LOCUS       sequence-234462-        4704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cyanobacteria cloning vector. Chromosomal integration into 
            Synechococcus elongatus neutral site 3 (NS3)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria.
  JOURNAL   Nucleic Acids Res. 2014 Jul 29. pii: gku673.
  PUBMED    25074377
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jul 29. pii: gku673."
COMMENT     Sequence Label: pAM4941 (pCV0053)
FEATURES             Location/Qualifiers
     source          1..4704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      845..1433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1334..1353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1587..1604
                     /label=L4440
                     /note="L4440 vector, forward primer"
     oriT            1674..1783
                     /note="incP origin of transfer"
     CDS             complement(2937..3470)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3659..3664,3665..3681,3682..3687))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4269..4574
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4453..4472
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      4642..4669
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tctagacgtg agccgtagtc ccgtcgccag acgcgggtgc ccacgggcgt cgtcaggatg
       61 tccgtaattg actgccggag gtggtcaatg cccttcagct ccttgccact gtcacggctc
      121 atgcctcggg tcattagtcg cccgctccgg tatcttcact ggcttcgatg attgccgccc
      181 cgcagctgca gaggtcaccg atccgagcag tcggcctctg gttggtaaag accgtgcgac
      241 tgccggtgat gatcgtgttc aagccatgca gggggcaggc gtgaaggtca tcctttcggg
      301 ccacggggcg gctgttgacg aaggtgtcgt cgctcccggt gatgatgatc ccgccgtgat
      361 cggtcacgtc gtttagtcga gcgatgcctg gcgtcgtagt cacgggttta ggtcaatacg
      421 acttgcggtc actgtaacgt tgccctcggc ggtcacgtta acgtcgcctt gggcttcgac
      481 ttgcgcctcc tgcacaagga tcacaatccg tccttgggct gcggtgaggt cgatcttgta
      541 ctcatgcgct tcgcggtcgt actggatgat tgagtcatcc tcgaactgcg tcttttggat
      601 cgtttctttg tcctcgatct gggggtagtc agtcgagaac gcgccgggca tcgcgaagcc
      661 ctgactgatc tcgccggagg gggccatcac gacgacggcc tcaccgacct cgggcgccca
      721 ccagaaccga tccttgcccg ctcgctgcgt gagccacgga atccagtcag tgaggagcag
      781 cgcctcgccg ctctcctcgt cttcctcgag cgtcagaccc cgtagaaaag atcaaaggat
      841 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      901 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      961 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1021 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1081 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1141 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1201 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1261 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1321 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1381 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1441 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1501 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     1561 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagactggt
     1621 tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca
     1681 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa
     1741 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat
     1801 acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga
     1861 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaagatcc
     1921 ctgacgggct tgtctgctcc cgccatccgc ttacagacaa gctccgcgcg tcgtgatgtc
     1981 gaaacccaag ccaccctcag aggtgaaggc cgcttcgagc acatcgggaa gcgtgtcgac
     2041 gacggtgctc ggtgcctcgg gtaagagcca gatagatgcg gtgacgttca ccgtcgcgat
     2101 cgtggcggga accacggtca ctgcgtccgt tacaactcgc acgtcgtcgg ccagcacgac
     2161 tgactcgacg gcttcgatta gcccgggaga ggcaaggcca tcaggctcgg tagacagaat
     2221 gctgattaac acctcgccgg gagcagggct gctcacagcc gcgtccttca cccgtgggtc
     2281 agccgtcagc gcttgatagc ggtaccaggc cgcaccgcct gcggtcgagc tgcccttgat
     2341 ccgctcgatg gtgcgatcgc gaagctcgcc atccggctca ttcggctgcc gcaccacgcc
     2401 gtagaacgcg gcaaggttgt cgaggtcggg gccgtttgag tagcgaagca gtgtcgcaag
     2461 aagtgcgtcg ttgatccgct gacgcaggat cagctcgcgg gctgcgcaga cctccagcag
     2521 cttgatgacc gggtcggatt cgaggatggc ggtgtagctg gcgtcacgcg atcgcaggtc
     2581 gtcgatcaag tcctgcagga tcagttcaaa gtccagcgct tcgatgatgg tgggcgcggg
     2641 aatcgtagca aagtcaagaa cggtcatgag acgactaagc cctccagcgt gatacgcctg
     2701 ccctcgggga tgtagtagcc gatcaggttc agctcgactt gaccagctgc gctggctgag
     2761 acgatgcgaa ccttctccag cttcagccgt ggctcccagc gatcgagcgc ttcagctgtg
     2821 gccgccacca ggtcaacgat gagggactgg ttgatcggtc gctagcatcg cgcgcgcgcg
     2881 cgcggatgct agcggcgcgc caaaaaaaag cccgctcatt aggcgggctg gccggcttag
     2941 gtggcggtac ttgggtcaat atcaaagtgc atcacttctt cccgtatgcc caactttgta
     3001 tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca
     3061 agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg
     3121 tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg
     3181 ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg
     3241 atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag
     3301 taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg
     3361 acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt
     3421 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct
     3481 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
     3541 agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc
     3601 gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag
     3661 tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt
     3721 gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa
     3781 cgagcgcaag gtttcggtct ccacgaccgg tatcccgccg ccgccgccgg ataccggtta
     3841 atagtacctg tgaagtgaaa aatggcgcac attgtgcgac atttttttct cgagcaggta
     3901 gtcggccaat aacccaggga tttaaatacg cgtggatccg gcttactaaa agccagataa
     3961 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     4021 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     4081 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     4141 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     4201 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     4261 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     4321 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     4381 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     4441 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     4501 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     4561 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgacatttaa
     4621 atgaaggagc tccccggcag gagaaggcca tcctgacgga tggccttttt gaagctttct
     4681 agaatcgggg gcccccgggg ggat
//
