LOCUS       sequence-234476-        7065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_147730-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1843..1860
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1912..1967
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1966..1985)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4203..4221)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4289..4393
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4394..4462,4463..5254)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4612..4631)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5425..6013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5914..5933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6167..6184
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6301..6322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6337..6342,6343..6360,6361..6367)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6375..6391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6380..6402
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6399..6415
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6399..6415
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6686..7065
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gcctacagaa agggcaacag cgactgcctg
      841 ctggactacg acgagggcat ctgtcgggcc ctgctgaagc ggttctacta cgacagcgtg
      901 aaccagacct gcgagatctt ctactacggc ggctgcctgg gcaacggcaa caacttcctg
      961 agcaaagagg aatgcgagcg gaagtgtggc ggccagatct acatgaccga gaagtccttc
     1021 gaaaccacca agcagatgga aaccaccagc accagcatcg acagaagcga caacaccgag
     1081 acaaccatca ccacccagaa acccctgagc gtgggcgcca agatcgtgct gggcatcctg
     1141 gacatcaaga acaaggtgtc caacctgttc aagaagatca agggcgagaa aggcgcgccg
     1201 tcgacctcca tcacggccta taagagtgag ggggagtcag cggagttctc cttcccactc
     1261 aaccttggag aggaaagcct gcagggagag ttgagatgga aggcagagaa ggctccttct
     1321 tcccagtcct ggatcacctt ctccctaaag aaccaaaagg tgtctgtgca gaagtctact
     1381 agcaacccca agttccagct gtccgaaacg ctcccactca cccttcagat accccaggtc
     1441 tcccttcagt ttgctggttc tggcaacctg accctgactc tggacagagg gatactgtat
     1501 caggaagtga acctggtggt gatgaaagtg actcagcccg acagcaacac tttgacctgt
     1561 gaggtgatgg gacccacctc acccaagatg agactgatct tgaagcagga gaatcaggag
     1621 gccagggtct ccaggcagga gaaagtgatt caagtgcagg cccctgaagc aggggtgtgg
     1681 caatgtctac tgagtgaagg tgaagaggtc aagatggact ccaagatcca ggttttatcc
     1741 aaagggttga attccggcag cctgcaccac atcctggacg cccagaagat gctgtggaac
     1801 cacagggaca ggaacctgcc tcctctggcc cctctgggcc cccaccacca tcaccaccat
     1861 tgagtctaga cacgtgatta attaaggatc ccccgacctc gacctctggc taataaagga
     1921 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     1981 atgggagggc aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc
     2041 ccgccccgga cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc
     2101 atgtaatccc ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca
     2161 cgggggggga ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat
     2221 gtgcacattt gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac
     2281 tgcaacacaa cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg
     2341 ggacatgtac ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga
     2401 ggggcctgtg tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata
     2461 aggccccctt gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat
     2521 ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt
     2581 agggttagta aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt
     2641 tttatttaca tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag
     2701 tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc
     2761 tccttcgttt agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg
     2821 ctcgaaaaca aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg
     2881 tggcattgtg ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt
     2941 gtaggaatga aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc
     3001 gtaaagactg gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt
     3061 gcaatatgat actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca
     3121 tgttgttaca ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc
     3181 cactggttgt ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata
     3241 aacaaagaca agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc
     3301 ccacacgccg ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt
     3361 aaccccgcta accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg
     3421 gaatacctgc ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag
     3481 gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca
     3541 cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata
     3601 tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
     3661 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
     3721 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     3781 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga
     3841 ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg
     3901 gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     3961 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4021 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     4081 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     4141 ggtagcatat gctatcctca tgataagctg tcaaacatga gaattttctt gaagacgaaa
     4201 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4261 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4321 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4381 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4441 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4501 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4561 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4621 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     4681 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4741 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4801 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4861 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4921 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4981 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5041 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5101 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5161 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5221 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5281 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5341 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5401 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5461 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5521 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5581 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5641 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5701 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5761 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5821 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5881 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5941 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6001 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6061 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6121 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6181 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     6241 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     6301 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     6361 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     6421 ttacgccaag ctctagctag aggtcgacca attctcatgt ttgacagctt atcatcgcag
     6481 atccgggcaa cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat
     6541 acattgaatc aatattggca attagccata ttagtcattg gttatatagc ataaatcaat
     6601 attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc
     6661 tcatgtccaa tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca
     6721 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     6781 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     6841 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     6901 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac
     6961 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt
     7021 cctacttggc agtacatcta cgtattagtc atcgctatta ccatg
//
