661 gattcgtttt tcgatttagg tggtcattct atcttggcta ccaaaatgat ttttacctta
     3721 aagaaaaagc tgcaagttga tttaccattg ggcacaattt tcaagtatcc aacgataaag
     3781 gcctttgccg cggaaattga cagaattaaa tcatcgggtg gatcatctca aggtgaggtc
     3841 gtcgaaaatg tcactgcaaa ttatgcggaa gacgccaaga aattggttga gacgctacca
     3901 agttcgtacc cctctcgaga atattttgtt gaacctaata gtgccgaagg aaaaacaaca
     3961 attaatgtgt ttgttaccgg tgtcacagga tttctgggct cctacatcct tgcagatttg
     4021 ttaggacgtt ctccaaagaa ctacagtttc aaagtgtttg cccacgtcag ggccaaggat
     4081 gaagaagctg catttgcaag attacaaaag gcaggtatca cctatggtac ttggaacgaa
     4141 aaatttgcct caaatattaa agttgtatta ggcgatttat ctaaaagcca atttggtctt
     4201 tcagatgaga agtggatgga tttggcaaac acagttgata taattatcca taatggtgcg
     4261 ttagttcact gggtttatcc atatgccaaa ttgagggatc caaatgttat ttcaactatc
     4321 aatgttatga gcttagccgc cgtcggcaag ccaaagttct ttgactttgt ttcctccact
     4381 tctactcttg acactgaata ctactttaat ttgtcagata aacttgttag cgaagggaag
     4441 ccaggcattt tagaatcaga cgatttaatg aactctgcaa gcgggctcac tggtggatat
     4501 ggtcagtcca aatgggctgc tgagtacatc attagacgtg caggtgaaag gggcctacgt
     4561 gggtgtattg tcagaccagg ttacgtaaca ggtgcctctg ccaatggttc ttcaaacaca
     4621 gatgatttct tattgagatt tttgaaaggt tcagtccaat taggtaagat tccagatatc
     4681 gaaaattccg tgaatatggt tccagtagat catgttgctc gtgttgttgt tgctacgtct
     4741 ttgaatcctc ccaaagaaaa tgaattggcc gttgctcaag taacgggtca cccaagaata
     4801 ttattcaaag actacttgta tactttacac gattatggtt acgatgtcga aatcgaaagc
     4861 tattctaaat ggaagaaatc attggaggcg tctgttattg acaggaatga agaaaatgcg
     4921 ttgtatcctt tgctacacat ggtcttagac aacttacctg aaagtaccaa agctccggaa
     4981 ctagacgata ggaacgccgt ggcatcttta aagaaagaca ccgcatggac aggtgttgat
     5041 tggtctaatg gaataggtgt tactccagaa gaggttggta tatatattgc atttttaaac
     5101 aaggttggat ttttacctcc accaactcat aatgacaaac ttccactgcc aagtatagaa
     5161 ctaactcaag cgcaaataag tctagttgct tcaggtgctg gtgctcgtgg aagctccgca
     5221 gcagcttaag gttgagcatt acgtatgata tgtccatgta caataattaa atatgaatta
     5281 ggagaaagac ttagcttctt ttcgggtgat gtcacttaaa aactccgaga ataatatata
     5341 ataagagaat aaaatattag ttattgaata agaactgtaa atcagctggc gttagtctgc
     5401 taatggcagc ttcatcttgg tttattgtag catgaatcat atttgccttt ttttcctgta
     5461 attcaatgat tcttgcttct atactatcct caatgcaaaa ccttgtgatc ttcacaggtc
     5521 gatactgacc aattctatga actctatcac cactttgcca ttcaacacta gggttccacc
     5581 atgggtctaa aatgaatact tgcgaagcta tatggtgcac tctcagtaca atctgctctg
     5641 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5701 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5761 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5821 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5881 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5941 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6001 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6061 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6121 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6181 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6241 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6301 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6361 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6421 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6481 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6541 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6601 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6661 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6721 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6781 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6841 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6901 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6961 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7021 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7081 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7141 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     7201 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7261 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7321 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7381 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7441 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7501 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7561 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7621 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7681 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7741 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7801 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     7861 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttacctca
     7921 ctcattaggc accccaggct ttacacttta tgcttccggc tcctatgttg tgtggaattg
     7981 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     8041 ttaaccctca ctaaagggaa caaaagctgg agctccaccg cggtggcggc cgcgccacct
     8101 gggtcctttt catcacgtgc tataaaaata attataattt aaatttttta atataaatat
     8161 ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt tgttttccga
     8221 agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt gctcttcctg
     8281 ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca cacacgaaaa
     8341 tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta atctgctttt
     8401 cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta aacctttgtt
     8461 tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc taaaatccaa
     8521 atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc atcattaaaa
     8581 gatacgaggc gcgtgtaagt tacaggcaag cgatccgtcc taaga
//
