LOCUS       sequence-234475-        8133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_002600-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2911..2928
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2980..3035
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3034..3053)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5271..5289)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5357..5461
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5462..5530,5531..6322)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5680..5699)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6493..7081
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6982..7001
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7235..7252
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7369..7390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7405..7410,7411..7428,7429..7435)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7443..7459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7448..7470
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7467..7483
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7467..7483
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7754..8133
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccctgctga ccagacactt cccctggtcc
      841 accatcaaga accccgactt cacctacgag acaaagtact tctggacccg ggtggaccac
      901 ttcagcttcg tgaacgacga gaagttcctg atccgctacc tgatcaacaa cgagagcttc
      961 acccctggcg gccctatcct gttctacacc ggcaacgagg gccccatcga gacattcgcc
     1021 gagaacagcg gcttcatctg gaagctgagc cgcgagctga acgccagcgt ggtgttcgcc
     1081 gagcaccggt actacggcac cagcctgccc ttcggcaaca acagcttcaa ggaccggcgg
     1141 cacttcggct acctgaccgc cgagcagacc ctggccgact acgtgctgct gatcaaccag
     1201 ctgaaggcca actacagctg cttcgccagc agccccgtga tcgcctttgg cggcagctac
     1261 ggcggcatgc tgagcgcctg gatccggcag aagtacccca accagatcgc tggcgccatt
     1321 gccagcagcg cccctgtgtg gctgttcccc ggcctgagcg actgcaacgg cttcagcatg
     1381 accatcacca acagctttct gaagtacggc ggcgagaact gcgtgaagaa catccagctg
     1441 agctggtcca acatcgtgga catcggccag agcgtggacg gcaaagaact gctgacccac
     1501 atgttcaaca tctgcacccc cctgaccgac gtgcagaaca tcatcgacta cctgtccgac
     1561 ttcctgggca tcatcagcat ggtcaactac ccctaccccg ccagcctgat cctggccctg
     1621 cctgagtggc ccgtgaagta cctgtgcacc aacctgagcg agtacgaccc ccagcagccc
     1681 gtggtcacca gaatcagcct gctggccaag gccgtgctga gcctgaccaa ctacacaggc
     1741 aaccagacct gcctggacat cagcatgaac ctgcccaacg tggacgtgaa cggctgggac
     1801 ctgcaggcct gtatggaaat ggtcacccct acctgcgcca gcggccctgt gaacatcatg
     1861 ccccccagca actgggacct gaaaacctac agcatctact gccagaacag attcggcgtg
     1921 tcccccagag tggaatggcc caaggtggaa ttctggtcca agagcgtgta caccgtgacc
     1981 aacatcatct tcagcaacgg cgagatcgac ccttggagcg ccttcagcat cacaaacaac
     2041 agctacgtgc ccttcgccac cgtgatcaat atgagcgacg ccgcccacca cctggacctg
     2101 agaaccccca acagcgccga cccccagtcc gtggtggaag ccagagagat cgagaagcag
     2161 aaaatcatcc agtggatcaa agagtggaac ctgaagtaca agctgctcaa gatcttcaac
     2221 ggcatcgtgt ccttcatctc cctgctggac tacctgggag gcgcgccgtc gacctccatc
     2281 acggcctata agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag
     2341 gaaagcctgc agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg
     2401 atcaccttct ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag
     2461 ttccagctgt ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt
     2521 gctggttctg gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac
     2581 ctggtggtga tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga
     2641 cccacctcac ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc
     2701 aggcaggaga aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg
     2761 agtgaaggtg aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat
     2821 tccggcagcc tgcaccacat cctggacgcc cagaagatgc tgtggaacca cagggacagg
     2881 aacctgcctc ctctggcccc tctgggcccc caccaccatc accaccattg agtctagaca
     2941 cgtgattaat taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca
     3001 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     3061 atcatttggt cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg
     3121 aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt
     3181 cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc
     3241 aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc
     3301 caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca
     3361 ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct
     3421 cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta
     3481 gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt
     3541 taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc
     3601 taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa
     3661 agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg
     3721 gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc
     3781 aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag
     3841 ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag
     3901 gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct
     3961 atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa
     4021 cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga
     4081 tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac
     4141 tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact
     4201 ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct
     4261 ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag
     4321 tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc
     4381 ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac
     4441 cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat
     4501 aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac
     4561 acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga
     4621 gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat
     4681 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     4741 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     4801 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     4861 atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat
     4921 atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc
     4981 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc
     5041 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     5101 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     5161 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     5221 tatcctcatg ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat
     5281 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     5341 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5401 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5461 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5521 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5581 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5641 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5701 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5761 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5821 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5881 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5941 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6001 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6061 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6121 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6181 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6241 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6301 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6361 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6421 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6481 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6541 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6601 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6661 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6721 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6781 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6841 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6901 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6961 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7021 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7081 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7141 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7201 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7261 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     7321 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     7381 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     7441 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     7501 ctagctagag gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg
     7561 ttgttgccat tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa
     7621 tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg
     7681 ccattgcata cgttgtatct atatcataat atgtacattt atattggctc atgtccaata
     7741 tgaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     7801 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     7861 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     7921 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     7981 ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt
     8041 aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag
     8101 tacatctacg tattagtcat cgctattacc atg
//
