LOCUS       sequence-234474-        7038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_202610-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2196..2213
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2265..2320
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2319..2338)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4556..4574)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4642..4746
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4747..4815,4816..5607)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4965..4984)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5778..6366
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6267..6286
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6520..6537
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6654..6675
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6690..6695,6696..6713,6714..6720)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6728..6744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6733..6755
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6752..6768
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6752..6768
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccgtgaagt
     1201 ccgaggaata cgcccagatg agcatcctga acaccgacca gcccgactgc cacttcctgt
     1261 gcgagctgtg caccagcacc gtgaacgcca ccaagtacat cctggacaac gagcccttca
     1321 tccccgagat cctgaagaac ttcatgccca tctgctacat gctgcccaag ctgcagtacc
     1381 ggaagatctg ctaccacttc gtgaacggcg gagccgtgga cctgatccac gagctggtca
     1441 acaccatcaa cgagaacaat ttctgcttcc acctgggcct gtgcagctcc accatcccct
     1501 gccccagctt ccagaaaacc ctgacctaca acgacctgag cacaggcgcg ccgtcgacct
     1561 ccatcacggc ctataagagt gagggggagt cagcggagtt ctccttccca ctcaaccttg
     1621 gagaggaaag cctgcaggga gagttgagat ggaaggcaga gaaggctcct tcttcccagt
     1681 cctggatcac cttctcccta aagaaccaaa aggtgtctgt gcagaagtct actagcaacc
     1741 ccaagttcca gctgtccgaa acgctcccac tcacccttca gataccccag gtctcccttc
     1801 agtttgctgg ttctggcaac ctgaccctga ctctggacag agggatactg tatcaggaag
     1861 tgaacctggt ggtgatgaaa gtgactcagc ccgacagcaa cactttgacc tgtgaggtga
     1921 tgggacccac ctcacccaag atgagactga tcttgaagca ggagaatcag gaggccaggg
     1981 tctccaggca ggagaaagtg attcaagtgc aggcccctga agcaggggtg tggcaatgtc
     2041 tactgagtga aggtgaagag gtcaagatgg actccaagat ccaggtttta tccaaagggt
     2101 tgaattccgg cagcctgcac cacatcctgg acgcccagaa gatgctgtgg aaccacaggg
     2161 acaggaacct gcctcctctg gcccctctgg gcccccacca ccatcaccac cattgagtct
     2221 agacacgtga ttaattaagg atcccccgac ctcgacctct ggctaataaa ggaaatttat
     2281 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     2341 ggcaaatcat ttggtcgaga tccctcggag atctctagct agaggatcga tccccgcccc
     2401 ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat
     2461 cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg
     2521 ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca
     2581 tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca
     2641 caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg
     2701 tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct
     2761 gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc
     2821 cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact
     2881 aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta
     2941 gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt
     3001 acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga
     3061 agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg
     3121 tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa
     3181 acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt
     3241 gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa
     3301 tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga
     3361 ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat
     3421 gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt
     3481 acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt
     3541 tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag
     3601 acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg
     3661 ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg
     3721 ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc
     3781 tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat
     3841 tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc
     3901 gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat
     3961 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     4021 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     4081 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4141 agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt
     4201 agtatatgct atcctaattt atatctgggt agcatatact acccaaatat ctggatagca
     4261 tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca
     4321 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     4381 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
     4441 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
     4501 tatgctatcc tcatgataag ctgtcaaaca tgagaatttt cttgaagacg aaagggcctc
     4561 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4621 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4681 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4741 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4801 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4861 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4921 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4981 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5041 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5101 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5161 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5221 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5281 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5341 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5401 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5461 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5521 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5581 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5641 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5701 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5761 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5821 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5881 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5941 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6001 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6061 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6121 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6181 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6241 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6301 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6361 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6421 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6481 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6541 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6601 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     6661 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     6721 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     6781 aagctctagc tagaggtcga ccaattctca tgtttgacag cttatcatcg cagatccggg
     6841 caacgttgtt gccattgctg caggcgcaga actggtaggt atggaagatc tatacattga
     6901 atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc aatattggct
     6961 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
     7021 caatatgacc gccatgtt
//
